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Russian Journal of Genetics

, Volume 51, Issue 8, pp 737–744 | Cite as

The effect of introduction of the Heterologous gene encoding the N-acyl-homoserine lactonase (aiiA) on the properties of Burkholderia cenocepacia 370

  • V. A. Plyuta
  • V. A. Lipasova
  • O. A. Koksharova
  • M. A. Veselova
  • A. E. Kuznetsov
  • I. A. KhmelEmail author
Genetics of Microorganisms

Abstract

To study the role of Quorum Sensing (QS) regulation in the control of the cellular processes of Burkholderia cenocepacia 370, plasmid pME6863 was transferred into its cells. The plasmid contains a heterologous gene encoding AiiA N-acyl-homoserine lactonase, which degrades the signaling molecules of the QS system of N-acyl-homoserine lactones (AHL). An absence or reduction of AHL in the culture was revealed with the biosensors Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NT1/pZLR4, respectively. The presence of the aiiA gene, which was cloned from Bacillus sp. A24 in the cells of B. cenocepacia 370, resulted in a lack of hemolytic activity, reduced the extracellular proteolytic activity and decreased the cells’ ability to swarming migration on the surface of the agar medium. The introduction of the aiiA gene did not affect lipase activity, fatty acids synthesis, HCN synthesis, or biofilm formation. Hydrogen peroxide was shown to stimulate biofilm formation by B. cenocepacia 370 in concentrations that inhibited or weakly suppressed bacterial growth. The introduction of the aiiA gene into the cells did not eliminate this effect but it did reduce it.

Keywords

Quorum Sensing Luria Broth Quorum Sensing System Acyl Homoserine Lactone aiiA Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Inc. 2015

Authors and Affiliations

  • V. A. Plyuta
    • 1
    • 2
  • V. A. Lipasova
    • 1
  • O. A. Koksharova
    • 1
    • 3
  • M. A. Veselova
    • 1
  • A. E. Kuznetsov
    • 2
  • I. A. Khmel
    • 1
    Email author
  1. 1.Institute of Molecular GeneticsRussian Academy of SciencesMoscowRussia
  2. 2.Mendeleev University of Chemical Technology of RussiaMoscowRussia
  3. 3.Belozersky Institute of Physical and Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

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