Molecular Variant Estimation of SLC11A1 Related Microsatellites in Chinese Indigenous Goats
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The aim of this study was to investigate the polymorphism of the SLC11A1 gene in Chinese indigenous goats. A total of 215 individuals from nine goat breeds were genotyped using two pairs of known SLC11A1. Thirty-four alleles were found in these breeds across two microsatellite loci. Observed heterozygosity ranged from 0.2292 ± 0.0607 in CDB to 0.3958 ± 0.0706 in CDM. The mean number of alleles ranged from 4.50 ± 2.12 in CDB to 7.50 ± 2.12 in CDM. The coefficient of inbreeding (FIS) was lowest in CDB (0.267) and highest in DZ (0.459). All populations deviated from HWE in these two markers, except for JZ and CDM populations, which had one (EX-15). According to the FST results, only nine pairs were significantly different. Bayesian analysis using the program STRUCTURE, as well as Neighbor-joining tree analysis, both expressed 2 clusters, revealing genetic clustering patterns consistent with geographic location. In summary, the results of this study indicated that nine breeds in the Chuan-Yu area were divided into two groups according to geographical distribution. Our study may provide guidance for future research investigating marker-assisted selection and disease-resistant breeding using the SLC11A1 gene.
Keywords:SLA11A1 goat genetic diversity
This work was supported by the Characteristic Germplasm Resources Population Selection and Innovation on Mutton Sheeps and Goats (no. 2015BAD03B05), National Natural Science Foundation of China (no. 31172195), People’s Livelihood Special Innovation Projects of CQ CSTC (no. cstc2016shmszx80064), and the Innovation Team Building Program in Chongqing universities (CXTDG201602004), Fundamental Research Funds for the Central Universities (XDJK2017A003), Fundamental Research Funds for the Central Universities (XDJK2018B014).
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Conflict of interest. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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