Russian Journal of Marine Biology

, Volume 37, Issue 6, pp 421–429 | Cite as

The morphology, development, and state of the photosynthetic apparatus of the diatom Attheya ussurensis Stonik, Orlova et Crawford, 2006 (Bacillariophyta) in long-term culture

  • T. Yu. Orlova
  • N. A. Aizdaicher
  • I. V. Stonik
  • O. G. Schevchenko
  • S. I. Pogosyan


The development of the marine benthic diatom Attheya ussurensis (Bacillariophyta) isolated from Ussuriisky Bay (Sea of Japan) was examined in laboratory culture. It was found that the development of A. ussurensis was characterized by a short lag phase or its absence and high growth rates (about 1.7 divisions per day) during the exponential phase. Resting cells were formed during the stationary growth phase. Morphological and ultrastructural changes in the cells of A. ussurensis during its life cycle are described based on light and electron microscopic observations. Resting cells retained their photosynthetic activity when stored in the dark for a long period of time (up to 1 year) at a temperature of 4–6°C. After 30, 60, 90, 120, 150, and 180 days of storage under such conditions, the culture of A. ussurensis was capable of regeneration due to the existence of the resting cell stage in the life cycle of the microalga.


Attheya ussurensis morphology ultrastructure resting cells photosynthetic activity 


