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Journal of Analytical Chemistry

, Volume 70, Issue 10, pp 1225–1228 | Cite as

Profiling of bacterial cellular fatty acids by pyrolytic derivatization to 3-pyridylcarbinol esters

  • Slawomir KurkiewiczEmail author
  • Adam KurkiewiczEmail author
Articles

Abstract

Pyrolytic derivatization to 3-pyridylcarbinol esters coupled with gas chromatography-mass spectrometry (GC-MS) was applied to determine cellular fatty acid (CFA) profiles of Escherichia coli, Aerobacter aerogenes, Pseudomonas fluorescens, Bacillus subtilis and Clostridium perfringens. The structures of bacterial CFAs were inferred from the interpretation of mass spectra with electron ionization of the corresponding 3-pyridylcarbinol esters. In the method used, the entire bacterial cells are pyrolyzed in the presence of a derivatizing reagent, and CFA isolation prior to the GC-MS analysis is not required. The method is argued to be a cheap, fast and accurate alternative to classical esterification to 3-pyridylcarbinol esters, while still providing more detailed results than methods based on esterification to methyl esters. Significat reduction in required minimum bacterial mass allows for fast identification of the microorganisms, in particular slow growing bacteria species.

Keywords

pyrolysis-GC-MS 3-pyridylcarbinol fatty acid esters fast microorganism identification bacterial taxonomy cellular fatty acid identification 

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References

  1. 1.
    Li, Y., Wu, S., Wang, L., Shi, Y.L., and Wang, X., J. Sci. Food. Agric., 2010, vol. 90, no. 8, p. 1380.CrossRefGoogle Scholar
  2. 2.
    Denich, T.J., Beaudette, L.A., Lee, H., and Trevors, J.T., J. Microbiol. Methods, 2003, vol. 52, no. 2, p. 149.CrossRefGoogle Scholar
  3. 3.
    Raetz, C.R., Reynolds, C.M., Trent, M.S., and Bishop, R.E., Annu. Rev. Biochem., 2007, vol. 76, p. 295.CrossRefGoogle Scholar
  4. 4.
    Zhang, Y.M. and Rock, C.O., Nat. Rev. Microbiol., 2008, vol. 6, no. 3, p. 222.CrossRefGoogle Scholar
  5. 5.
    Giotis, E.S., McDowell, D.A., Blair, I.S., and Wilkinson, B.J., Appl. Environ. Microbiol., 2007, vol. 73, no. 3, p. 997.CrossRefGoogle Scholar
  6. 6.
    Di Pasqua, R., Hoskins, N., Betts, G., and Mauriello, G., J. Agric. Food Chem., 2006, vol. 54, no. 7, p. 2745.CrossRefGoogle Scholar
  7. 7.
    Tindall, B.J., Rossello-Mora, R., Busse, H.J., Ludwig, W., and Kampfer, P., Int. J. Syst. Evol. Microbiol., 2010, vol. 60, no. 1, p. 249.CrossRefGoogle Scholar
  8. 8.
    Lechevalier, M.P., Crit. Rev. Microbiol., 1977, vol. 5, no. 2, p. 109.CrossRefGoogle Scholar
  9. 9.
    Welch, D.F., Clin. Microbiol. Rev., 1991, vol. 4, no. 4, p. 422.Google Scholar
  10. 10.
    Dzierzewicz, Z., Cwalina, B., Kurkiewicz, S., Chodurek, E., and Wilczok, T., Appl. Environ. Microbiol., 1996, vol. 62, no. 9, p. 3360.Google Scholar
  11. 11.
    Lay, J.O., Gidden, J., Liyanage, R., Emerson, B., Durham, B., Lipid Technol., 2, vol. 24, no. 1, p. 11.Google Scholar
  12. 12.
    Lu, Y. and Harrington, P.B., Anal. Bioanal. Chem., 2010, vol. 397, no. 7, p. 2959.CrossRefGoogle Scholar
  13. 13.
    Harvey, D.J., Biomed. Mass Spectrom., 1982, vol. 9, no. 1, p. 33.CrossRefGoogle Scholar
  14. 14.
    Harvey, D.J., Biomed. Mass Spectrom., 1984, vol. 11, no. 4, p. 187.CrossRefGoogle Scholar
  15. 15.
    Harvey, D.J., Biomed. Mass Spectrom., 1984, vol. 11, no. 7, p. 340.CrossRefGoogle Scholar
  16. 16.
    Harvey, D.J., Mol. Biotech., 1998, vol. 10, no. 3, p. 251.CrossRefGoogle Scholar
  17. 17.
    Chiavari, G., Fabbri, D., and Prati, S., Chromatographia, 2001, vol. 53, nos. 5–6, p. 311.CrossRefGoogle Scholar
  18. 18.
    Dworzanski, J.P., Berwald, L., and Meuzelaar, H.L.C., Appl. Environ. Microbiol., 1990, vol. 56, no. 6, p. 1717.Google Scholar
  19. 19.
    Dworzanski, J.P., Berwald, L., McClennen, W.H., and Meuzelaar, H.L.C., Appl. Environ. Microbiol., 1991, vol. 21, nos. 1–2, p. 221.Google Scholar
  20. 20.
    Kurkiewicz, S., Dzierzewicz, Z., Wilczok, T., and Dworzański, J.P., J. Am. Soc. Mass Spectrom., 2003, vol. 14, no. 1, p. 58.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  1. 1.Department of Instrumental Analysis, Faculty of PharmacyMedical University of SilesiaSosnowiecPoland
  2. 2.School of Computing ScienceUniversity of GlasgowGlasgowScotland

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