Russian Journal of Electrochemistry

, Volume 55, Issue 10, pp 962–969 | Cite as

Protein-Based Nanobiosensor for Electrochemical Determination of Hydrogen Peroxide

  • Z. Tamleh
  • R. RafipourEmail author
  • S. Kashanian


We designed a hydrogen peroxide biosensor. The electrochemical detection of H2O2 was performed based on immobilization of cobalt nanoparticles–ferritin (CoNPs–Fer) onto multiwalled carbon nanotubes (MWCNTS) ensnared into chitosan (CS) matrices. Electrochemical techniques such as differential pulse voltammetry (DPV) and cyclic voltammetry (CV) was used to check the property of biosensor. Energy-dispersive X-ray spectroscopy (EDXS) and field emission scanning electron microscopy (FESEM) techniques showed the prosperous immobilization of CoNPs–Fer on the modified GC electrode surface. The hydrogen peroxide-designed biosensor displayed a linear range from 0.2 to 14 nM (R2 = 0.99), a detection limit of 1.29 nM (S/N = 3) and sensitivity of –0.1105 μA/nM.The apparent heterogeneous electron transfer rate constant (Ks) and the charge transfer coefficient (α) were gained 4.19 s–1 and 0.49, respectively. This biosensor can detect hydrogen peroxide with high sensitivity, selectivity, and low detection limit.


nanobiosensor cobalt nanoparticles–ferritin hydrogen peroxide determination 



The authors declare that they have no conflict of interest.


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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Department of Chemistry, Kermanshah Branch, Islamic Azad UniversityKermanshahIran
  2. 2.Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) and Nanoscience and Nanotechnology Research Center (NNRC), Razi UniversityKermanshahIran
  3. 3.Nano Drug Delivery Research Center, Kermanshah University of Medical SciencesKermanshahIran

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