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Study of the efficacy of photofrin®-Mediated PDT on human hepatocellular carcinoma (HepG2) cell line

  • Laser Methods in Chemistry, Biology, and Medicine
  • Published:
Laser Physics

Abstract

The present study evaluates the effects of photodynamic therapy (PDT) with Photofrin® using human liver cancer cells (HepG2) as an experimental model. We optimized the different PDT parameters, e.g. (time of incubation, optimal dose of light and drug concentration), cytotoxicity, phototoxicity, and cellular viability of the HepG2 cells has also been investigated in this experimental work. The effect of light on the viability of cells without the photosensitizer was examined firstly, HepG2 cell line was irradiated with red light (a diode laser, λ = 635 nm). The toxicity of the photosensitizer in the absence of light in current cell line was investigated secondly, Photofrin® has been used as photosensitizing agent. Optimal dose of light along with suitable concentration of Photofrin® were traced into HepG2 cell line, by means of spectrophotometric measurement. Cells viability was determined by means of neutral red assay (NRA). Finally, it was observed that no toxic effects with the absence of light, and no significant photodamage effect on the cells without the presence of photosensitizer were found, when studied independently. Our results showed that light doses of 100 J/cm2 gives effective PDT outcome for HepG2 cell line at photosensitizer concentration of 100 μg/ml.

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References

  1. C. M. N. Yow, C. K. Wong, Z. Huang, and R. J. Ho, Liv. Internat. 27, 201 (2007).

    Article  Google Scholar 

  2. Y. Xiao, F. Q. Yang, S. P. Li, G. Hu, S. M. Lee, and Y. T. Wang, W. J. Gastroenter. 14, 4309 (2008).

    Article  Google Scholar 

  3. H. B. E. Serag and A. C. Mason, N. Engl. J. Med. 340, 745 (1999).

    Article  Google Scholar 

  4. M. Date, K. Fukuchi, Y. Namiki, A. Okumura, S. Morita, H. Takahashi, and K. Ohura, Liv. Internat. 24, 142 (2004).

    Article  Google Scholar 

  5. B. B. Knowles, C. C. Howe, and D. P. Aden, Science 290, 497 (1980).

    Article  ADS  Google Scholar 

  6. B. Wilson, M. Patterson, and L. Lilge, Lasers Med. Sci. 12, 182 (1997).

    Article  Google Scholar 

  7. M. C. DeRosa and R. J. Crutchley, Coord. Chem. Rev. 233, 351 (2002).

    Article  Google Scholar 

  8. R. Bonnett, J. Heterocycl. Chem. 39, 455 (2002).

    Article  Google Scholar 

  9. R. Bonnett and G. Martínez, Tetrahedron 57, 9513 (2001).

    Article  Google Scholar 

  10. A. P. Castano, T. N. Demidova, and M. R. Hamblin, Photodiagn. Photodyn. Therapy 1, 279 (2004).

    Article  Google Scholar 

  11. V. R. Belkin, L. Chen, L. Fiedor, Y. Salomon, and A. Scherz, in Photodynamic Tumor Therapy (Harwood Acad., Amsterdam, 1998), pp. 117–125.

    Google Scholar 

  12. A. F. Mironov, A. N. Nizhnik, and A. Y. Nockel, J. Photochem. Photobiol. B: Biol. 4, 297 (1990).

    Article  Google Scholar 

  13. D. Kessel, P. Thompson, B. Musselman, and C. K. Chang, Cancer Res. 47, 4642 (1987).

    Google Scholar 

  14. S. Erfurth, W. Bauman, and E. Gragoudas, Ophthalmol. 101, 89 (1994).

    Google Scholar 

  15. K. Furuse, M. Fukuoka, H. Kato, K. N. Kubota, and Y. Kusunoki, J. Clin. Oncol. 11, 1852 (1993).

    Google Scholar 

  16. H. T. Whelan, M. H. Schmidt, and A. D. Segura, J. Neurosurg. 79, 562 (1993).

    Article  Google Scholar 

  17. L. Y. Xue, S. M. Chiu, and N. L. Oleinick, Exp. Cell Res. 263, 145 (2001).

    Article  Google Scholar 

  18. T. J. Dougherty, C. J. Gomer, and B. W. Henderson, J. Nat. Cancer Inst. 90, 889 (1998).

    Article  Google Scholar 

  19. N. Ahmad and H. Mukhtar, Methods Enzymol. 319, 342 (2000).

    Article  Google Scholar 

  20. Q. Peng, J. Moan, and J. M. Nesland, Ultrastruct. Patholog. 20, 109 (1996).

    Article  Google Scholar 

  21. R. R. Allison, G. H. Downie, R. Cuenca, H. X. Hua, C. J. H. Childs, and C. H. Sibata, Photodiagn. Photodyn. Ther. 1, 27 (2004).

