Russian Journal of Genetics

, Volume 49, Issue 9, pp 945–949 | Cite as

Microsatellite variation in white-spotted char Salvelinus leucomaenis from Sakhalin oblast

  • K. I. Afanas’ev
  • G. A. Rubtsova
  • E. G. Shaikhaev
  • L. A. Zhivotovskii
Animal Genetics


A panel of 12 polymorphic microsatellite markers was developed for the population genetic studies of white-spotted char Salvelinus leucomaensis. The four population samples examined consisted of 48 individuals each, and were collected in different geographical regions, including Sakhalin Island, Kunashir Island, and Iturup Island (two samples). The total number of different-sized alleles at different loci varied in the range of 2–31. In the population of white-spotted char subjected to strong anthropogenic pressure allelic diversity and expected heterozygosity indices were found to be lower than in wild populations of this species. The considerable genetic subdivision of different insular populations of white-spotted char observed was consistent with isolation by the distance model.


Chum Salmon MICROSATELLITE Variation Oncorhynchus Keta White Spotted Char Insular Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Esin, E.V. and Sorokin, Yu.V., Residential form of white-spotted char Salvelinus leucomaenis inhabiting the warm stream discharging into Semlyachikskii Firth (Kronotskii natural reserve, Kamchatka), Vopr. Ikhtiol., 2012, vol. 52, no. 2, pp. 207–214.Google Scholar
  2. 2.
    Savvaitova, K.A., Kuzishchin, K.V., Pichugin, M.Yu., et al., Systematics and biology of white-spotted char Salvelinus leucomaenis, Vopr. Ikhtiol., 2007, vol. 47, no. 1, pp. 58–71.Google Scholar
  3. 3.
    Yoshiyasu, K. and Humoto, Y., Starch-gel electrophoresis of hemoglobin of freshwater salmonid fishes in Southwest Japan: 1. Genus Salvelinus (char), Bull. Japn. Soc. Sci. Fish., 1972, vol. 38, pp. 779–788.CrossRefGoogle Scholar
  4. 4.
    Nakajima, M. and Fujio, Y., Genetic differentiation among local populations of Japanese char Salvelinus leucomaenis, Fish. Sci., 1995, vol. 61, pp. 11–15.Google Scholar
  5. 5.
    Yamamoto, S., Morita, K., Kitano, S., et al., Phylogeography of white-spotted char (Salvelinus leucomaenis) inferred from mitochondrial DNA sequences, Zool. Sci., 2004, vol. 21, pp. 229–240.PubMedCrossRefGoogle Scholar
  6. 6.
    Scribner, K.T., Crane, P.A., Spearman, W.J., and Seeb, L.W., DNA and allozyme markers provide concordant estimates of population differentiation: Analyses of US and Canadian populations of Yukon River fall-run chum salmon (Oncorhynchus keta), Can. J. Fish. Aquat. Sci., 1998, vol. 55, pp. 1748–1758.CrossRefGoogle Scholar
  7. 7.
    Small, M.P., Frye, A.E., Von Bargen, J.F., and Young, S.F., Genetic structure of chum salmon (Oncorhynchus keta) populations in the lower Columbia River: are chum salmon in Cascade tributaries remnant populations?, Conserv. Genet., 2006, vol. 7, pp. 65–78.CrossRefGoogle Scholar
  8. 8.
    Zhivotovskii, L.A., Afanas’ev, K.I., Rubtsova, G.I., et al., Creation of the DNA data base for solving reproduction, identification, and certification problems in pacific salmon population: an example of chum salmon of the Iturup Island, Vopr. Rybolov., 2008, vol. 9, no. 1(33), pp. 96–109.Google Scholar
  9. 9.
    Yamaguchi, K., Nakajima, M., and Taniguchi, N., Development of microsatellite markers in the Japanese char Salvelinus leucomaenis and its application to closely related species, Fish Genet. Breed. Sci., 2008, vol. 38, pp. 123–130.Google Scholar
  10. 10.
    Estoup, A., Presa, P., Krieg, F., et al., (CT)n and (GT)n microsatellites: a new class of genetic markers for Salmo trutta L. (brown trout), Heredity, 1993, vol. 71, pp. 488–496.PubMedCrossRefGoogle Scholar
  11. 11.
    Crane, P.A., Lewis, C.J., Kretchmer, E.J., et al., Characterization and inheritance of seven microsatellite loci from Dolly Varden, Salvelinus malma, and cross-species amplification in Arctic char, S. alpinus, Conserv. Genet., 2004, vol. 5, pp. 737–741.CrossRefGoogle Scholar
  12. 12.
    O’Reilly, P., Hamilton, L.C., McConnell, N., and Wright, J.M., Rapid detection of genetic variation in Atlantic salmon (Salmo salar) by PCR multiplexing of dinucleotide and tetranucleotide microsatellites, Can. J. Fish. Aquat. Sci., 1996, vol. 53, pp. 2292–2298.Google Scholar
  13. 13.
    Sanchez, J.A., Clabby, C., Ramos, D., et al., Protein and microsatellite single locus variability in Salmo salar L. (Atlantic salmon), Heredity, 1996, vol. 77, pp. 423–432.PubMedCrossRefGoogle Scholar
  14. 14.
    Olsen, J.B., Wilson, S.L., Kretschemer, E.J., et al., Characterization of 14 tetranucleotide microsatellite loci derived from sockeye salmon, Mol. Ecol., 2000, vol. 9, no. 12, pp. 2185–2187.PubMedCrossRefGoogle Scholar
  15. 15.
    Rollins, M.F., Vu, N.V., Spies, I.B., and Kalinowski, S.T., Twelve microsatellite loci for lake trout (Salvelinus namaycush), Mol. Ecol. Resour., 2009, vol. 9, pp. 871–873.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • K. I. Afanas’ev
    • 1
  • G. A. Rubtsova
    • 1
  • E. G. Shaikhaev
    • 1
  • L. A. Zhivotovskii
    • 1
  1. 1.Vavilov Institute of General GeneticsRussian Academy of SciencesMoscowRussia

Personalised recommendations