The Composition of Carotenoids in Tissues of the Ascidian Botryllus schlosseri (Pallas, 1766) from the Black Sea
- 9 Downloads
The composition of carotenoids was investigated in tissues of the colonial sea squirt Botryllus schlosseri (Pallas, 1766), which inhabits the Crimean coast near the city of Sevastopol. The total carotenoid amount of B. schlosseri was found to be 8.7 ± 4.6 mg/100 g of wet weight. Eleven carotenoids, that is, ß-carotene, 7,8-didehydroastaxanthin, 7,8,7',8',-tetradehydroastaxanthin, pectenolone, 4-ketoalloxanthin, diatoxanthin, diadinoxanthin, fucoxanthin, astaxanthin, alloxanthin, and halocynthiaxanthin, were identified in B. schlosseri with the use of the chromatographic and mass-spectrophotometric methods (UV/Vis, ESITOF/MS, and partially 1H-NMR). Among these, halocynthiaxanthin (20.8% of the total content of carotenoids), alloxanthin (15.2%), and astaxanthin (12.1%) were found to be the major carotenoids. A comparative analysis of the composition of carotenoids in tissues of B. schlosseri was carried out for different regions of the World Ocean.
Keywordscarotenoids, tunicates Botryllus schlosseri Black Sea astaxanthin alloxanthin halocynthiaxanthin
Unable to display preview. Download preview PDF.
- 1.Karnaukhov, V.N., Biologicheskie funktsii karotinoidov (Biological Functions of Carotenoids), Moscow: Nauka, 1988.Google Scholar
- 2.Motorya, E.S., Technology of biologically active food additive based on carotenoids of the ascidian Halocynthia aurantium, Extended Abstract of Cand. Sci. (Tech.) Dissertation, Vladivostok: Far Eastern Scientific Center, 2009.Google Scholar
- 3.Motorya, E.S., Pivnenko, TN, Zadorozhny, P.A., et al., Identification of carotenoids of the tunic of the purple sea squirt Halocynthia aurantium, Izv. Tikhookean. Nauchno-Issled. Inst. Rybn. Khoz. Okeanogr.,, 2011, vol. 167, pp. 252–261.Google Scholar
- 4.Senicheva, M.I., Species diversity, seasonal and interannual variability of microalgae in the plankton off the coast of the Crimea, in Mikrovodorosli Chernogo morya: problemy bioraznoobraziya, sokhraneniya i biotekhnologisheskogo ispol’zovaniya (Microalgae of the Black Sea: Problems of Biodiversity, Conservation and Biotechnological Use), Sevastopol: EKOSI-Gidrofizika, 2008, pp. 5–18.Google Scholar
- 6.Englert, G., NMR Spectroscopy, in Carotenoids, Britton, G., Liaaen-Jensen, S., Pfander, H.P., Eds., Basel: Birkhäuser, 1995, vol. 1B, pp. 147–260.Google Scholar
- 7.Eugster, C.H., Chemical derivatization: microscale tests for the presence of common functional groups in carotenoids, in Carotenoids, Isolation and Analysis, Basel: Birkhäuser, 1995, vol. 1A, pp. 71–80.Google Scholar
- 8.Jeffrey, S.W., Mantoura, R.F.C., and Bjørnland, T., Data for the identification of 47 key phytoplankton pigments, in Phytoplankton Pigments in Oceanography: Guidelines to Modern Method, Monographs on Oceanographic Methodology Series, vol. 10, Paris: UNESCO Publ., 1997, pp. 449–559.Google Scholar
- 9.Maoka, T., Structural studies of carotenoids in plants, animals, and food products, in Carotenoids: Nutrition, Analysis and Technology, Chichester: Wiley, 2016, pp. 103–129.Google Scholar
- 10.Maoka, T. and Akimoto, N., Natural product chemistry in carotenoid, some experimental techniques for structural elucidation and analysis of natural carotenoids, Carotenoid Sci., 2008, vol. 13, pp. 10–17.Google Scholar
- 11.Matsuno, T., Ookubo, M., and Komori, T., Carotenoids of tunicates. III: The structural elucidation of two new marine carotenoids, amarouciaxanthin A and B, J. Nat. Prod., 1985, vol. 48, no. 4, pp. 606–613.Google Scholar
- 14.Nishino, H., Satomi, Y., Tokuda, H., et al., Anti-tumor activity of peridinin and its structurally related butenolide compounds, J. Kyoto Prefect. Univ. Med., 1991, vol. 100, pp. 831–835.Google Scholar