Russian Journal of Genetics

, Volume 51, Issue 3, pp 278–288 | Cite as

Features of the B chromosome in Korean field mouse Apodemus peninsulae (Thomas, 1906) from Transbaikalia and the Far East identified by the FISH method

  • N. B. Rubtsov
  • I. V. Kartavtseva
  • G. V. Roslik
  • T. V. Karamysheva
  • M. V. Pavlenko
  • M. A. Iwasa
  • H. S. Koh
Animal Genetics


Korean field mouse (Apodemus peninsulae) is widely distributed throughout northeastern Asia, including the Russian Far East, northern China, the Korean Peninsula, Sakhalin, and Hokkaido. This mouse species is characterized by a high frequency of animals with B chromosomes differing in their number, morphology, and DNA composition in different geographical regions. For the first time a comparative analysis of DNA probes from B chromosomes with metaphase chromosomes of mice from Transbaikalia, the Far East (including the Russian Far East), Japan, and South Korea was conducted by in situ hybridization. B chromosomes in mice from the Russian Far East were shown to exhibit low variability in DNA content; however, the DNA composition of B chromosomes in species from Transbaikalia and Japan were highly variable. B chromosomes in A. peninsulae from the South Korean population demonstrate minor differences from those from the Russian Far East. We discuss the origin of B chromosomes in the studied region in comparison with previously obtained data for mice from Siberia and the Baikal region, as well as the dispersal routes of the Korean field mouse.


