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Journal of Analytical Chemistry

, Volume 71, Issue 8, pp 755–758 | Cite as

Spectrophotometric determination of lactic acid

  • L. N. Borshchevskaya
  • T. L. Gordeeva
  • A. N. Kalinina
  • S. P. Sineokii
Articles

Abstract

An efficient and inexpensive spectrophotometric method has been developed for the determination of lactic acid in the individual state and in food and biological and cultural liquids. The method is based on the spectrophotometric determination of the colored product of the reaction of lactate ions with iron(III) chloride at 390 nm. The optimum conditions of the reaction have been found, and a calibration curve in the range from 0.3 to 10 g/L of lactic acid with the correlation coefficient 0.9999 has been constructed. The method does not require complex sample preparation.

Keywords

spectrophotometry lactic acid iron(III) chloride 

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • L. N. Borshchevskaya
    • 1
  • T. L. Gordeeva
    • 1
  • A. N. Kalinina
    • 1
  • S. P. Sineokii
    • 1
  1. 1.State Research Institute of Genetics and Selection of Industrial MicroorganismsMoscowRussia

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