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Russian Journal of Developmental Biology

, Volume 46, Issue 4, pp 215–221 | Cite as

In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species

  • D. S. Kulkhanova
  • A. A. Erst
  • T. I. Novikova
Morphogenesis

Abstract

Features of in vitro regeneration of Fritillaria sonnikovae from bulb scales were studied. The initi-ation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 µM 6-benzylami-nopurine and 2 µM α-naphthaleneacetic acid. Optimization of propagation-stage was carried out usingobtained microbulbs as explants. High regenerative response of explants and shoot multiplication rate wereobserved on both media supplemented with growth regulators (up to 47% and 4.2 ± 0.6 shoots per explant,respectively) and hormone-free medium (48% and 4.1 ± 0.2 shoots per explant, respectively). It has beenestablished that the addition of growth regulators on the stage of cultivation does not cause increased mor-phogenic response but contributes to the accelerated initiation and development of microbulbs. Morpholog-ical and histological analysis revealed the dynamics of the formation of shoots de novo. In vitro developmentof F. sonnikovae follows the path of direct organogenesis from the epidermal tissue of the explant.

Keywords

Fritillaria sonnikovae in vitro regeneration morphological and histological analysis bulb scales biodiversity preservation 

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Copyright information

© Pleiades Publishing, Inc. 2015

Authors and Affiliations

  • D. S. Kulkhanova
    • 1
  • A. A. Erst
    • 1
  • T. I. Novikova
    • 1
  1. 1.Central Siberian Botanical Garden, Siberian BranchRussian Academy of SciencesNovosibirskRussia

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