Advertisement

Cryopreservation of Drosophila or How to Save on Collection Maintenance

  • 45 Accesses

Abstract

An analysis of the literature on the cryopreservation of Drosophila melanogaster, the collections of which include hundreds of thousands of lines, was conducted. It was noted that the cryopreservation of D. melanogaster embryos is possible only at the 14th stage of development after permeabilization of the chorion, which prevents the penetration of cryoprotectants, and succeeds in 3% of cases. The successful cryopreservation of Drosophila larvae and imago was carried out so far only for the Chymomyza costata species. It was established that it is possible to save the cost of maintaining the collection of D. melanogaster by extending ontogenesis due to reducing the feed calorie content and the temperature, as well as due to shortening of the light period.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

REFERENCES

  1. 1

    Colinet, H. and Renault, D., Dietary live yeast alters metabolic profiles, protein biosynthesis and thermal stress tolerance of Drosophila melanogaster. Part A, Comp. Biochem. Phys., 2014, vol. 170, pp. 6–14.

  2. 2

    Jakobs, R., Ahmadi, B., Houben, S., Gariepy, T.D., and Sinclair, B.J., Cold tolerance of third-instar Drosophila suzukii larvae, J. Insect Physiol., 2017, vol. 96, pp. 45–52. https://doi.org/10.1016/j.jinsphys.2016.10.008

  3. 3

    Kostál, V., Zahradnícková, H., and Šimek, P., Hyperprolinemic larvae of the drosophilid fly. Chymomyza costata survive cryopreservation in liquid nitrogen, Proc. Natl. Acad. Sci. U. S. A., 2011, vol. 108, pp. 13041–13046. https://doi.org/10.1073/pnas.1107060108

  4. 4

    Koštál, V., Korbelová, J., Poupardin, R., Moos, M., and Šimek, P., Arginine and proline applied as food additives stimulate high freeze tolerance in larvae of Drosophila melanogaster,J. Exp. Biol., 2016, vol. 219, pt 15, pp. 2358–2367. https://doi.org/10.1242/jeb.142158

  5. 5

    Lakovaara, S., Saura, A., Koref-Santibanez, S., and Ehrman, L., Aspects of diapause and its genetics in northern drosophilids, Hereditas, 1972, vol. 70, pp. 89–96.

  6. 6

    Lynch, D.V., Lin, T.T., Myers, S.P., Leibo, S.P., Macintyre, R.J., Pitt, R.E., and Steponkus, P.L., A two-step method for permeabilization of Drosophila eggs, Cryobiology, 1989, vol. 26, pp. 445–452.

  7. 7

    MacMillan, H.A., Knee, J.M., Dennis, A.B., Udaka, H., Marshall, K.E., Merritt, T.J., and Sinclair, B.J., Sci. Rep., vol. 6, p. 28999. https://doi.org/10.1038/srep28999

  8. 8

    Mazur, P., Cryobiology: the freezing of biological systems, Science, 1970, vol. 168, pp. 939–949. https://doi.org/10.1126/science.168.3934.939

  9. 9

    Mazur, P., Cole, K.W., Hall, J.W., Schreuders, P.D., and Mahowald, A.P., Cryobiological preservation of Drosophila embryos, Science, 1992b, vol. 258, pp. 1932–1935.

  10. 10

    Mazur, P., Cole, K.W., and Mahowald, A.P., Critical factors affecting the permeabilization of Drosophila embryos by alkanes, Cryobiology, 1992a, vol. 29, pp. 210–239.

  11. 11

    Mazur, P., Cole, K.W., Schreuders, P.D., and Mahowald, A.P., Contributions of cooling and warming rate and developmental stage to the survival of Drosophila embryos cooled to –205 degrees C, Cryobiology, 1993, vol. 30, pp. 45–73. https://doi.org/10.1006/cryo.1993.1006

  12. 12

    Myers, S.P., Pitt, R.E., Lynch, D.V., and Steponkus, P.L., Characterization of intracellular ice formation in Drosophila melanogaster embryos, Cryobiology, 1989, vol. 26, pp. 472–484.

  13. 13

    Piper, M.D.W. and Partridge, L., Dietary restriction in drosophila: delayed aging or experimental artefact?, PLoS Genet., 2007, vol. 3, no. 4. e570461.

  14. 14

    Riggs, R., Mayer, J., Dowling-Lacey, D., Chi, T.F., Jones, E., and Oehninger, S., Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos, Fertil. Steril., 2008, vol. 93, pp. 109–115. https://doi.org/10.1016/j.fertnstert.2008.09.084

  15. 15

    Steponkus, P.L., Myers, S.P., Lynch, D.V., Gardner, L., Bronshteyn, V., Leibo, S.P., Rall, W.F., Pitt, R.E., Lin, T.T., and MacIntyre, R.J., Cryopreservation of Drosophila melanogaster embryos, Nature, 1990, vol. 345, pp. 170–172.

  16. 16

    Yancey, P.H. and Siebenaller, J.F., Co-evolution of proteins and solutions: protein adaptation versus cytoprotective micromolecules and their roles in marine organisms, J. Exp. Biol., 2015, vol. 218, pt 12, pp. 1880–1896. https://doi.org/10.1242/jeb.114355

  17. 17

    Yancey, P.H., Clark, M.E., Hand, S., Bowlus, R.D., and Somero, G.N., Living with water stress: evolution of osmolyte systems, Science, 1982, vol. 217, pp. 1214–1222.

  18. 18

    Zonato, V., Collins, L., Pegoraro, M., Tauber, E., and Kyriacou, C.P., Is diapause an ancient adaptation in drosophila?, J. Insect Physiol., 2017, vol. 98, pp. 267–274. https://doi.org/10.1016/j.jinsphys.2017.01.017

Download references

Author information

Correspondence to L. P. Zakharenko.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by A. Barkhash

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zakharenko, L.P. Cryopreservation of Drosophila or How to Save on Collection Maintenance. Biol Bull Russ Acad Sci 46, 544–546 (2019). https://doi.org/10.1134/S1062359019060153

Download citation