Journal of Analytical Chemistry

, Volume 66, Issue 3, pp 290–295 | Cite as

Identification of coumarins in traditional Chinese medicine by direct-injection electrospray ionisation tandem mass spectrometry and high-performance liquid chromatography-mass spectrometry

  • Mei-E Yue
  • Jie Xu
  • Qian-Qian Li
  • Wan-Guo Hou
Articles

Abstract

A simple and rapid method has been developed for the identification of coumarins in the crude extract of traditional Chinese medicine by direct-injection electrospray ionisation (ESI) and tandem mass spectrometry (MS/MS). In this study, osthole (OS) were evaluated directly in the extract of Angelica dahurica Benth, Radix Angelicae pubescentis, and Fructus cnidii using this method. Imperatorin (IM) and its isomers isoimperatorins (IO) were detected in the three traditional Chinese medicines by direct injection ESI/MS/MS and confirmed by HPLC-ESI/MS. Our experimental data reveal that ESI/MS/MS is a sensitive, rapid and convenient technique to identify some constituents in complex and mixed plant extracts.

Keywords

coumarins traditional Chinese vedicine high-performance liquid chromatography-mass spectrometry 

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • Mei-E Yue
    • 1
  • Jie Xu
    • 1
  • Qian-Qian Li
    • 1
  • Wan-Guo Hou
    • 1
  1. 1.State Key Laboratory Base of Ecochemical Engineering, Lab of Colloids and Interfaces, College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoP.R. China

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