Journal of Analytical Chemistry

, Volume 67, Issue 2, pp 156–162 | Cite as

A carbositall electrode modified with a SiO2-hemoglobin-gold film as a promising biosensing element

  • T. S. Rozhanchuk
  • O. Yu. Tananaiko
  • E. A. Mazurenko
  • O. A. Egorov


The analytical prospects of a carbositall electrode modified with a film based on SiO2, hemoglobin (Hb), and gold nanoparticles (CSE SiO2-Hb-Au), prepared by deposition with an electrically generated catalyst, are reported. Hemoglobin on the surface of CSE SiO2-Hb-Au is shown to possess electrocatalytic activity toward dissolved oxygen, giving a rationale for the development of a rapid voltammetric procedure of O2 quantification with a detection limit of 0.1 mg/L. The inhibition activity of a number of nitrogen-containing organic substances to the catalytic current CSE SiO2-Hb-Au is studied. A voltammetric procedure is proposed for determination of the anti-flu drug rimantadine, 1-(1-adamantyl)ethylamine hydrochloride, with a detection limit of 0.4 mg/L. The procedure is applicable to the quantification of rimantadine in blood serum and saliva.


silicon oxide film carbositall electrode hemoglobin rimantadine voltammetry 


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  1. 1.
    Budnikov, H.C., Maistrenko, V.N., and Vyaselev, M.R., Osnovy sovremennogo elektrokhimicheskogo analiza (Fundamentals of Modern Electrochemical Analysis), Moscow: Mir, 2003.Google Scholar
  2. 2.
    Budnikov, H.C., Zh. Anal. Khim., 2000, vol. 55, no. 11, p. 1133 [J. Anal. Chem. (Engl. Transl.), vol. 55, no. 11, p. 1014].Google Scholar
  3. 3.
    Shaidarova, L.G. and Budnikov, H.C., Zh. Anal. Khim., 2008, vol. 63, no. 10, p. 1014 [J. Anal. Chem. (Engl. Transl.), vol. 63, no. 10, p. 922].Google Scholar
  4. 4.
    Nadzhafova, O.Yu., Zaitsev, V., Drozdova, M., Vaze, A., and Rusling, J.F., Electrochem. Commun., 2004, vol. 6, no. 2, p. 205.CrossRefGoogle Scholar
  5. 5.
    Nadzhafova, O., Etienne, M., and Walcarius, A., Electrochem. Commun., 2007, vol. 9, no. 5, p. 1189.CrossRefGoogle Scholar
  6. 6.
    Wu, Y., Shen, Q., and Hu, Sh., Anal. Chim. Acta, 2006, vol. 558, nos. 1–2, p. 179.CrossRefGoogle Scholar
  7. 7.
    Chen, L. and Lu, G., J. Electroanal. Chem., 2006, vol. 597, no. 2, p. 51.Google Scholar
  8. 8.
    Dai, Zh., Liu, S., Ju, H., and Chen, H., Biosens. Bioelectron., 2004, vol. 19, no. 8, p. 861.CrossRefGoogle Scholar
  9. 9.
    Ma, X., Sun, Z., Zheng, X., and Li, G., Zh. Anal. Khim., 2006, vol. 61, no. 7, p. 725 [J. Anal. Chem. (Engl. Transl.), vol. 61, no. 7, p. 669].Google Scholar
  10. 10.
    Roat-Malone, R.M., Bioorganic Chemistry: A Short Cours, New Jersey: Wiley, 2002.CrossRefGoogle Scholar
  11. 11.
    Otravleniya v detskom vozraste (Poisoning in Children), Markova, I.V. and Abezgauz, A.M., Eds., Leningrad: Meditsina, 1971.Google Scholar
  12. 12.
    Zhang, Y., Cheng, W., Li, Sh., and Li, N., Coll. Surf., A, 2006, vol. 286, nos. 1–3, p. 33.CrossRefGoogle Scholar
  13. 13.
    Cheng, W.X., Jin, G.Y., and Zhang, Y.Z., Sens. Actuators, B, 2006, vol. 114, no. 1, p. 40.CrossRefGoogle Scholar
  14. 14.
    Fukuda, E.K., Rodriguez, L.C., Choma, N., Keigher, N., De Grazia, F., and Garland, W.A., Biomed. Environ. Mass. Spectrom., 1987, vol. 14, no. 1, p. 549.CrossRefGoogle Scholar
  15. 15.
