Journal of Analytical Chemistry

, Volume 73, Issue 9, pp 917–921 | Cite as

Determination of Non-Narcotic Analgetics by HPLC with Preconcentration on Triton X-100 Induced Phases

  • O. I. FedorchukEmail author
  • S. A. Kulichenko


The induced micellar-extraction preconcentration of mefenamic acid, naproxen, and indomethacin on Triton X-100 phases in the presence of salicylic and benzoic acids is studied. The optimal conditions for the preconcentration of analgetics are found: the pH of the solutions and the concentration of analytes. The maximum extraction of analgetics is observed at pH < pKa of the analyte and modifier. An increase in the recovery of analgetics with a decrease in their concentration in the initial solution is shown. A procedure for the determination of non-narcotic analgetics by HPLC with preliminary micellar-extraction concentration on Triton X-100 induced phases is developed. The procedure was used to analyze an urine sample with additives.


non-narcotic analgetics Triton X-100 induced phases preconcentration HPLC 



  1. 1.
    Pugajeva, I., Rusko, J., Perkons, I., Lundanes, E., and Bartkevics, V., J. Pharm. Biomed. Anal., 2017, vol. 133, p. 64.CrossRefPubMedGoogle Scholar
  2. 2.
    Hasan, N., Chaiharn, M., Toor, U.A., Mirani, Z.A., Sajjad, G., Sher, N., Aziz, M., and Siddiqui, F.A., Open Med. Chem. J., 2016, vol. 10, p. 33.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Talaat, W., Biomed. Chromatogr., 2017, vol. 31, no. 5, p. 1002.CrossRefGoogle Scholar
  4. 4.
    Ramos-Payan, M., Maspoch, S., and Llobera, A., Anal. Chim. Acta, 2016, vol. 946, p. 56.CrossRefPubMedGoogle Scholar
  5. 5.
    Savvin, S.B., Chernova, R.K., and Shtykov, S.N., Poverkhnostno-aktivnye veshchestva (Surfactants), Moscow: Nauka, 1991.Google Scholar
  6. 6.
    Leszek, M., Langmuir, 1990, vol. 6, no. 2, p. 347.CrossRefGoogle Scholar
  7. 7.
    Klaus, A., Tiddy, G.J., Rachel, R., Trinh, A.P., Maurer, E., Touraud, D., and Kunz, W., Langmuir, 2011, vol. 27, no. 8, p. 4403.CrossRefPubMedGoogle Scholar
  8. 8.
    Prasad, M., Moulik, S.P., Chisholm, D., and Palepu, R., J. Oleo Sci., 2003, vol. 52, no. 10, p. 523.CrossRefGoogle Scholar
  9. 9.
    Fedorchuk, O.I., and Kulichenko, S.A., Dokl. Akad. Nauk Ukr., 2010, no. 10, p. 124.Google Scholar
  10. 10.
    Fedorchuk, O.I. and Kulichenko, S.A., Ukr. Khim. Zh., 2013, vol. 79, no. 8, p. 111.Google Scholar
  11. 11.
    Derzhavna farmakopeya Ukraini (State Pharmacopoeia of Ukraine), Kharkiv, 2014, 2nd ed., vol. 2.Google Scholar
  12. 12.
    Mashkovskii, M.D., Lekarstvennye sredstva (Medicinal Products) 2 vols., Moscow: Meditsina, 1998.Google Scholar
  13. 13.
    Madej, K., TrAC, Trends Anal. Chem., 2009, vol. 28, no. 4, p. 436.CrossRefGoogle Scholar
  14. 14.
    García-Reyes, J.F., Ortega-Barrales, P., and Molina-Díaz, À., Anal. Sci., 2007, vol. 23, no. 4, p. 423.CrossRefPubMedGoogle Scholar
  15. 15.
    Ibrahim, H., Boyer, A., Bouajila, J., Couderc, F., and Nepveu, F., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2007, vol. 857, no. 1, p. 59.CrossRefGoogle Scholar
  16. 16.
    Caturla, M.C. and Cusido, E., J. Chromatogr. A, 1992, vol. 581, no. 1, p. 101.CrossRefGoogle Scholar
  17. 17.
    Cruz-Vera, M., Lucena, R., Cárdenas, S., and Valcárcel, M., J. Chromatogr. A, 2008, vol. 1202, no. 1, p. 1.CrossRefPubMedGoogle Scholar
  18. 18.
    Es’haghi, Z. and Esmaeili-Shahri, E., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2014, vol. 973, p. 142.CrossRefGoogle Scholar
  19. 19.
    Helmy, S.A. and El-Bedaiwy, H.M., Ther. Drug Monit., 2014, vol. 36, no. 1, p. 100.PubMedGoogle Scholar
  20. 20.
    Sultan, M., Stecher, G., Stoggl, W.M., Bakry, R., Zaborski, P., Huck, C.W., El Kousy, N.M., and Bonn, G.K., Curr. Med. Chem., 2005, vol. 12, no. 5, p. 573.PubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  1. 1.Chemistry Department, Taras Shevchenko Kiev National UniversityKievUkraine

Personalised recommendations