Pericentromeric Region Tick Bear Encephalitis rDNA Cluster Chro Mosomes Tick Bear Encephalitis Virus 
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  1. 1.
    Gromov, I.M. and Erbaeva, M.A., Mlekopitayushchie fauny Rossii i sopredel’nykh territorii: zaitseobraznye i gryzuny (The Mammals of Russia and Adjacent Territories (Lagomorphs and Rodents)), St. Petersburg: Zoologicheskiy Institut, 1995.Google Scholar
  2. 2.
    Kartavtseva, I.V. and Roslik, G.V., A complex B chromosome system in the Korean field mouse, Apodemus peninsulae, Cytogenet. Genome Res., 2004, vol. 106, nos. 2–4, pp. 271–278.CrossRefPubMedGoogle Scholar
  3. 3.
    Borisov, Yu.M., Afanas’ev, A.G., Lebedev, T.T., and Bochkarev, M.N., Multiplicity of B microchromosomes in a Siberian population of mice Apodemus peninsulae (2n = 48 + 4 − 30 B chromosomes), Russ. J. Genet., 2010, vol. 46, no. 6, pp. 705–711.CrossRefGoogle Scholar
  4. 4.
    Bekasova, T.S. and Vorontsov, N.N., Populational chromosome polymorphism in Asian wood mice Apodemus peninsulae (Rodentia, Muridae), Russ. J. Genet., 1975, vol. 11, no. 6, pp. 89–94.Google Scholar
  5. 5.
    Kartavtseva, I.V., Roslik, G.V., Pavlenko, M.V., et al., The B-chromosome system of the Korean field mouse Apodemus peninsulae in the Russian Far East, Chromosome Sci., 2000, vol. 4, pp. 21–29.Google Scholar
  6. 6.
    Rubtsov, N.B., Karamysheva, T.V., Kartavtseva, I.V., et al., B chromosomes: DNA, origin, and evolution, Biol. Membrany, 2005, vol. 22, no. 3, pp. 196–211.Google Scholar
  7. 7.
    Roslik, G.V. and Kartavtseva, I.V., Polymorphism and mosaicism of B chromosome number in Korean field mouse Apodemus peninsulae (Rodentia) in the Russian Far East, Cell Tissue Biol., 2010, vol. 4, no. 1, pp. 77–89.CrossRefGoogle Scholar
  8. 8.
    Koh, H.S., Systematic studies of Korean rodents: 2. A chromosome analysis in Korean field mice, Apodemus peninsulae peninsulae Thomas (Muridae, Rodentia), from Mungyong, with the comparison of morphometric characters of these Korean field mice to sympatric striped field mice, A. agrarius corea Thomas, Korean J. Syst. Zool., 1986, vol. 2, no. 1, pp. 1–10.Google Scholar
  9. 9.
    Abe, S., Han, S.H., Kojima, H., et al., Differential staining profiles of B-chromosomes in the East-Asiatic wood mouse Apodemus peninsulae, Chromosome Sci., 1997, vol. 1, no. 1, pp. 7–12.Google Scholar
  10. 10.
    Zima, J. and Macholán, M., B chromosomes in the wood mice (genus Apodemus), Acta Theriol., 1995, suppl. 3, pp. 75–86.Google Scholar
  11. 11.
    Borisov, Yu.M. and Malygin, V.M., The clinal variability of the B-chromosome system in the East Asian mouse Apodemus peninsulae from Buryatia and Mongolia, Tsitologiya, 1991, vol. 33, no. 1, pp. 106–111.Google Scholar
  12. 12.
    Borisov, Yu.M., Sheftel’, B.I., Safronova, L.D., and Aleksandrov, D.Yu., Stability of the population variants of the B-chromosome system in the East-Asian mouse Apodemus peninsulae from the Baikal region and Northern Mongolia, Russ. J. Genet., 2012, vol. 48, no. 10, pp. 1020–1028.CrossRefGoogle Scholar
  13. 13.
    Wang, J., Zhao, X., Qi, H., et al., Karyotypes and B chromosomes of Apodemus peninsulae (Rodentia, Mammalia), Acta Theriol. Sin., 2000, vol. 20, no. 4, pp. 289–296.Google Scholar
  14. 14.
    Hayata, I., Chromosomal polymorphism caused by supernumerary chromosomes in the field mouse, Apodemus giliacus, Chromosoma, 1973, vol. 42, pp. 403–414.CrossRefPubMedGoogle Scholar
  15. 15.
    Kartavtseva, I.V., Kariosistematika lesnykh i polevykh myshei (Rodentia, Muridae) (Karyosystematics of Wood and Field Mice (Rodentia, Muridae)), Vladivostok: Dal’nauka, 2002.Google Scholar
  16. 16.
    Karamysheva, T.V., Andreenkova, O.V., Bochkarev, M.N., et al., B chromosomes of Korean field mouse Apodemus peninsulae (Rodentia, Murinae) analyzed by microdis-section and FISH, Cytogenet. Genome Res., 2002, vol. 96, pp. 154–160.CrossRefPubMedGoogle Scholar
  17. 17.
    Rubtsov, N.B., Karamysheva, T.V., Andreenkova, O.V., et al., Comparative analysis of micro and macro B chromosomes in the Korean field mouse Apodemus peninsulae (Rodentia, Murinae) performed by chromosome microdissection and FISH, Cytogenet. Genome Res., 2004, vol. 106, pp. 289–294.CrossRefPubMedGoogle Scholar
  18. 18.
    Matsubara, K., Nishida-Umehara, C., Tsuchiya, K., et al., Karyotypic evolution of Apodemus (Muridae, Rodentia) inferred from comparative FISH analyses, Chromosome Res., 2004, vol. 12, pp. 383–395.CrossRefPubMedGoogle Scholar
  19. 19.
    Matsubara, K., Yamada, K., Umemoto, S., et al., Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia), Chromosome Res., 2008, vol. 16, pp. 1013–1026.CrossRefPubMedGoogle Scholar
