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Journal of Analytical Chemistry

, Volume 69, Issue 2, pp 200–204 | Cite as

Generation of characteristic profiles of steroid hormones by reversed-phase HPLC

  • E. V. Obedkova
  • L. A. Kartsova
  • D. O. Kirsanov
  • L. I. Velikanova
  • A. V. Legin
Articles
  • 92 Downloads

Abstract

A method for the determination of steroid hormones by reversed-phase HPLC has been applied to the analysis of biological fluids from healthy individuals and patients with endocrine systems diseases. The obtained chromatographic profiles were processed by the method of principal component analysis and method of soft independent modeling of class analogy. The principle possibility of using the results of chemometric processing of the chromatographic profiles as an additional diagnostic criterion for the treatment of some endocrine pathologies has been shown.

Keywords

chromatographic profiles high-performance liquid chromatography steroid hormones chemometric processing method of principal component analysis 

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • E. V. Obedkova
    • 1
  • L. A. Kartsova
    • 1
  • D. O. Kirsanov
    • 1
  • L. I. Velikanova
    • 2
  • A. V. Legin
    • 1
  1. 1.Department of ChemistrySt. Petersburg State UniversitySt. PetersburgRussia
  2. 2.Mechnikov North-Western State Medical UniversitySt. PetersburgRussia

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