Journal of Analytical Chemistry

, Volume 66, Issue 6, pp 618–622

Study on the interaction of DNA with resveratrol by resonance light scattering technique and its analytical application

  • Haiyan Xiang
  • Yang Xie
  • Jianding Huang


The interaction between resveratrol and DNA has been studied by resonance light scattering (RLS) technique. In strongly acidic solution, resveratrol has a maximum peak at 368 nm and the RLS intensity is remarkably enhanced by trace amounts of DNA due to its interaction with resveratrol. Based on this, a novel assay for nucleic acids has been developed. The characteristics of RLS, fluorescence and UV-VIS absorption spectra, the influential factors and optimum conditions of the reaction have been studied. The enhanced RLS intensity at 368 nm is proportional to the concentration of DNA within the range of 0–1600 μg/L for calf thymus DNA. The determination limit (3σ) is 5.2 ng/mL. The study of foreign substance effect on the determination of DNA indicates that most of metal ions have little effect on the determination of DNA. Three synthetic samples of DNA were analysed with satisfactory results. The results show that the proposed method is very sensitive, convenient, rapid and reproducible.


DNA resveratrol resonance light scattering technique 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Savouret, J.F. and Quesne, M., Biomed. Pharmacother, 2002, vol. 56, p. 84.CrossRefGoogle Scholar
  2. 2.
    Soleas, G.J., Diamandis E.P., and Goldberg D.M., Adv. Exp. Med. Biol., 2001, vol. 492, p. 159.CrossRefGoogle Scholar
  3. 3.
    Joseph, A.B. and David, A.S., Nature, 2006, vol. 5, p. 493.CrossRefGoogle Scholar
  4. 4.
    Xiang, H.Y. and Li, W.G., Electroanalysis, 2009, vol. 21, p. 1207.CrossRefGoogle Scholar
  5. 5.
    Chen, Z.G., Zhang, T.Y., Han, Y.L., and Zhu, L., Spectrochim. Acta A, 2006, vol. 65, p. 919.CrossRefGoogle Scholar
  6. 6.
    Gao, D.J., Tian, Y., Liang, F.H., Bi, S.Y., Li, T.C., Chen, Y.H., Zhang, H.Q., and Yu, A.M., Spectrochim. Acta A, 2007, vol. 66, p. 52.CrossRefGoogle Scholar
  7. 7.
    Wang, J., Li, Y.F., Huang, C.Z., and Wu, T., Anal. Chim. Acta, 2008, vol. 626, p. 37.CrossRefGoogle Scholar
  8. 8.
    Chen, Z.G., Zhu, L., Song, T.H., Chen, J.H., and Guo, Z.M., Spectrochim. Acta A, 2009, vol. 72, p. 518.CrossRefGoogle Scholar
  9. 9.
    Chen, H.Q., Luo, F.B., Liu, Y., Liang, A.N., and Lin, B., Wang, L., Spectrochim. Acta A, 2009, vol. 71, p. 1701.CrossRefGoogle Scholar
  10. 10.
    Xiao, X.L., Wang, Y.S., Chen, Z.M., Li, Q.X., Liu, H., Li, G.R., Lu, C.Y., Xue, J.H., and Li, Y.Z., Spectrochim. Acta A, 2008, vol. 71, p. 398.CrossRefGoogle Scholar
  11. 11.
    Cai, C.Q. and Chen, X.M., Spectrochim. Acta A, 2008, vol. 69, p. 592.CrossRefGoogle Scholar
  12. 12.
    Huang, C.Z., Li, K.A., and Tong, S.Y., Anal. Chem., 1996, vol. 68, p. 2259.CrossRefGoogle Scholar
  13. 13.
    Hao, Y.M. and Shen, H.X., Anal. Chim. Acta, 2000, vol. 422, p. 159.CrossRefGoogle Scholar
  14. 14.
    Zhou, H.P., Wu, X., and Yang, J.H., Talanta, 2009, vol. 78, p. 809.CrossRefGoogle Scholar
  15. 15.
    Li, L., Xu, Z.S., Pan, Q., and Song, G.W., J. Fluorine Chem., 2009, vol. 130, p. 567.CrossRefGoogle Scholar
  16. 16.
    Cai, C.Q., Chen, X.M., and Gong, H., Spectrochim. Acta A, 2009, vol. 72, p. 46.CrossRefGoogle Scholar
  17. 17.
    Li, Z.P., Li, K.A., and Tong, S.Y., Talanta, 2000, vol. 51, p. 63.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • Haiyan Xiang
    • 1
  • Yang Xie
    • 1
  • Jianding Huang
    • 1
  1. 1.School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhouChina

Personalised recommendations