  20. 20.
    Rubtsov, N.B., Borisov, Yu.M., Karamysheva, T.V., and Bochkarev, M.N., The mechanisms of formation and evolution of B chromosomes in Korean field mice Apodemus peninsulae (Mammalia, Rodentia), Russ. J. Genet., 2009, vol. 45, no. 4, pp. 389–396.CrossRefGoogle Scholar
  21. 21.
    Roslik, G.V. and Kartavtseva, I.V., B chromosome morphotypes of Apodemus peninsulae (Rodentia) from the Russian Far East, Tsitologiya, 2012, vol. 54, no. 1, pp. 66–77.Google Scholar
  22. 22.
    Hayasaka, D., Ivanov, L., Leonova, G.N., et al., Distribution and characterization of tick-borne encephalitis viruses from Siberia and Far-Eastern Asia, J. General Virol., 2001, vol. 82, pp. 1319–1328.Google Scholar
  23. 23.
    Serizawa, K., Suzuki, H., Iwasa, M., et al., A spatial aspect on mitochondrial DNA genealogy in Apodemus peninsulae from East Asia, Biochem. Genet., 2002, vol. 40, pp. 149–161.CrossRefPubMedGoogle Scholar
  24. 24.
    Sakka, H., Quéré, J.P., Kartavtseva, I., et al., Comparative phylogeography of four Apodemus species (Mammalia: Rodentia) in the Asian Far East: evidence of quaternary climatic changes in their genetic structure, Biol. J. Linn. Soc., 2010, vol. 100, no. 4, pp. 797–821.CrossRefGoogle Scholar
  25. 25.
    Vasilevskii, A.A., Kamennyi vek ostrova Sakhalin (The Stone Age of Sakhalin Island), Yuzhno-Sakhalinsk: Sakhalinsk Gos. Univ., 2008.Google Scholar
  26. 26.
    Mikishin, Yu.A., Gvozdeva, I.G., and Petrenko, T.I., Early Holocene of Sakhalin, in Aktual’nye problemy gumanitarnykh i estestvennykh nauk (Current Problems in Humanities and Natural Sciences), 2010, no. 12, pp. 432–437.Google Scholar
  27. 27.
    Kawamura, Y., Quaternary Rodent Faunas in the Japanese Islands, part 2, Memoirs of the Faculty Science Kyoto University, Series of Geology and Mineralogy, 1989, vol. 54, nos. 1–2.Google Scholar
  28. 28.
    Kirillova, I.V., Remains of vertebrates from the Tronnyi Grotto (Central Sakhalin), in Kraevedcheskii byulleten’: problemy istorii Sakhalina, Kuril i sopredel’nykh territorii (Local History Bulletin: History of Sakhalin, the Kuril Islands and Adjacent Territories), Yuzhno-Sakhalinsk: Sakhalinsk Gos. Univ., 2003, issue 2, pp. 128–137.Google Scholar
  29. 29.
    Burkovskii, O.A., The history of the rodent fauna formation (Mammalia, Rodentia) in Sakhalin, in Rastitel’nyi i zhivotnyi mir Sakhalina (Plant and Animal World of Sakhalin) (Proc. Int. Sakhalin Project), Vladivostok: Dal’nauka, 2004, part 1, pp. 238–248.Google Scholar
  30. 30.
    Zlobin, V.I., Belikov, S.I., Dzhioev, Yu.P., et al., The new concept of natural genetic variability of the tickborne encephalitis virus, Tikhookeansk. Med. Zh., 2001, no. 2, pp. 75–78.Google Scholar
  31. 31.
    Zlobin, V.I., Tick-borne encephalitis in Russian Federation: origin, epidemiology, and strategy of prophylaxis, Terra Med. Nova, 2010, no. 2, pp. 13–21.Google Scholar
  32. 32.
    Leonova, G.N., About nosological uniformity and evolution of tick-borne encephalitis, Tikhookeansk. Med. Zh., 2010, no. 3, pp. 19–22.Google Scholar
  33. 33.
    Golovljova, I., Katargina, O., Geller, J., et al., Unique signature amino acid substitution in Baltic tick-borne encephalitis virus (TBEV) strains within the Siberian TBEV subtype, Int. J. Med. Microbiol., 2008, vol. 298, pp. 108–120.CrossRefGoogle Scholar
  34. 34.
    Roslik, G.V., Kartavtseva, I.V., and Kosoy, M., Dotlike B-chromosomes in Korean field mice (Apodemus peninsulae, Rodentia) originated from a female artificially infected with tick-borne encephalitis virus, in Modern Achievements in Population, Evolutionary and Ecological Genetics (Proc. Intern. Symp.), Vladivostok, 1998, p. 15.Google Scholar
  35. 35.
    Yastrebov, V.K., Tick-borne encephalitis in Siberia: epidemiology, a combination of natural foci, Byull. Sib. Otd. Ross. Akad. Med. Nauk, 2007, no. 4, pp. 89–93.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2015

Authors and Affiliations

  • N. B. Rubtsov
    • 1
  • I. V. Kartavtseva
    • 2
  • G. V. Roslik
    • 2
  • T. V. Karamysheva
    • 1
  • M. V. Pavlenko
    • 2
  • M. A. Iwasa
    • 3
  • H. S. Koh
    • 4
  1. 1.Institute of Cytology and GeneticsSiberian Branch of the Russian Academy of SciencesNovosibirskRussia
  2. 2.Institute of Biology and Soil Science, Far Eastern BranchRussian Academy of SciencesVladivostokRussia
  3. 3.Nihon UniversityFujisawa, KanagawaJapan
  4. 4.Department of BiologyChungbuk UniversityCheonjuKorea

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