Crystallography Reports

, 54:920 | Cite as

X-ray fluorescence studies for the elemental composition and molecular organization of protein films on the surface of the liquid subphase

  • S. I. Zheludeva
  • N. N. Novikova
  • M. V. Kovalchuk
  • N. D. Stepina
  • O. V. Konovalov
  • É. A. Yurieva


This paper reports on the results of the investigation of protein films that are based on alkaline phosphatase and glucose oxidase enzymes and formed on the surface of the liquid subphase. The experimental studies have been performed using total external reflection X-ray fluorescence spectrometry at the European Synchrotron Radiation Facility (Grenoble, France). The self-organization processes that occur in protein systems on the surface of the liquid subphase under the conditions where the protein molecules retain their mobility have been investigated using X-ray fluorescence measurements for the first time.


Glucose Oxidase DPPC Crystallography Report Fluorescence Yield Lipid Film 
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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • S. I. Zheludeva
    • 1
  • N. N. Novikova
    • 2
  • M. V. Kovalchuk
    • 2
  • N. D. Stepina
    • 1
  • O. V. Konovalov
    • 3
  • É. A. Yurieva
    • 4
  1. 1.Shubnikov Institute of CrystallographyRussian Academy of SciencesMoscowRussia
  2. 2.Kurchatov Centre for Synchrotron Radiation and NanotechnologyRussian Research Centre “Kurchatov Institute,”MoscowRussia
  3. 3.European Synchrotron Radiation FacilityGrenobleFrance
  4. 4.Moscow Research Institute of Pediatrics and Child SurgeryMinistry of Health of the Russian FederationMoscowRussia

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