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Russian Journal of Genetics

, Volume 55, Issue 3, pp 301–308 | Cite as

Genomic Profiling of the Response of Aspergillus oryzae to the Treatment with Bis(2-Pyridine-1-Oxide) Diselenide

  • S. A. Zalepkina
  • V. F. SmirnovEmail author
  • A. V. Borisov
  • Zh. V. Matsulevich
GENETICS OF MICROORGANISMS
  • 3 Downloads

Abstract

We implemented genome-wide expression profiling to identify the mechanisms of toxicity of an organoselenium compound bis(2-pyridine-1-oxide) diselenide to the fungus А. oryzae RIB40. We uncovered changes in the expression levels in 72 genes. In particular, we observed a downregulation in the levels of several copper ion transmembrane transporter genes. In turn, we found a significant upregulation in the genes encoding oxidoreductases. The latter results are supported by biochemical experiments that revealed an increase in oxidoreductase activity in response to bis(2-pyridine-1-oxide) diselenide treatment. The results of a large-scale microarray analysis of the А. oryzae RIB 40 were confirmed by real-time quantitative PCR.

Keywords:

gene expression fungi organoselenium compounds DNA microarray examination PCR oxidoreductases 

Notes

ACKNOWLEDGMENTS

We are grateful to Prof. K. Gomi and the staff of the Bioindustrial Genomics Department of Tohoku University for providing the opportunities to conduct experiments and for consultations regarding these studies.

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • S. A. Zalepkina
    • 1
  • V. F. Smirnov
    • 1
    Email author
  • A. V. Borisov
    • 2
  • Zh. V. Matsulevich
    • 2
  1. 1.Department of Biochemistry and Biotechnology, Lobachevsky State University of Nizhny NovgorodNizhny NovgorodRussia
  2. 2.Department of Industrial Safety, Ecology, and Chemistry, Nizhny Novgorod State Technical UniversityNizhny NovgorodRussia

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