Journal of Analytical Chemistry

, Volume 74, Issue 1, pp 81–86 | Cite as

Extraction and Spectrophotometric Determination of Molybdenum(VI) using 3-hydroxy-2-[3-(4-methoxyphenyl)-1-phenyl-4-pyrazolyl]-4-oxo-4H-1-benzopyran as a Chelating Agent

  • R. Agnihotri
  • A. Singh
  • N. AgnihotriEmail author


An extraction-spectrophotometric method has been developed for the determination of trace molybdenum(VI) using a newly synthesized benzopyran derivative, 3-hydroxy-2-[3-(4-methoxyphenyl)-1-phenyl-4-pyrazolyl]-4-oxo-4H-1-benzopyran (HMPPB). The method involves formation of a 1 : 2 (M : L) yellow complex between Mo(VI) and HMPPB from 0.02–0.06 M H2SO4 medium. The Mo(VI)–HMPPB complex is easily extractable into carbon tetrachloride with 100% extraction and exhibits maximum absorbance in the wavelength region 417–423 nm. The method is made sensitive and selective to produce best extraction results by studying and optimizing parameters of acidity, reagent concentration, equilibration time and effect of diverse ions. The optimized method shows a good range of linearity (0–1.4 µg/mL), sensitivity (0.0018 µg/cm2), limit of detection (0.1 µg/mL), correlation coefficient (0.9974), accuracy, and reproducibility (±0.002 absorbance units). The proposed method is satisfactorily applied to the extraction and determination of molybdenum(VI) from different synthetic and technical samples including steel, flue dust and water samples.


molybdenum 3-hydroxy-2-[3-(4-methoxyphenyl)-1-phenyl-4-pyrazolyl]-4-oxo-4H-1-benzopyran solvent extraction spectrophotometry determination 



Thanks are due to the Authorities, Maharishi Markandeshwar University, Mullana and Kurukshetra University, Kurukshetra for providing laboratory and other desired facilities.


  1. 1.
    Cotton, F.A., Wilkinson, G., Murillo, C.A., and Bochman, M., Advanced Inorganic Chemistry, New York: Wiley, 1999, 6th ed.Google Scholar
  2. 2.
    Jones, U.S., Fertilizers and Soil Fertility, New Delhi: Prentice Hall, 1987, 2nd ed.Google Scholar
  3. 3.
    Marczenko, Z. and Lobinski, R., Pure Appl. Chem., 1991, vol. 63, p. 1627.CrossRefGoogle Scholar
  4. 4.
    Curzon, M.E.J., Kubota, J., and Bibby, B.G., J. Dent. Res., 1971, vol. 50, p. 74.CrossRefGoogle Scholar
  5. 5.
    Agnihotri, R., Akhtar, S., Singh, A., and Agnihotri, N., Res. Rev.: J. Chem., 2016, vol. 5, p. 22.Google Scholar
  6. 6.
    Sandell, E.B., Colorimetric Determination of Traces of Metals, New York: Interscience, 1959.Google Scholar
  7. 7.
    Dass, R., Kumar, A., and Sharma, R.G., Indian J. Chem., Sect. A: Inorg., Bio-Inorg., Phys., Theor. Anal. Chem., 2004, vol. 43, p. 576.Google Scholar
  8. 8.
    Kania, K. and Buhl, F., Chem. Anal. (Warsaw), 1993, vol. 38, p. 613.Google Scholar
  9. 9.
    Ahmed, M.J. and Haque, M.E., Anal. Sci., 2002, vol. 18, p. 433.CrossRefGoogle Scholar
  10. 10.
    Srilalitha, V., Raghavendra Guru Prasad, A., Raman Kumar, K., Seshagiri, V., and Ravindranath, L.K., Bul. Stiint. Univ. “Politeh.” Timisoara, Ser. Chim. Ing. Mediului, 2010, vol. 55, p. 107.Google Scholar
  11. 11.
    Maharramov, A.M., Zalov, A.Z., Verdizadeh, N.A., and Hajieva, A.B., Int. J. Chem. Stud., 2015, vol. 3, p. 88.Google Scholar
  12. 12.
    Bhadwaj, S. and Kakkar, L.R., Der. Chim. Sin., 2013, vol. 4, p. 32.Google Scholar
  13. 13.
    Zalov, A.Z., Verdizade, N.A., and Hadjieva, A.B., Pak. J. Anal. Environ. Chem., 2015, vol. 16, p. 16.Google Scholar
  14. 14.
    Rana, P.K., Lokhande, R.S., Pitale, S.M., Janwadkar, S.P., Navarkar, P.S., and Yadav, D.K., J. Pharm. Sci. Innovation, 2012, vol. 1, no. 5, p. 20.Google Scholar
  15. 15.
    Satheesh, K.P., Ravichandran, S., Suryanarayana Rao, V., Devanna, N., and Chandrasekhar, K.B., Int. J. Chem. Tech. Res., 2011, vol. 3, p. 1740.Google Scholar
  16. 16.
    Kodama, K., Methods of Quantitative Inorganic Analysis, New York: Interscience, 1963.Google Scholar
  17. 17.
    Algar, J. and Flynn, J.P., Proc. R. Ir. Acad., Sect. B, 1934, vol. 42, p. 1.Google Scholar
  18. 18.
    Oyamada, T., J. Chem. Soc. Jpn., 1934, vol. 55, p. 1256.Google Scholar
  19. 19.
    Prakash, O., Kumar, R., and Parkash, V., Eur. J. Med. Chem., 2008, vol. 43, p. 435.CrossRefGoogle Scholar
  20. 20.
    Kumar, A., Dass, R., and Sharma, R.G., Chem. Anal. (Warsaw), 2005, vol. 50, p. 625.Google Scholar
  21. 21.
    Ringbom, A., Z. anal. Chem., 1938, vol. 115, p. 332.CrossRefGoogle Scholar
  22. 22.
    Job, P., Ann. Chim., 1928, vol. 9, p. 113.Google Scholar
  23. 23.
    Vosburgh, W.C. and Cooper, G.R., J. Am. Chem. Soc., 1941, vol. 63, p. 437.CrossRefGoogle Scholar
  24. 24.
    Yoe, J.H. and Jones, A.L., Ind. Eng. Chem., Anal. Ed., 1944, vol. 16, p. 111.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Department of Applied Science, UIET, Kurukshetra UniversityKurukshetraIndia
  2. 2.Department of Chemistry, Maharishi Markandeshwar UniversityMullana, AmbalaIndia

Personalised recommendations