Advertisement

Changes in Prooxidant-Antioxidant System Indices in the Blood and Brain of Rats with Modelled Acute Hypoxia which Consumed a Deuterium-Depleted Drinking Diet

Abstract

Using the rat hypoxia model, the imbalanced work of antiradical defense enzymes with insufficient dismutase activity in blood leading to the development of oxidative stress, which was more pronounced in animals receiving a natural drinking diet, has been confirmed on the basis of the comparative analysis of the antiradical defense enzyme functioning index. It has been observed that acute hypoxia in the brain tissues is characterized by the development of catalase deficiency and the risk of excessive hydrogen peroxide production. It has been demonstrated that a decrease in the D/H (deuterium/protium) ratio in the blood and brain reduces the severity of the impairment of antioxidant enzyme work in hypoxia.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

REFERENCES

  1. 1

    Bayliak, M., Gospodaryov, D., Semchyshyn, G., and Lushchak, V., Inhibition of catalase by aminotriazole in vivo results in reduction of glucose-6-phosphate dehydrogenase activity in Saccharomyces cerevisiae cells, Biochemistry (Moscow), 2008, vol. 73, no. 4, pp. 420–426.

  2. 2

    Basov, A.A., Bykov, I.M., Gubareva, E.A., Pavlyuchenko, I.I., and Fedosov, S.R., A method for diagnosing metabolic disorders in the body under conditions of oxidative stress, RF Patent No. 2436101, Byull. Izobret., 2011, no. 34.

  3. 3

    Beers, R. and Sizer, I., A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase, J. Biol. Chem., 1952, vol. 195, pp. 133–140.

  4. 4

    Bila, W.C., Mariano, R.M.S., Silva, V.R., Santos, M.E.M., Lamounier, J.A., Ferriolli, E., and Galdino, A.S., Applications of deuterium oxide in human health, Isotopes Environ. Health Stud., 2017, vol. 53, no. 4, pp. 327–343.

  5. 5

    Dzhimak, S.S., Basov, A.A., Fedulova, L.V., Didikin, A.S., Bikov, I.M., Arcybasheva, O.M., Naumov, G.N., and Baryshev, M.G., Correction of metabolic processes in rats during chronic endotoxicosis using isotope (D/H) exchange reactions, Biol. Bull. (Moscow), 2015a, vol. 42, no. 5, pp. 440–448.

  6. 6

    Dzhimak, S.S., Basov, A.A., Kopytov, G.F., Kashaev, D.V., Sokolov, M.E., Artsybasheva, O.M., Sharapov, K.S., and Baryshev, M.G., Application of NMR spectroscopy to the determination of low concentrations of nonradioactive isotopes in liquid media, Russ. Phys. J, 2015b, vol. 58, no. 7, pp. 923–929.

  7. 7

    Dzhimak, S.S., Basov, A.A., Volchenko, N.N., Samkov, A.A., Fedulova, L.V., and Baryshev, M.G., Changes in the functional activity of mitochondria isolated from the liver of rat that passed the preadaptation to ultra-low deuterium concentration, Dokl. Biochem. Biophys., 2017, vol. 476, pp. 323–325.

  8. 8

    Esmaeelpanah, E., Rahmatkhah, A., Poormahmood, N., Razavi, B.M., Hasani, F.V., and Hosseinzadeh, H., Protective effect of green tea aqueous extract on acrylamide induced neurotoxicity, Jundishapur J. Nat. Pharm. Prod., 2015, vol. 10, no. 2. e18406.

  9. 9

    Fedulova, L.V., Vasilevskaya, E.R., Kotenkova, E.A., Elkina, A.A., Baryshev, M.G., and Lisitsyn, A.B., Influence of different polypeptides fractions derived from Sus scrofa immune organs on the rats immunological reactivity, J. Pharm. Nutrition Sci., 2017, vol. 7, no. 2, pp. 35–40.

  10. 10

    Frolov, V.Yu., Baryshev, M.G., Bolotin, S.N., and Dzhimak, S.S., A method of producing biologically active deuterium-depleted drinking water, RF Patent No. 2438765, Byull. Izobret., 2012, no. 1.

  11. 11

    Kostyuk, B.A., Potapovich, A.I., and Kovaleva, Zh.I., A simple and sensitive method for determining the activity of superoxide dismutase based on the quercetin oxidation reaction, Vopr. Med. Khim., 1990, vol. 36, no. 2, pp. 88–91.

  12. 12

    Mehri, S., Dadesh, Q., Tabeshpour, J., Hassani, F.V., Karimi, G., and Hosseinzadeh, H., Evaluation of the neuroprotective effect of silymarin on acrylamide-induced neurotoxicity, Jundishapur J. Nat. Pharm. Prod., 2016, vol. 11, no. 4. e37644.

  13. 13

    Mladin, C., Ciobica, A., Lefter, R., Popescu, A., and Bild, W., Deuterium-depleted water has stimulating effects on long-term memory in rats, Neurosci. Lett., 2014, vol. 583, pp. 154–158.

  14. 14

    Rasooli, A., Fatemi, F., Akbarzadeh, K., Dini, S., and Bahremand, S., Synergistic protective activity of deuterium depleted water (DDW) and Satureja rechingeri essential oil on hepatic oxidative injuries induced by acetaminophen in rats, J. Essential Oil Bearing Plants, 2016, vol. 19, no. 5, pp. 1086–1101.

  15. 15

    Rehakova, R., Klimentova, J., Cebova, M., Barta, A., Matuskova, Z., Kreber, Labaš P., and Pechanova, O., Effect of deuterium-depleted water on selected cardiometabolic parameters in fructose-treated rats, Physiol. Res., 2016, vol. 65, no. 3, pp. 401–S407.

  16. 16

    Shurygina, L.V., Zlishcheva, E.I., Khablyuk, V.V., Kravtsova, A.N., Abramova, N.O., Zlishcheva, L.I., and Kravtsov, A.A., A comparative study of antioxidant properties of comenic acid and potassium comenate in an immobilization stress model, Byull. Eksp. Biol. Med., 2015, vol. 159, no. 4, pp. 457–459.

  17. 17

    Strekalova, T., Evans, M., Chernopiatko, A., Couch, Y., Costa-Nunes, J., Cespuglio, R., Chesson, L., Vignisse, J., Steinbusch, H.W., Anthony, D.C., Pomytkin, I., and Lesch, K.P., Deuterium content of water increases depression susceptibility: the potential role of a serotonin-related mechanism, Behav. Brain Res., 2015, vol. 277, pp. 237–244.

  18. 18

    Vladimirov, Yu.A. and Proskurina, E.V., Free radicals and cell chemiluminescence, Usp. Biol. Khim., 2009, vol. 49, pp. 341–388.

  19. 19

    Zrelov, O.Y., Syroeshkin, A.V., Uspenskaya, E.V., Titorovich, O.V., and Pleteneva, T.V., Effect of water isotopic composition on galactose mutarotation kinetics, Pharm. Chem. J., 2015, vol. 49, no. 6, pp. 413–416.

Download references

Author information

Correspondence to A. A. Basov.

Ethics declarations

Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Additional information

Translated by E. Martynova

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Basov, A.A., Kozin, S.V., Bikov, I.M. et al. Changes in Prooxidant-Antioxidant System Indices in the Blood and Brain of Rats with Modelled Acute Hypoxia which Consumed a Deuterium-Depleted Drinking Diet. Biol Bull Russ Acad Sci 46, 531–535 (2019). https://doi.org/10.1134/S1062359019060049

Download citation