    Article  Google Scholar 

  22. T. Mang, R. Allison, and G. Hewson, Cancer 91, 1 (2001).

    Article  Google Scholar 

  23. W. R. Potter, T. S. Mang, and T. J. Dougherty, Photochem. Photobiol. 46, 97 (1987).

    Article  Google Scholar 

  24. K. Takahira, M. Sano, H. Arai, and H. Hanai, Lasers Med. Sci. 19, 89 (2004).

    Article  Google Scholar 

  25. I. Kinzler, E. Haseroth, C. Hauser, and A. Ruck, Photochem. Photobiol. Sci. 6, 1332 (2007).

    Article  Google Scholar 

  26. H. H. Evans, M. Horng, and M. Ricanati, Photochem. Photobiol. 66, 690 (1997).

    Article  Google Scholar 

  27. F. Iwasa, S. Sassa, and A. Kappas, J. Biochem. 262, 807 (1989).

    Google Scholar 

  28. V. V. Coinsman, M. T. Foultier, L. X. D. Brito, L. Morlet, A. Gouyette, and T. Patrice, J. Photochem. Photobiol. B: Biol. 30, 201 (1995).

    Article  Google Scholar 

  29. M. Otake, M. Nishiwaki, Y. Kobayashi, S. Baba, E. Kohno, T. Kawasaki, Y. Fujise, and H. Nakamura, Bras. J. Cancer 89, 730 (2003).

    Article  Google Scholar 

  30. W. Stummer, A. Novotny, H. Stepp, C. Goetz, K. Bise, and H. J. Reulen, J. Neurosurg. 93, 1003 (2000).

    Article  Google Scholar 

  31. P. J. Muller and B. C. Wilson, Seminars Surg. Oncol. 11, 346 (1995).

    Article  Google Scholar 

  32. E. Papovic, A. Kaye, and J. Hill, J. Clin. Laser Radiat. Surg. 14, 251 (1996).

    Google Scholar 

  33. J. C. Tsai, Y. Y. Hsiao, L. J. Teng, C. T. Chen, and M. C. Kao, Lasers Surg. Med. 24, 296 (1999).

    Article  Google Scholar 

  34. M. Atif, S. Firdous, A. Khurshid, L. Noreen, S. S. Z. Zaidi, and M. Ikram, Laser Phys. Lett. 6, 886 (2009).

    Article  Google Scholar 

  35. M. Atif, M. F. Alam, S. Firdous, S. S. Z. Zaidi, R. Suleman, and M. Ikram, Laser Phys. Lett. 7, 757 (2010).

    Article  ADS  Google Scholar 

  36. A. Khursid, M. Atif, S. Firdous, S. S. Z. Zaidi, R. Salman, and M. Ikram, Laser Phys. 20, 1673 (2010).

    Article  ADS  Google Scholar 

  37. M. F. Alam, S. Roohi, M. Atif, S. Firdous, N. Amir, and R. Zahoor, Radioch. Act. 98, 813 (2010).

    Article  Google Scholar 

  38. Y. Z. Juarez, A. J. P. Zapata, L. S. Rodriguez, E. R. Gallegos, and A. C. Orea, Phys. Scr., T 118, 136 (2005).

    Article  ADS  Google Scholar 

  39. R. Cubeddu, G. Canti, P. Taroni, and G. Valentini, J. Photochem. Photobiol. B 29, 171 (1997).

    Article  Google Scholar 

  40. J. Webber, Y. Luo, and R. Crilly, J. Photochem. Photobiol. B 735, 209 (1996).

    Article  Google Scholar 

  41. M. Kriegmeir, R. Baumgartner, R. Kneuchel, H. Stepp, F. Hofsteder, and A. Hofstetter, J. Urol. 155, 105 (1996).

    Article  Google Scholar 

  42. J. C. Lai, P. C. Lo, D. K. P. Ng, W. H. Ko, S. C. H. Leung, K. P. Fung, and W. P. Fong, Cancer Biol. Ther. 5, 413 (2006).

    Article  Google Scholar 

  43. S. L. Jacques, S. Furuzawa, and T. Rodriguez, SPIE Proc. 2972, 74 (1997).

    Article  ADS  Google Scholar 

  44. S. Sharma, A. Jajoo, and A. Dube, J. Photochem. Photobiol. B: Biology 88, 156 (2007).

    Article  Google Scholar 

  45. A. Khaled, A. K. Leh, K. Omar, and M. S. Jaafar, Adv. Nature Sci. 2, 47 (2009).

    Google Scholar 

  46. K. R. Weishaupt, C. J. Gomer, and T. J. Dougherty, Cancer Res. 36, 2326 (1976).

    Google Scholar 

  47. P. C. Lo, J. D. Huang, D. Y. Y. Cheng, E. Y. M. Chan, W. P. Fong, W. H. Ko, and D. K. P. Ng, Chem. Eur. J. 10, 4831 (2004).

