Advertisement

Biology Bulletin

, Volume 46, Issue 6, pp 577–582 | Cite as

Effects of Limonium effusum Ethanol Extracts on Cell Proliferation and Mutagenicity

  • Yasin ErenEmail author
BOTANY
  • 10 Downloads

AbstractLimonium plants have commonly used as antibacterial, styptical, anti-swelling agent, digestive system regulator, anticarcinogenic, and antiviral in alternative medicine. Beside this, different extracts of Limonium plants have mutagenic, cytotoxic and genotoxic effects. Different parts of Limonium effusum (Boiss.) Kuntze ethanol extracts were used to determine the mutagenic and cytotoxic effects. Ames test was carried out with Salmonella typhimurium TA98, and TA100 strains to determine the mutagenicity. Ames test results showed that root 1000 μg/plate of plant has mutagenic effect and root extracts have weak mutagenicity with TA98 strain. MTT test was carried out with MDBK (Madin-Darby Bovine Kidney) cells to determine the cytotoxicity. Cytotoxicity results showed that root 50, 25, 12.5 and 6.25 μg/mL extracts were increased the proliferation rates in 24 h treatment. All doses of root extracts decreased the rates in 96 h. All concentrations of stem decreased the proliferation rates in 96 h. Leaf extracts increased the rates in 24 h but decreased in 48, 72, and 96 h.

