Advertisement

Statistical study of rapid mechanodependent cell movements in deformed explants of african clawed frog Xenopus laevis embryonic tissues

  • T. G. Troshina
  • N. S. Glagoleva
  • L. V. Belousov
Biology of Invertebrates

Abstract

Computer analysis of artificially deformed (stretched or compressed) double explants (sandwiches) of the blastocoel roof (BRs) and suprablastoporal region (SBRs) of African clawed frog Xenopus laevis early gastrula has been performed using frames of time-lapse microfilming. During the first 14 min after cutting off, the velocities and displacement angles of several hundreds of cells relative to one another, as well as to fixed points and the extension axis, were measured in the control and deformed samples. It has been found that the deformation of samples leads to a rapid reorientation of large cell masses and increase in the velocities of movements along the extension axes or perpendicularly to the compression axes. In addition, an increase in the velocities of mutual cell displacements in the stretched BRs and cell convergence to the extension axes have been observed. Comparison of different angular sectors demonstrates a statistically significant positive correlation between the mean velocities of cell movements and the number of cells moving within an individual sector. This suggests cooperativity of mechanodependent cell movements. In general, these results demonstrate an important role of mechanical factors in regulation of collective cell movements.

Keywords

cell movements mechanical tensions cooperativity Xenopus laevis gastrula 

References

  1. Beloussov, L.V., Louchinskaia, N.N., and Stein, A.A., Tension-Dependent Collective Cell Movements in the Early Gastrula Ectoderm of Xenopus laevis Embryos, Dev. Genes Evol., 2000, vol. 210, pp. 92–104.PubMedCrossRefGoogle Scholar
  2. Glagoleva, N.S., Beloussov, L.V., Shtein, A.A., and Luchinskaya, N.N., A Quantitative Study of Regional and Stage Specific Reaction of Xenopus laevis Embryonic Tissues on Mechanical Load, Russ. J. Dev. Biol., 2003, vol. 34, pp. 241–248.CrossRefGoogle Scholar
  3. Keller, R., Shook, D., and Skoglund, P., The Forces that Shape Embryos: Physical Aspects of Convergent Extension by Cell Intercalation, Phys. Biol., 2008, vol. 5, no. 1, p. 015007.PubMedCrossRefGoogle Scholar
  4. Keller, R.L., Davidson, A., Edlund, T., Elul, M., Shook, D., and Skoglund, P., Mechanisms of Convergence and Extension by Cell Intercalation, Philos. Trans. R. Soc. Lond. B Biol. Sci., 2000, vol. 355, pp. 897–922.PubMedCrossRefGoogle Scholar
  5. Kornikova, E.S., Korvin-Pavlovskaya, E.G., and Beloussov, L.V., Relocations of Cell Convergence Sites and Formation of Pharyngula-Like Shapes in Mechanically Relaxed Xenopus Embryos, Dev. Genes Evol., 2009, vol. 219, pp. 1–10.PubMedCrossRefGoogle Scholar
  6. Nieuwkoop, P.D. and Faber, J., Normal Table of Xenopus laevis (Daudin), North-Holland Publ., 1956.Google Scholar
  7. Tahinci, E. and Symes, K., Distinct Functions of Rho and Rac Are Required for Convergent Extension during Xenopus Gastrulation, Dev. Biol., 2003, vol. 15, no. 259 (2), pp. 318–335.CrossRefGoogle Scholar
  8. Wallingford, J.B., Fraser, S.E., and Harland, R.M., Convergent Extension: the Molecular Control of Polarized Cell Movement during Embryonic Development, Dev. Cell., 2002, vol. 2, no. 6, pp. 695–706.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • T. G. Troshina
    • 1
  • N. S. Glagoleva
    • 1
  • L. V. Belousov
    • 1
  1. 1.Moscow State UniversityMoscowRussia

Personalised recommendations