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  1. 1.
    Andreeva, V.M. and Sedova, T.V., On the Variability of Systematic Characters of Green Unicellular Algae in Culture. 1. The Disappearance of the Pyrenoid, Bot. Zh., 1965, vol. 50, no. 7, pp. 954–961.Google Scholar
  2. 2.
    Bedoshvili, E.D., Popkova, T.P., and Likhoshvai, E.V., The Ultrastructure of the Chloroplasts of Several Diatom Species from Different Classes, Tsitologiya, 2009, vol. 51, no. 4, pp. 346–357.Google Scholar
  3. 3.
    Voronova, E.N., Ilyash, L.V., Pogosyan, S.I., et al., Within-Population Heterogeneity of Fluorescence Parameters in the Marine Planktonic Alga Thalassiosira weisflogii under Different Nitrogen Availability, Mikrobiologiya, 2009, vol. 78, no. 4, pp. 1–10.Google Scholar
  4. 4.
    Gogorev, R.M., Orlova, T.Yu., Shevchenko, O.G., and Stonik, I.V., Diatomovye vodorosli Rosii i sopredel’nykh stran: iskopaemye i sovremennye (Diatoms of Russia and Adjacent Countries: Fossil and Recent), St. Petersburg: Izd. St.-Peterb. Gos. Univ., vol. 2, issue 4, 2006.Google Scholar
  5. 5.
    Orlova, T.Yu. and Aizdaicher, N.A., Development in culture of the Diatom Chaetoceros salsugineus from the Sea of Japan, Russ. J. Mar. Biol., 2000, vol. 26, no. 1, pp. 8–11.CrossRefGoogle Scholar
  6. 6.
    Orlova, T.Yu., Stonik, I.V., and Aizdacher, N.A., Morphology and Biology of the Diatom Attheya longicornis from the Sea of Japan, Russ. J. Mar. Biol., 2002, vol. 28, no. 3, pp. 186–190.CrossRefGoogle Scholar
  7. 7.
    Pechurkin, N.S. and Terskov, I.A., Analiz kinetiki rosta i evolutsii mikrobnykh populyatsii (v upravlyaemykh usloviyakh) [Analysis of Growth Kinetics and Evolution of Microbial Populations (under Controlled Conditions)], Novosibirsk: Nauka, 1975.Google Scholar
  8. 8.
    Pogosyan, S.I., Volkova, E.V., Kazimirko, Yu.V., et al., Changes in the Photosynthetic Apparatus of Individual Cells of the Microalga Ankistrodesmus falcatus in Norm and under UV Irradiation, Dokl. Ross. Akad. Nauk, 1998, vol. 363, no. 5, pp. 690–693.Google Scholar
  9. 9.
    Pogosyan, S.I., Galchuk, S.V., Kazimirko, Yu.V., et al., The Application of a MEGA-25 Fluorimeter to the Estimation of the Quantity of Phytoplankton and the Assessment of the State of the Photosynthetic Apparatus, Voda: Khimiya i Ekologiya, 2009, no. 6, pp. 34–40.Google Scholar
  10. 10.
    Rubin, A.B., Biophysics of Photosynthesis and Methods of Ecological Monitoring, Problemy regulyatsii v biologicheskikh sistemakh, Biofizicheskie aspekty (Problems of Regulation in Biological Systems, Biophysical Aspects), Moscow; Izhevsk: Scientific Research Center for Regular and Chemical Dynamics, Institute of Computer Research, 2007, pp. 424–452.Google Scholar
  11. 11.
    Stonik, I.V., Orlova, T. Yu., and Aizdaicher, N.A., Diatoms of the Genus Attheya West, 1860 from the Sea of Japan, Russ. J. Mar. Biol., 2006, vol. 32, no. 2, pp. 123–126.CrossRefGoogle Scholar
  12. 12.
    Fedorov, V.D., O metodakh izucheniya fitoplanktona i ego aktivnosti (On the Methods for the Study of Phytoplankton and Its Activity), Moscow: Izd. Mosk. Gos. Univ., 1979.Google Scholar
  13. 13.
    Shevchenko, O.G., Orlova, T.Yu., and Aizdaicher, N.A., Development of the Diatom Chaetoceros socialis f. radians (Schütt) Proshkina-Lavrenko 1963 in Laboratory Culture, Russ. J. Mar. Biol., 2008, vol. 34, no. 4, pp. 224–229.CrossRefGoogle Scholar
  14. 14.
    Crawford, R.M., Gardner, C., and Medlin, L.K., The Genus Attheya. 1. A Description of Four New Taxa, and the Transfer of Gonioceros septentrionalis and C. armatus, Diatom Res., 1994, vol. 9, no. 1, pp. 27–51.CrossRefGoogle Scholar
  15. 15.
    Dangeard, P.A., Observations sur une algue cultivée à l’obscurité depuis huit ans, C. R. Acad. Sci. Paris, 1921, vol. 172, pp. 254–260.Google Scholar
  16. 16.
    Eppley, R.W., The Growth and Culture of Diatoms, The Biology of Diatoms, London; Oxford: Blackwell Scientific, 1977, pp. 24–64.Google Scholar
  17. 17.
    Fogg, G.E., Algal Culture and Phytoplankton Ecology, Madison: Univ. of Wisconsin Press, 1966.Google Scholar
  18. 18.
    Genty, B., Briantais, J.-M., and Baker, N.R., The Relationship Between the Quantum Yield of Photosynthetic Electron Transport and Quenching of Chlorophyll Fluorescence, Biochim. Biophys. Acta, 1989, vol. 990,issue 1, pp. 87–92.CrossRefGoogle Scholar
  19. 19.
    Guillard, R.R.L. and Ryther, J.H., Studies of Marine Planktonic Diatoms. 1. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran, Can. J. Microbiol., 1962, vol. 8, no. 2, pp. 229–239.PubMedCrossRefGoogle Scholar
  20. 20.
    Itakura, S., Imai, I., and Iton, K., Morphology and Rejuvenation of Skeletonema costatum (Bacillariophyceae) Resting Cells from the Bottom Sediments of Hiroshima Bay, the Seto Inland Sea, Japan, Bull. Plankton Soc. Jap., 1992, vol. 38, no. 2, pp. 135–145.Google Scholar
  21. 21.
    Kuwata, A., Hama, T., and Takahashi, M., Ecophysiological Characterization of Two Life Forms, Resting Spores and Resting Cells, of a Marine Planktonic Diatom, Chaetoceros pseudocurvisetus, Formed under Nutrient Depletion, Mar. Ecol. Progr. Ser., 1993, vol. 102, pp. 245–255.CrossRefGoogle Scholar
  22. 22.
    Lewin, J. and Schaefer, C.T., The Role of Phytoplankton in Surf Ecosystems, Sandy Beaches as Ecosystems, The Hague: W. Junk Publishers, 1983, pp. 381–389.Google Scholar
  23. 23.
    Lewin, J., Colvin, J.R., and McDonald, K.L., Blooms of Surf-Zone Diatoms along the Coast of the Olympic Peninsula. XXII: The Clay Coat of Chaetoceros armatum T.West, Bot. Mar., 1980, vol. 23, pp. 333–341.Google Scholar
  24. 24.
    Lund, J.W.G., The Seasonal Cycle of the Plankton Melosira italica (Ehr.) Kütz. subsp. subarctica O. Müll., J. Ecol., 1954, vol. 42, no. 1, pp. 151–179.CrossRefGoogle Scholar
  25. 25.
    Nagai, S., Hori, Y., and Manabe, T., Morphology and Rejuvenation of Coscinodiscus wailesii Gran (Bacillariophyceae) Resting Cells Found in Bottom Sediments of Harima-Nada, Seto Inland Sea, Japan, Nippon Suisan Gakk., 1995, vol. 61, pp. 179–185.CrossRefGoogle Scholar
  26. 26.
    Stonik, I.V., Orlova, T.Yu., and Crawford, R.M., Attheya ussurensis sp. nov. (Bacillariophyta) — A New Marine Diatom from the Coastal Waters of the Sea of Japan and a Reappraisal of the Genus, Phycologia, 2006, vol. 45, no. 2, pp. 141–147.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • T. Yu. Orlova
    • 1
  • N. A. Aizdaicher
    • 1
  • I. V. Stonik
    • 1
  • O. G. Schevchenko
    • 1
  • S. I. Pogosyan
    • 2
  1. 1.Zhirmunsky Institute of Marine Biology, Far East DivisionRussian Academy of SciencesVladivostokRussia
  2. 2.Moscow State UniversityMoscowRussia

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