Expression of transmitter receptor genes in early development of sea urchin Paracentrotus lividus
- 56 Downloads
Neurotransmitters (including serotonin and acetylcholine) perform a number of prenervous functions in early sea urchin development. To detect the particular receptor components involved in these processes, we carried out a database search and nucleotide sequences homologous to serotonin receptor type 4, and the α6- and α10-subunits of nicotinic acetylcholine receptor were found among EST-clones from early Paracentrotus lividus embryos. Expression of these transcripts during early development was demonstrated using RT-PCR. These results are the first molecular biology evidence of serotonin and acetylcholine receptor expression in sea urchin early embryogenesis.
Keywordsserotonin receptor nicotinic cholinoreceptor sea urchins early development
Unable to display preview. Download preview PDF.
- Buznikov, G.A., Nizkomolekulyarnye regulyatory zarodyshevogo razvitiya (Low-Molecular-Weight Regulators of Embryonic Development), Moscow: Nauka, 1967.Google Scholar
- Buznikov, G.A. and Podmarev, V.G., Sea Urchins Strondylocentrotus droebachiensis, S. nudus, S. intermedius, in Animal Species for Developmental Studies, Vol. 1: Invertebrates, New York: Consultants Bureau, 1990, pp. 253–285.Google Scholar
- Falugi, C., Diaspro, A., Ramoino, P., Russo, P., and Aluigi, M.G., The Sea Urchin, Paracentrotus lividus, as a Model to Investigate the Onset of Molecules Immunologically Related to the α-7 Subunit of Nicotinic Receptors during Embryonic and Larval Development, Curr. Drug Targets (in press).Google Scholar
- Glennon, R.A., Dukat, M., and Westkaemper, R.B., Serotonin Receptor Subtypes and Ligands, in Psychopharmacology: The Fourth Generation of Progress, Lippincott: Williams and Wilkins, 1995.Google Scholar
- Numanoi, H., Studies on the Fertilization Substances. IV. Presence of Acetylcholine-Like Substance and Cholinesterase in Echinoderm-Germ Cells during Fertilization, in Scient. Papers Coll. Gen. Educ. Univ., Tokyo, 1953, vol. 3, no. 2, pp. 193–200.Google Scholar
- Rostomyan, M.A., Abramyan, K.S., Buznikov, G.A., and Gusareva, E.V., Electronic-Cytochemical Detection of Adenylate Cyclase in Early Sea Urchin Embryos, Tsitologiya, 1985, vol. 27, pp. 877–881.Google Scholar
- Shmukler, Yu.B., Buznikov, G.A., Grigor’ev, N.G., and Mal’chenko, L.A., Effect of Cyclic Nucleotides on the Sensitivity of Early Sea Urchin Embryos to Cytotoxic Neuropharmacological Preparations, Byull. Eksp. Biol. Med., 1984, vol. 97, no. 3, pp. 354–355.Google Scholar
- Shmukler, Yu.B., Grigoriev, N.G., Buznikov, G.A., and Turpaev, T.M., Regulation of Cleavage Divisions: Participation of “Prenervous” Neurotransmitters Coupled with Second Messengers, Comp. Biochem. Physiol., 1986, vol. 83, no. 2, pp. 423–427.Google Scholar