Impact of Bioorganic Additives of Different Nature on Aggregation Behavior and on Cloud Point Temperatures of Nonionic Surfactants Tergitol NP-7 and Triton X-114 in Buffer Solutions


Aqueous biphasic systems containing non-ionic ethoxylated surfactants are perspective as media for some biochemical reactions. A comprehensive study of phase and aggregation behavior of these micellar systems, as well as consideration of the effects of biocomponents, their nature and concentration, pH and temperature are evident. In this work, the influence of salt medium (phosphate buffer), pH and temperature, as well as the presence of different bioorganic additives on cloud point and structural-thermodynamic properties (CMC and aggregation number) were studied for micellar solutions of non-ionic surfactants Triton X-114 and Tergitol NP-7 in water and buffers with pH 6.8, 4.3, 2.7. As additives were used the components of a biocatalytic hydrolysis reaction of penicillin G, namely penicillin G potassium salt, phenylacetic acid, and 6-aminopenicillanic acid. The different effects of the biocomponents on the cloud point and CMC value of Triton X-114 in the buffer with pH 4.3 are discussed. In addition, it was shown that the CMC value and the aggregation number of Triton X-114 decrease in buffer solutions (salting-out effect) and the CMC value increase at growing the temperature to 37°C (what is typical for ethoxylated non-ionic surfactants). The addition of phenylacetic acid (10 mM) leads to a reduction in the aggregation number of Triton X-114 at pH 4.3; other bio-additives have practically no effect. Thus, the studied additives have different effects on the structure and phase behavior of investigated micellar solutions, and this is an important aspect for choosing an extraction system to carry out a bioreaction.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.


  1. 1

    Wang, Z., Appl. Microbiol. Biotechnol., 2007, vol. 75, p. 1.

  2. 2

    Wang, Z., Appl. Microbiol. Biotechnol., 2011, vol. 89, p. 523.

  3. 3

    Wang, L., Wang, Z., Xu, J.-H., Bao, D., and Qi, H., Bio-process Biosyst. Eng., 2006, vol. 29, p. 157.

  4. 4

    Wang, Z., Guo, Y., Bao, D., and Qi, H., J. Chem. Technol. Biotechnol., 2006, vol. 81, p. 560.

  5. 5

    Ritter, E., Yordanova, D., Gerlach, T., Smirnova, I., and Jakobtorweihen, S., Fluid Phase Equilib., 2016, vol. 422, p. 43.

  6. 6

    Amid, M., Manap, M.Y., Hussin, M., and Mustafa, S., Molecules, 2015, vol. 20, p. 11 184.

  7. 7

    Lopes, A.M., Santos-Ebinuma, V.C., Pessoa Júnior, A., and Rangel-Yagui, C.O., Braz. J. Chem. Eng., 2014, vol. 31, p. 1057.

  8. 8

    Diender, M.B., Straathof, A.J.J., and Heijnen, J.J., Biocatal. Biotransform., 1998, vol. 16, p. 275.

  9. 9

    Rao, B.R., Indian J. Chem., 2002, vol. 41B, p. 1697.

  10. 10

    Rusanov, A.I., Mitselloobrazovanie v rastvorakh poverkhnostno-aktivnykh veshchestv (Micellization in Surfactant Solutions), St. Petersburg: Khimiya, 1992.

  11. 11

    Selivanova, N.M., Kuzovkova, M.A., and Galyametdinov, Yu.G., Vestn. Kazansk. Tekhnol. Univ., 2013, vol. 16, no. 4, p. 85.

  12. 12

    Wolszczak, M. and Miller, J., J. Photochem. Photobiol. A, 2002, vol. 147, p. 45.

  13. 13

    Hinze, W.L. and Pramauro, E., CRCCrit. Rev. Anal. Chem., 1993, vol. 24, p. 133.

  14. 14

    Ritter, E. and Smirnova, I., Chem. Ing. Tech., 2018, vol. 90, p. 348.

  15. 15

    Koneva, A.S., Ritter, E., Lezov, A.A., Klestova, A.O., Smirnova, N.A., Safonova, E.A., and Smirnova, I., Colloids Surf. A, 2018, vol. 538, p. 45.

Download references


The reported study was funded by RFBR and DFG according to the research project no. 16-53-12 029 NNIO_а.

Author information

Correspondence to E. A. Safonova.

Ethics declarations

The authors declare that there is no conflict of interest.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Anufrikov, Y.A., Kondrakhina, P.S., Koneva, A.S. et al. Impact of Bioorganic Additives of Different Nature on Aggregation Behavior and on Cloud Point Temperatures of Nonionic Surfactants Tergitol NP-7 and Triton X-114 in Buffer Solutions. Colloid J 81, 627–633 (2019).

Download citation