Survival of transgenic mouse embryos carrying different genetic constructs
The survival of transgenic mouse embryos was studied as a function of the transgene structure. The data obtained indicate that the introduction of a chromosomal DNA fragment providing for the anchoring of interphase chromosomes on the nuclear envelope increases the efficiency of transgenesis in mice threefold due to their increased viability.
Key wordstransgenesis embryonic viability compartmentalization of genes in the nucleus
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- Brem, G, Transgenic animals, in Biotechnology. V. 2. Genetic Fundamentals and Engineering, Ed. Puhler A. et al., Eds., New York: Wiley-VCH, 1993, pp. 745–832.Google Scholar
- Glazkov, M.V., Chromosome Association with the Nuclear Envelope and the Orderliness of the Spatial Organization of the Genetic Material in the Interphase Nucleus, Tsitol. Genet., 1999, vol. 2, no. 2, pp. 79–88.Google Scholar
- Glazkov, M.V., Boundaries of Structure-Functional Units (Domains) of Eukaryotic Chromosomes, Genetika, 1998, vol. 32, no. 5, pp. 593–604.Google Scholar
- Glazkov, M.V., Kleimans, A., and Kireeva, N.N., Specificity of DNA-Protein Interactions during Formation of Rosettelike Structures (Elementary Chromomeres) and during Association of Interphase Chromosomes with the Nuclear Envelope, Mol. Biol., 1998, vol. 32, no. 5, pp. 869–874.Google Scholar
- Hogan, B., Constantini, F., and Lacy, E., Manipulating the Mouse Embryo, New York: Cold Spring Harbor Lab., 1986.Google Scholar