Effects of copper and cadmium ions on the physicochemical properties of lipids of the marine bacterium Pseudomonas putida IB28 at different growth temperatures

  • O. B. Popova
  • N. M. Sanina
  • G. N. Likhatskaya
  • I. P. Bezverbnaya
Biochemistry of Microorganisms

Abstract

The phospholipid and fatty acid composition and thermotropic behavior of total lipids were studied in the metal-accumulating marine strain Pseudomonas putida IB28 grown in the presence of Cu2+ and Cd2+ at 4 and 24°C. Despite the changes in acidic lipid content, unsaturated/saturated fatty acid ratio, and cyclopropane fatty acid level, the temperature range of calorimetric phase transitions of bacterial total lipids was slightly altered under these factors. The suppressive action of heavy metals on bacterial growth is attributable to the phase separation of lipids and, as a consequence, to a sharp increase in the ion permeability of the lipid bilayer. The increase in acidic phospholipid level under the influence of Cu2+ and Cd2+, especially at 24°C, is likely to be indicative of their complexation with heavy metal ions.

Key words

phospholipids fatty acids phase transitions heavy metals Pseudomonas putida 

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • O. B. Popova
    • 1
  • N. M. Sanina
    • 1
  • G. N. Likhatskaya
    • 2
  • I. P. Bezverbnaya
    • 1
  1. 1.Far Eastern State UniversityVladivostokRussia
  2. 2.Pacific Institute of Bioorganic Chemistry, Far East DivisionRussian Academy of SciencesVladivostokRussia

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