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Russian Journal of Developmental Biology

, Volume 49, Issue 6, pp 362–369 | Cite as

Development of Methods and Techniques to Visualize Mechanical Tension in Embryos Using Genetically Encoded Fluorescent Mechanosensors

  • F. M. EroshkinEmail author
  • S. V. Kremnev
  • G. V. Ermakova
  • A. G. Zaraisky
BIOLOGY OF VERTEBRATE DEVELOPMENT

Abstract

Lately, the growing body of quantitative data has provided evidence of the importance of mechanical forces in embryogenesis. The study of spatial and temporal distribution of mechanical tension in the course of embryogenesis is one of the most important problems of modern developmental biology. Development of genetically encoded fluorescent mechanosensors allowed their application in an intravital study of mechanical tension in developing embryos via noninvasive techniques. The possibility of applying fluorescent mechanosensors based on vinculin and C-cadherin to visualize mechanical tension in tissues of Gallus and Xenopus embryos was studied. The methods to express and detect these proteins, as well as process the resulting images, were elaborated. The best results were obtained using Xenopus embryos and the vinculin-based mechanosensor.

Keywords:

Xenopus Gallus embryogenesis mechaniсal tension mechanosensors 

Notes

ACKNOWLEDGMENTS

The work was supported by the Russian Foundation for Basic Research (project no. 15-04-06310). Works on cloning of plasmid constructs were supported by the Russian Science Foundation (project no. 14-50-00131).

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • F. M. Eroshkin
    • 1
    Email author
  • S. V. Kremnev
    • 2
    • 3
  • G. V. Ermakova
    • 1
  • A. G. Zaraisky
    • 1
  1. 1.Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMoscowRussia
  2. 2.Department of Embryology, Faculty of Biology, Moscow State UniversityMoscowRussia
  3. 3.Koltsov Institute of Developmental Biology, Russian Academy of SciencesMoscowRussia

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