Journal of Analytical Chemistry

, Volume 71, Issue 8, pp 755–758 | Cite as

Spectrophotometric determination of lactic acid

  • L. N. Borshchevskaya
  • T. L. Gordeeva
  • A. N. Kalinina
  • S. P. Sineokii


An efficient and inexpensive spectrophotometric method has been developed for the determination of lactic acid in the individual state and in food and biological and cultural liquids. The method is based on the spectrophotometric determination of the colored product of the reaction of lactate ions with iron(III) chloride at 390 nm. The optimum conditions of the reaction have been found, and a calibration curve in the range from 0.3 to 10 g/L of lactic acid with the correlation coefficient 0.9999 has been constructed. The method does not require complex sample preparation.


spectrophotometry lactic acid iron(III) chloride 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Abercade Consulting. http://abercade. ru/research/analysis/7626.html. Cited November 5, 2015.Google Scholar
  2. 2.
    Miura, Y., Ohno, H., and Koh, T., Bunseki Kagaku, 1994, vol. 43, no. 10, p. 745.CrossRefGoogle Scholar
  3. 3.
    Lebuhn, M. and Hartmann, A., J. Chromatogr. A, 1993, vol. 629, p. 255.CrossRefGoogle Scholar
  4. 4.
    Lesh, M.J., Wilkinson, E.L., Zolfaghari, M.R., and Schreiber, M.A., J. Liq. Chromatogr., 1993, vol. 16, no. 11, p. 2415.CrossRefGoogle Scholar
  5. 5.
    Ohara, H., Hiraga, T., Katasho, I., Inuta, T., and Yoshida, T., J. Ferment. Bioeng., 1993, vol. 75, p. 470.CrossRefGoogle Scholar
  6. 6.
    Frledemann, T.E., Catonlo, M., and Shatter, P.A., J. Biol. Chem., 1927, vol. 73, p. 335.Google Scholar
  7. 7.
    Barker, S.B. and William, H., J. Biol. Chem., 1941, vol. 138, p. 535.Google Scholar
  8. 8.
    Paradis,D. and Nadeau,D., J. Tissue Cult. Methods, vol. 7, no. 4, p. 175.Google Scholar
  9. 9.
    Lactate colorimetric/fluorometric assay kit, BioVision. Cited November 5, 2015.Google Scholar
  10. 10.
    Amplite colorimetric L-lactate assay kit, AAT Bioquest. Cited November 5, 2015.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • L. N. Borshchevskaya
    • 1
  • T. L. Gordeeva
    • 1
  • A. N. Kalinina
    • 1
  • S. P. Sineokii
    • 1
  1. 1.State Research Institute of Genetics and Selection of Industrial MicroorganismsMoscowRussia

Personalised recommendations