Preconcentration of Catecholamins on Hypercrosslinked Polystyrene and Their Determination by High-Performance Liquid Chromatography
The use of hypercrosslinked polystyrene for the group sorption preconcentration of catecholamines is proposed. The conditions for the preconcentration of noradrenaline, adrenaline, and dopamine are optimized: a microcolumn (10 × 6 mm), mass of adsorbent 0.03 g, 25 mL of solution (pH ~ 8.5), and transmission rate of the solution 1.0 mL/min. Compounds were desorbed with 1 mL of 6 M acetic acid and determined in the eluate by reversed-phase HPLC with an amperometric detector (E = 0.8 V). Adsorption preconcentration ensures the reduction of the limits of detection for catecholamines by more than 20 times. The limits of detection were 0.7 ng/mL for dopamine and 1 ng/mL for noradrenaline and adrenaline. The procedure was applied to the analysis of model mixtures based on urine.
Keywords:adrenaline noradrenaline dopamine solid phase extraction hypercrosslinked polystyrene HPLC
The work was performed with the financial support of the Russian Science Foundation, project no. 18-73-10001 using equipment acquired from the funds of the Moscow University Development Program.
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