Biology Bulletin

, Volume 46, Issue 6, pp 525–530 | Cite as

Melafen Alleviates the Harmful Effect of Salt Stress on Calendula Plants and Accelerates Their Recovery

  • L. A. Stetsenko
  • Vl. V. KuznetsovEmail author


It has been shown for the first time that 10–9 M melafen boosted the plant tolerance of the marigold (Calendula officinalis L.) to salt stress and accelerated plant recovery occurring after removal of the excess salt from the growing medium. During both salt stress and its recovery, melafen attenuated the inhibitory effect of salt on biomass accumulation and the leaf assimilating area, stabilized the water status, and lowered the concentrations of malondialdehyde and stress-induced proline but did not affect the contents of the main photosynthetic pigments.



The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.


  1. 1.
    Bates, L.S., Waldren, R.P., and Teare, I.D., Rapid determination of free proline for water-stress studies, Plant Soil, 1973, vol. 39, pp. 205–207.CrossRefGoogle Scholar
  2. 2.
    Binyukov, V.I., Mil’, E.M., Zhigacheva, I.V., Albantova, A.A., Generozova, I.P., Shugaev, A.G., Fattakhov, S.G., and Konovalov, A.I., The combination of moisture deficit, moderate cooling, and melafen changes the morphology of mitochondria in pea seedling, Dokl. Biochem. Biophys., 2012, vol. 446, pp. 220–222.CrossRefGoogle Scholar
  3. 3.
    Fattakhov, S.G., Loseva, N.L., and Reznik, B.C., Melamine salt of bis-(hydroxymethyl) phosphinic acid (melafen) as a regulator of growth and development of plants and a method of its obtaining, RF Patent No. 2158735, 2000.Google Scholar
  4. 4.
    Fattakhov, S.G., Kuznetsov, V.V., and Zagoskina, N.V., Melafen: mekhanizm deistviya i oblasti primeneniya (Melaphen: Mechanism of Action and Application Fields), Kazan, 2014.Google Scholar
  5. 5.
    Heath, R.L. and Packer, L., Photoperoxidation in isolated chloroplasts. Kinetics and stoichiometry of fatty acid peroxidation, Arch. Biochem. Biophys., 1968, vol. 125, рр. 189–198.CrossRefGoogle Scholar
  6. 6.
    Kuznetsov, Vl.V. and Shevyakova, N.I., Proline under stress: biological role, metabolism, and regulation, Russ. J. Plant Physiol., 1999, vol. 46, vol. 2, pp. 284–288.Google Scholar
  7. 7.
    Kuznetsov, V.V., Kravtsov, A.K., Selivankina, S.Yu., Zubo, Ya.O., Zubkova, N.K., Kulaeva, O.N., Fattakhov, S.G., and Konovalov, A.I., Melafen stimulates RNA polymerase I activity but has no effect on plastid gene transcription in barley, Dokl. Biochem. Biophys., 2010, vol. 431, pp. 82–86.CrossRefGoogle Scholar
  8. 8.
    Ladyzhenskaya, A.P., Platonov, T.A., Evsyunina, A.S., Fattakhov, S.G., Korableva, N.P., and Reznik, V.S., The effect of melamine salt of bis(oxymethyl)phosphinic acid (melafen) on the growth processes and plasma membrane function in potato tuber cells, Appl. Biochem. Microbiol., 2007, vol. 43, pp. 222–226.CrossRefGoogle Scholar
  9. 9.
    Latysheva, N.V. and Usova, K.A., Higher yields of calendula as a result of application of fertilizers and EPIN-EXTRA preparation, Nov. Nauka: Opyt, Tradits., Innovats., 2016, no. 591, pt. 2, pp. 14–16.Google Scholar
  10. 10.
    Lichtenthaler, H.K., Chlorophylls and carotenoids, pigments of photosynthetic biomembranes, Methods Enzymol., 1987, vol. 148, pp. 350–382.CrossRefGoogle Scholar
  11. 11.
    Strogonov, B.P., Metabolizm rastenii v usloviyakh zasoleniya (The Metabolism of Plants Under Salination Conditions), Moscow: Nauka, 1973.Google Scholar
  12. 12.
    Zagoskina, N.V., Pinaev, A.S., Alyavina, A.K., Yamburenko, M.V., Gladyshko, T.O., Kuznetsov, V.V., and Konovalov, A.I., Activation of growth and accumulation of phenolic compounds in tea callus culture by melafen is not associated with its possible cytokinin activity, Dokl. Biochem. Biophys., 2007, vol. 413, pp. 88–91.CrossRefGoogle Scholar
  13. 13.
    Zhigacheva, I.V., Fatkullina, L.D., Rusina, I.F., Shugaev, A.G., Generozova, I.P., Fattakhov, S.G., and Konovalov, A.I., Antistress properties of melafen, Dokl. Biochem. Biophys., 2007a, vol. 414, pp. 106–108.CrossRefGoogle Scholar
  14. 14.
    Zhigacheva, I.V., Fatkullina, L.D., Shugaev, A.G., Generozova, I.P., Fattakhov, S.G., Reznik, V.S., and Konovalov, A.I., Effect of melafen on functional efficiency of mitochondrial membranes from sugar beet taproots, Russ. J. Plant Physiol., 2007b, vol. 54, no. 5, pp. 595–599.CrossRefGoogle Scholar
  15. 15.
    Zhigacheva, I.V., Burlakova, E.B., and Goloshchapov, A.N., The functional state of mitochondria determines the tolerance of pea seedlings to insufficient moisture, Akt. Vopr. Biol. Fiz. Khim., 2016, nos. 1–2, pp. 168–172.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  1. 1.Timiryazev Institute of Plant Physiology, Russian Academy of SciencesMoscowRussia

Personalised recommendations