Keywords: Limonium effusum, MTT test Ames test mutagenicity cytotoxicity 

Notes

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

REFERENCES

  1. 1.
    Ali, N.A.A., Mothana, R., Ghaleb, N., and Lindequist, U., Screening of traditionally used endemic Soqotraen plants for cytotoxic activity, Afr. J. Trad. CAM., 2007, vol. 4, pp. 529–531.CrossRefGoogle Scholar
  2. 2.
    Avaz, S., Korcan, S.E., Kargioglu, M., Serteser, A., Konuk, M., and Erol, I., Antimicrobial activities in root extracts of Limonium spp. growing in Afyonkarahisar, Turkey, J. Environ. Biol., 2013, vol. 34, pp. 561–568.PubMedGoogle Scholar
  3. 3.
    Barnes, W.S. and Klekowski, E.J., Testing the environment for dispersed mutagens: use of plant bioconcentrators coupled with microbial mutagen assays, Environ. Health Persp., 1978, vol. 27, pp. 61–67.Google Scholar
  4. 4.
    Başaran, A.A., Yu, T.W., Plewa, M.J., and Anderson, D., An investigation of some Turkish herbal medicines in Salmonella typhimurium and the Comet assay in human lymphocytes, Teratog. Carcinog. Mutagen., 1996, vol. 16, pp. 125–138.CrossRefGoogle Scholar
  5. 5.
    Boldrin, P.K., Resende, F.A., Höhne, A.P.O., Camargo, M.S., Espanha, L.G., Nogueira, C.H., Melo, M.S.F., Vilegas, W., and Varanda, E.A., Estrogenic and mutagenic activities of Crotalaria pallida measured by recombinant yeast assay and Ames test, BMC Complement. Altern. Med., 2013, vol. 13, p. 216.CrossRefGoogle Scholar
  6. 6.
    Davis, P.H., Mill, R.R., and Tan, K., Flora of Turkey and the East Aegean Islands, Edinburgh: Edinburgh Univ. Press, 1982, vol. 7, pp. 186–187.Google Scholar
  7. 7.
    Dean, B.J., Brooks, T.M., Hodson-Walker, G., and Hutson, D.H., Genetic toxicology testing of 41 industrial chemicals, Mutat. Res., 1985, vol. 153, pp. 57–77.CrossRefGoogle Scholar
  8. 8.
    Demma, J., Engidawork, E., and Hellman, B., Potential genotoxicity of plant extracts used in Ethiopian traditional medicine, J. Ethnopharm., 2009, vol. 122, no. 1, pp. 136–142.CrossRefGoogle Scholar
  9. 9.
    Eren, Y., Mutagenic and cytotoxic activities of Limonium globuliferum methanol extracts, Cytotechnology, 2016, vol. 68, pp. 2115–2124.CrossRefGoogle Scholar
  10. 10.
    Eren, Y. and Özata, A., Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests, Rev. Bras. Pharmacogn., 2014, vol. 24, pp. 51–59.CrossRefGoogle Scholar
  11. 11.
    Eren, Y., Özata, A., Konuk, M., Akyil, D., and Liman, R., A Mutagenicity and cytotoxicity study on Limonium effusum aqueous extracts by Allium, Ames and MTT tests, Cytol. Genet., 2015, vol. 49, no. 2, pp. 125–133.CrossRefGoogle Scholar
  12. 12.
    Espanha, L.G., Resende, F.A., Neto, J.S.L., Boldrin, P.K., Nogueira, C.H., Camargo, M.S., De Grandis, R.A., Santos, L.C., Vilegas, W., and Varanda, E.A., Mutagenicity and antimutagenicity of six Brazilian Byrsonima species assessed by the Ames test, BMC Complement. Altern. Med., 2014, vol. 14, p. 182.CrossRefGoogle Scholar
  13. 13.
    De Sá Ferreira, I.C.F. and Vargas, V.M., Mutagenicity of medicinal plant extracts in Salmonella/microsome assay, Phyto.Res., 1999, vol. 13, pp. 397–400.Google Scholar
  14. 14.
    Ksouri, R., Meghdiche, W., Jalleli, I., Debez, A., Magné, C., Hiroco, I., and Abdelly, C., Medicinal halophytes: potent source of health promoting biomolecules with medical, nutriceutical and food applications, Crit. Rev. Biotechnol., 2012, pp. 1–38.Google Scholar
  15. 15.
    Maron, D.M. and Ames, B.N., Revised methods for the Salmonella mutagenicity test, Mutat. Res., 1983, vol. 113, pp. 173–215.CrossRefGoogle Scholar
  16. 16.
    Mortelmans, K. and Zeiger, E., The Ames Salmonella/microsome mutagenicity assay, Mutat. Res., 2000, vol. 455, pp. 29–60.CrossRefGoogle Scholar
  17. 17.
    Mosmann, T., Rapid colonmetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods, 1983, vol. 65, pp. 55–63.CrossRefGoogle Scholar
  18. 18.
    Rastogi, S., Pandey, M.M., and Rawat, A.K.S., Traditional herbs: a remedy for cardiovascular disorders, Phytomedicine, 2016, vol. 23, pp. 1082–1089.CrossRefGoogle Scholar
  19. 19.
    Romero-Jimenez, J., Campos-Sanchez, M., Allana, A., Munoz-Serrano, A., and Alonso, M., Genotoxicity and antigenotoxicity of some traditional medicinal herbs, Mutat. Res., vol. 585, pp. 147–155.CrossRefGoogle Scholar
  20. 20.
    Seo, Y., Lee, H., Ah, KimY., Youn, H.J., and Lee, B., Effects of several salt marsh plants on mouse spleen and thymus cell proliferation using MTT assay, Ocean Sci. J., 2005, vol. 40, pp. 209–212.CrossRefGoogle Scholar
  21. 21.
    Singh, N.P., McCoy, T.M., Tice, R.R., and Schneider, L.E., A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 1988, vol. 175, no. 1, pp. 184–191.CrossRefGoogle Scholar
  22. 22.
    Sirohi, S.K., Pandey, N., Goel, N., Singh, B., Mohini, M., Pandey, P., and Chaudhry, P.P., Microbial activity and ruminal methanogenesis as affected by plant secondary metabolites in different plant extracts, Int. J. Civil.Environ. Eng., 2009, vol. 1, pp. 52–58.Google Scholar
  23. 23.
    Sohni, Y.R., Mutangadura-Mhlanga, T., and Kale, P.G., Bacterial mutagenicity of eight medicinal herbs from Zimbabwe, Mutat. Res., 1994, vol. 322, pp. 133–140.CrossRefGoogle Scholar
  24. 24.
    Tang, X.H., Yu, F., Liu, J., Gao, J., Yan, L.F., and Dong, M.M., Isolation and identification of anti-tumor polysaccharide LSP21 from Limonium sinense (Girard) Kuntze, Int. J. Biol. Macromol., vol. 70, pp. 138–142.CrossRefGoogle Scholar
  25. 25.
    Yang, E., Yim, E., Song, G., Kim, G., and Hyun, C., Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages by Jeju plant extracts, Interdisc. Toxicol., 2009, vol. 2, pp. 245–249.CrossRefGoogle Scholar
  26. 26.
    Zhang, T., Zhao, Q., Zhang, Y., and Ning, J., Assessment of genotoxic effects of flumorph by the comet assay in mice organs, Hum. Exp. Toxicol., 2014, vol. 33, pp. 224–229.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  1. 1.Department of Science Education, Faculty of Education, Süleyman Demirel UniversityIspartaTurkey

Personalised recommendations