Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal
A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen-coated class slides in serum-free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium chelator BAPTA-AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchronization in MSC consists in calcium-dependent phosphorylation of cell proteins.
Keywordsmesenchymal stromal cells hepatocytes cell interactions circahoralian rhythms protein synthesis rhythm
Unable to display preview. Download preview PDF.
- Harper, N., Hughes, M.A., Farrow, S.N., Cohen, G.M., and MacFarlane, M., Protein Kinase C Modulates Tumor-Necrosis Factor-Related Apoptosis-Inducing Ligand-Induced Apoptosis by Targeting the Apical Events of Death Receptor Signaling, J. Biol. Chem., 2003, vol. 278, pp. 44338–44397.PubMedCrossRefGoogle Scholar