Laser Physics

, Volume 20, Issue 5, pp 1114–1118

Stimulated Raman scattering microscopy for live-cell imaging with high contrast and high sensitivity

Nonlinear Optics and Spectroscopy

DOI: 10.1134/S1054660X10090318

Cite this article as:
Ozeki, Y. & Itoh, K. Laser Phys. (2010) 20: 1114. doi:10.1134/S1054660X10090318


We describe the principle and experiment of stimulated Raman scattering (SRS) microscopy, which has various advantages such as high-contrast and high sensitivity. To discuss how these advantages are realized in SRS microscopy, we introduce an intuitive picture of SRS, where the SRS process is viewed as homodyne detection of a nonlinear-optical signal by the excitation pulse.

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Department of Material and Life ScienceGraduate School of Osaka UniversitySuite, OsakaJapan

Personalised recommendations