Journal of Analytical Chemistry

, Volume 69, Issue 13, pp 1215–1219 | Cite as

Ion mobility spectrometry coupled to laser-induced fluorescence for probing the electronic structure and conformation of gas-phase ions

  • V. E. Frankevich
  • K. V. Barylyuk
  • P. Martinez-Lozano Sinues
  • R. Zenobi


We report on an improved design of a differential ion mobility analyzer (DMA) coupled to laser-induced fluorescence (LIF) for the simultaneous retrieval of two-dimensional information on the electric mobility and fluorescence spectroscopy of gas-phase ions. This enhanced design includes an ion funnel inter-face at the input orifice of the DMA and a nozzle beam stage at the output of the DMA. These improvements allow the detection of fluorescence not only from pure dyes and their clusters, as was demonstrated recently, but also from fluorophore-tagged biomolecules. Complex mixtures of fluorescent compounds can be separated by the DMA and studied by LIF. This unique combination of instruments also provides a powerful platform for probing fluorescent proteins in the gas phase. The green fluorescent protein (GFP) was tested on a new setup. In contrast to high vacuum, where no GFP fluorescence was detected, the presence of a LIF signal at the output of the DMA could explain some specific fluorescent properties of GFP in the gas phase. Given that both conformation and fluorescence are key properties of biological molecules in the gas phase, we expect that our enhanced design will answer the question whether gas-phase proteins retain their liquid-phase native structure or not.


differential ion mobility analyzer laser-induced fluorescence green fluorescent protein electro-spray gas-phase ions soft ionization native proteins 


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  1. 1.
    Lakowicz, J.R., Principles of Fluorescence Spectroscopy, Berlin: Springer, 2006.CrossRefGoogle Scholar
  2. 2.
    Frankevich, V., Guan, X., Dashtiev, M., and Zenobi, R., Eur. J. Mass Spectrom., 2005, vol. 11, no. 5, p. 475.CrossRefGoogle Scholar
  3. 3.
    Chingin, K., Chen, H.W., Gamez, G., and Zenobi, R., J. Am. Soc. Mass Spectrom., 2009, vol. 20, no. 9, p. 1731.CrossRefGoogle Scholar
  4. 4.
    Chingin, K., Balabin, R.M., Barylyuk, K.V., Chen, H.W., Frankevich, V., and Zenobi, R., Phys. Chem. Chem. Phys., 2010, vol. 12, no. 37, p. 11710.CrossRefGoogle Scholar
  5. 5.
    Frankevich, V., Barylyuk, K., Chingin, K., Nieckarz, R., and Zenobi, R., Chem. Phys. Chem., 2013, vol. 14, no. 5, p. 929.Google Scholar
  6. 6.
    Zimmer, M., Chem. Rev., 2002, vol. 102, no. 3, p. 759.CrossRefGoogle Scholar
  7. 7.
    Fenn, J., Mann, M., Meng, C., Wong, S., and Whitehouse, C., Science, 1989, vol. 246, no. 4926, p. 64.CrossRefGoogle Scholar
  8. 8.
    Karas, M. and Krüger, R., Chem. Rev., 2003, vol. 103, no. 2, p. 427.CrossRefGoogle Scholar
  9. 9.
    Chingin, K., Frankevich, V., Balabin, R.M., Barylyuk, K.V., Chen, H.W., Wang, R., and Zenobi, R., Angew. Chem., 2010, vol. 49, no. 13, p. 2358.CrossRefGoogle Scholar
  10. 10.
    Frankevich, V., Martinez-Lozano, Sinues P., Barylyuk, K., and Zenobi, R., Anal. Chem., 2013, vol. 85, no. 1, p. 39.CrossRefGoogle Scholar
  11. 11.
    Fernandez de la Mora, J., The Differential Mobility Analyzer (DMA): Adding a True Mobility Dimension to a Preexisting API-MS, in Ion Mobility Spectrometry — Mass Spectrometry: Theory and Applications, Wilkins, C. and Trimpin, S., Eds., CRC Press, 2010.Google Scholar
  12. 12.
    Rauschenbach, S., Pauly, M., Küster, A., Kern, K., Reiss, J., Sesterhenn, J., and Sroka, M., European Patent Application EP 2631930A1, 2013.Google Scholar
  13. 13.
    Kelly, R.T., Tolmachev, A.V., Page, J.S., Tang, K., and Smith, R.D., Mass Spectrom. Rev., 2010, vol. 29, no. 2, p. 294.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • V. E. Frankevich
    • 1
  • K. V. Barylyuk
    • 1
  • P. Martinez-Lozano Sinues
    • 1
  • R. Zenobi
    • 1
  1. 1.Department of Chemistry and Applied BiosciencesETH ZurichZurichSwitzerland

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