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Russian Journal of Developmental Biology

, Volume 43, Issue 3, pp 196–198 | Cite as

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

  • V. Ya. Brodsky
  • A. V. Vasil’ev
  • V. V. Terskikh
  • N. D. Zvezdina
  • V. I. Fateeva
  • L. A. Mal’chenko
  • E. V. Kiseleva
  • E. I. Bueverova
Short Communication

Abstract

A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen-coated class slides in serum-free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium chelator BAPTA-AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchronization in MSC consists in calcium-dependent phosphorylation of cell proteins.

Keywords

mesenchymal stromal cells hepatocytes cell interactions circahoralian rhythms protein synthesis rhythm 

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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • V. Ya. Brodsky
    • 1
  • A. V. Vasil’ev
    • 1
  • V. V. Terskikh
    • 1
  • N. D. Zvezdina
    • 1
  • V. I. Fateeva
    • 1
  • L. A. Mal’chenko
    • 1
  • E. V. Kiseleva
    • 1
  • E. I. Bueverova
    • 1
  1. 1.Kol’tsov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia

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