Craigia yunnanensis (Tiliaceae) is an endangered relic tree in China. Its wild populations have been seriously damaged and fragmented due to severe human disturbance. In this study, we developed microsatellite markers in order to study the variation of the mating system of C. yunnanensis. We collected 19 067 214 unigenes sequences by shallow sequencing of the genome. A bioinformatics screening identified 15 235 unique and putative microsatellites, from which 590 novel microsatellite markers were developed. We designed 70 primer pairs and successfully amplified 14 of them in 30 individuals. The number of alleles per locus ranged from 1 to 12. The observed and expected heterozygosities ranged from 0.000 to 1.000 and 0.000 to 0.906, respectively. These microsatellite loci will enrich the genetic resources to develop functional studies and conservation strategies for this endangered relict species.
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The study was conducted at the Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences. This work was supported by the National Natural Science Foundation of China (Grants no. 31600439), National Key R&D Program of China (2017YFC0505200) and Key Laboratory Construction of Yunnan Science and Technology Talents and Platform Program (Grant no. 2018DG004).
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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Chen, Y.L., Yang, J. & Sun, W.B. Development of 14 Microsatellite Markers in the Endangered Relict Plant Craigia yunnanensis (Tiliaceae). Russ J Genet 56, 123–127 (2020). https://doi.org/10.1134/S1022795420010032
- endangered species
- transcriptome sequencing
- Craigia yunnanensis