    Harold, D.A., Anonick, P.K., Kinter, M., and Hayden, F.G., Clin. Chem., 1988, vol. 34, no. 8, p. 1597.Google Scholar
  16. 16.
    Rubio, F.A., Choma, N., and Fukuda, E.K., J. Chromatogr., 1989, vol. 497, no. 1, p. 147.Google Scholar
  17. 17.
    Choma, N., Davis, P.P., Edom, R.W., and Fukuda, E.K., Biomed. Chromatogr., 1992, vol. 6, no. 1, p. 12.CrossRefGoogle Scholar
  18. 18.
    Higashi, Y., Uemori, I., and Fujii, Y., Biomed. Chromatogr., 2005, vol. 19, no. 9, p. 655.CrossRefGoogle Scholar
  19. 19.
    Higashi, Y. and Fujii, Y., J. Chromatogr. Sci., 2005, vol. 43, no. 4, p. 213.Google Scholar
  20. 20.
    Shuangjin, C., Fang, F., Han, L., and Ming, M., J. Pharm. Biomed. Anal., 2007, vol. 44, no. 4, p. 1100.CrossRefGoogle Scholar
  21. 21.
    Xu, M., Ju, W., Xia, X., Tan, H., Chen, M., Zhang, J., Xiong, N., Jiang, M., Chen, L., and Gong, L., J. Chromatogr., B, 2008, vol. 864, nos. 1–3, p. 123.CrossRefGoogle Scholar
  22. 22.
    Walcarius, A., Chem. Mater., 2001, vol. 13, no. 10, p. 3351.CrossRefGoogle Scholar
  23. 23.
    Tan, X.C., Tian, Y.X., Cai, P.X., and Zou, X.Y., Anal. Bioanal. Chem., 2005, vol. 381, no. 2, p. 500.CrossRefGoogle Scholar
  24. 24.
    Azevedo, A.M., Prazeres, D.M.F., Cabral, J.M.S., and Fonseca, L.P., Biosens. Bioelectron., 2005, vol. 21, no. 2, p. 235.CrossRefGoogle Scholar
  25. 25.
    Kafi, A.K.M., Lee, D.Y., Park, S.H., and Kwon, Y.S., Thin Solid Films, 2008, vol. 516, no. 9, p. 2816.CrossRefGoogle Scholar
  26. 26.
    Wang, J., Anal. Chim. Acta, 1999, vol. 399, nos. 1–2, p. 21.CrossRefGoogle Scholar
  27. 27.
    Gill, I., Chem. Mater., 2001, vol. 13, no. 10, p. 3404.CrossRefGoogle Scholar
  28. 28.
    Braun, S., Rappoport, S., Zuzman, R., Avnir, D., and Ottolenghi, M., Mater. Lett., 2007, vol. 61, nos. 14–15, p. 2843.CrossRefGoogle Scholar
  29. 29.
    Gupta, R. and Chaudhury, N.K., Biosens. Bioelectron., 2007, vol. 22, no. 11, p. 2387.CrossRefGoogle Scholar
  30. 30.
    Rozhanchuk, T., Tananaiko, O., Mazurenko, I., Etienne, M., Walcarius, A., and Zaitsev, V., J. Electroanal. Chem., 2009, vol. 625, no. 1, p. 33.CrossRefGoogle Scholar
  31. 31.
    Chah, S., Hammond, M.R., and Zare, R.N., Chem. Biol., 2005, vol. 12, no. 3, p. 323.CrossRefGoogle Scholar
  32. 32.
    Nabivanets’, B.I., Sukhan, V.V., and Kalabina, L.V., Analitichna khimiya prirodnogo seredovishcha (Environmental analytical Chemistry), Kiev: Libid’,1996.Google Scholar
  33. 33.
    Kolb, V.G. and Kamyshnikov, V.S., Praktikum po klinicheskoi khimii (Practical Works of Clinical Chemistry), Minsk: Belarus’, 1982.Google Scholar
  34. 34.
    Sbornik sanitarno-gigienicheskikh normativov i metodov kontrolya vrednykh veshchestv v ob”ektakh okruzhayushchei prirodnoi sredy (Book of Sanitary-Hygienic Standards and Methods for Monitoring Harmful Substances in Environmental Samples), Moscow: Iskusstvo, 1991.Google Scholar
  35. 35.
    Osnovy analiticheskoi khimii (Basics of Analytical Chemistry), 2 vols., Zolotov, Yu.A., Ed., Moscow: Vysshaya Shkola, 2004, vol. 1.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • T. S. Rozhanchuk
    • 1
  • O. Yu. Tananaiko
    • 1
  • E. A. Mazurenko
    • 1
  • O. A. Egorov
    • 2
  1. 1.Faculty of ChemistryTaras Shevchenko Kiev National UniversityKievUkraine
  2. 2.VERKh-MS LaboratorySpoluka Chemical CompanyKievUkraine

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