    Article  Google Scholar 

  48. Q. Peng, T. Warloe, J. Moan, A. Godal, F. Apricena, K. E. Giercksky, and J. M. Nesland, Cancer Res. 61, 5824 (2001).

    Google Scholar 

  49. Q. Peng, J. Moan, G. Farrants, H. E. Danielsen, and C. Rimington, Cancer Lett. 53, 129 (1990).

    Article  Google Scholar 

  50. Q. Peng, J. M. Nesland, J. Moan, J. F. Evensen, M. Kongshaug, and C. Rimington, Int. J. Cancer 45, 972 (1990).

    Article  Google Scholar 

  51. M. Atif, M. R. Stringer, J. E. C. Sawyer, and S. B. Brown, Photodiagn. Photodyn. Ther. 1, 173 (2004).

    Article  Google Scholar 

  52. M. Atif, S. Firdous, and M. Nawaz, Lasers Med. Sci. 25, 545 (2010).

    Article  Google Scholar 

  53. L. Liu, A. W. N. Leung, X. S. Xia, D. Q. Bai, H. D. Lin, and C. S. Xu, Laser Phys. Lett 8, 150 (2011).

    Article  ADS  Google Scholar 

  54. Y. Liu, P. Chen, F. Zhang, L. Lin, G. Q. Tang, and G. G. Mu, Laser Phys. Lett. 6, 465 (2009).

    Article  ADS  Google Scholar 

  55. J. Schlothauer, S. Hackbarth, and B. Roder, Laser Phys. Lett. 6, 216 (2009).

    Article  ADS  Google Scholar 

  56. Y. Y. Tian, F. Kong, X. Tian, Q. Guo, and F. A. Cui, Laser Phys. Lett. 5, 764 (2008).

    Article  ADS  Google Scholar 

  57. Y. Y. Tian, D. D. Xu, X. Tian, F. A. Cui, H. Q. Yuan, and W. N. Leung, Laser Phys. Lett. 5, 746 (2008).

    Article  ADS  Google Scholar 

  58. P. F. C. Menezes, H. Imasato, J. Ferreira, V. S. Bagnato, C. H. Sibata, and J. R. Perussi, Laser Phys. Lett. 5, 227 (2008).

    Article  ADS  Google Scholar 

  59. J. Ferreira, P. F. C. Menezes, C. Kurachi, C. Sibata, R. R. Allison, and V. S. Bagnato, Laser Phys. Lett. 5, 156 (2008).

    Article  ADS  Google Scholar 

  60. C. Lochmann, T. Haupl, and J. Beuthan, Laser Phys. Lett. 5, 151 (2008).

    Article  ADS  Google Scholar 

  61. S. Pratavieira, P. L. A. Santos, P. F. C. Menezes, C. Kurachi, C. H. Sibata, M. T. Jarvi, B. C. Wilson, and V. S. Bagnato, Laser Phys. 19, 1263 (2009).

    Article  ADS  Google Scholar 

  62. H. Ullah, M. Atif, S. Firdous, M. S. Mehmood, M. Ikram, C. Kurachi, C. Grecco, G. Nicolodelli, and V. S. Bagnato, Laser Phys. Lett. 7, 889 (2010).

    Article  Google Scholar 

  63. P. Chen, C. P. Zhang, Q. San, C. Z. Wang, Z. F. Yang, X. B. Fu, and H. W. Qian, Laser Phys. Lett. 7, 899 (2010).

    Article  Google Scholar 

  64. J. Ferreira, P. F. C. Menezes, C. H. Sibata, R. R. Allison, S. Zucoloto, O. Castro e Silva, and V. S. Bagnato, Laser Phys. 19, 1932 (2009).

    Article  ADS  Google Scholar 

  65. P. F. C. Menezes, H. Imasato, V. S. Bagnato, Cl. H. Sibata, and J. R. Perussi, Laser Phys. 19, 1457 (2009).

    Article  ADS  Google Scholar 

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Authors and Affiliations

  1. Department of Physics and Astronomy and KSU Research Chair for Laser Diagnosis of Cancer, College of Science, King Saud University, Riyadh, Saudi Arabia

    M. Atif

  2. Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, 45650, Pakistan

    M. Atif & M. Fakhar-e-Alam

  3. National Institute of Health, Islamabad, Pakistan

    S. S. Z. Zaidi & R. Suleman

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  1. M. Atif
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  2. M. Fakhar-e-Alam
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Original Text © Astro, Ltd., 2011

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Atif, M., Fakhar-e-Alam, M., Zaidi, S.S.Z. et al. Study of the efficacy of photofrin®-Mediated PDT on human hepatocellular carcinoma (HepG2) cell line. Laser Phys. 21, 1135–1144 (2011). https://doi.org/10.1134/S1054660X11110028

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  • DOI: https://doi.org/10.1134/S1054660X11110028

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