Bioinformatic Analysis of the Sciatic Nerve Transcriptomes of Mice after 30-Day Spaceflight on Board the Bion-M1 Biosatellite
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Comparative bioinformatic analysis of sciatic nerve transcriptomes of C57BL/6J mice was carried out. Animals were divided into three groups: Flight, 30-day spaceflight; Recovery, 30-day spaceflight with subsequent 7-day readaptation; and Control. A significant pool of genes with an absolute difference in expression of more than 32 times compared to the control group was revealed in mice after the 30-day spaceflight (Flight and Recovery groups). Comparative analysis of the Flight and Recovery groups of murine transcriptomes did not reveal any significant differences in gene expression. In animals after spaceflight on board the biosatellite, using the KEGG database (Kyoto Encyclopedia of Genes and Genomes), we identified genes related to the state of metabolic and signaling pathways involved in actin cytoskeleton regulation, regulation of potential-dependent calcium, sodium, and potassium channels, and myelination of nerve fibers.
Keywords:Bion-M1 biosatellite murine sciatic nerve transcriptome hypogravitational motor syndrome
We thank O.V. Tyapkina and K.A. Petrov for assistance in experiments.
This study was supported by the Russian Foundation for Basic Research grant no. 17-04-00385, the fundamental research program of the Presidium of the Russian Academy of Sciences “Basic Research for the Development of Biomedical Technologies,” and the subsidy allocated within the framework of state support of the Kazan (Volga Region) Federal University in order to increase its competitiveness among the world’s leading scientific and educational centers.
COMPLIANCE WITH ETHICAL STANDARDS
Conflict of interests. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
- 1.Shenkman, B.S., From slow to fast: hypogravity-induced remodeling of muscle fiber myosin phenotype, Acta Nat., 20168, no. 4, pp. 47—59.Google Scholar
- 2.Grigoriev, A.I., Koslovskaya, I.B., and Shenkman, B.S., Role of support afferentation in organization of the tonic muscle system, Fiziol. Zh. im. I.M. Sechenova, 2004, vol. 90, no. 5, pp. 508—521.Google Scholar
- 11.Aziz, R., Verma, C.K., and Srivastava, N., Dimension reduction methods for microarray data: a review, AIMS Bioeng., 2017, no. 4, pp. 179—197. https://doi.org/10.3934/bioeng.2017.2.179
- 16.Piper, M. and Holt, C., RNA translation in axons, Annu. Rev. Cell Dev. Biol., 2004, vol. 20, no. 1, pp. 505—523. https://doi.org/10.1146/annurev.cellbio.20.010403.111746 CrossRefGoogle Scholar
- 17.Alvarez, J., Giuditta, A., and Koenig, E., Protein synthesis in axons and terminals: significance for maintenance, plasticity and regulation of phenotype: with a critique of slow transport theory, Prog. Neurobiol., 2000, vol. 62, no. 1, pp. 1—62. https://doi.org/10.1016/S0301-0082(99)00062-3 CrossRefGoogle Scholar
- 21.Kuznetsov, M.S., Rezvyakov, P.N., Lisyukov, A.N., et al., Transcriptomic profile of the mice sciatic nerve after 30-day space flight on the Bion-M1 biosatellite and subsequent 7-day readaptation on Earth, Aviakosm. Ekol. Med., 2017, vol. 51, no. 7, pp. 85—87. https://doi.org/10.21687/0233-528X-2017-51-7-85-87 Google Scholar