Russian Journal of Genetics

, Volume 48, Issue 8, pp 831–837 | Cite as

A panel of microsatellite loci for population studies of Sakhalin taimen Parahucho perryi (Brevoort)

  • M. V. Shitova
  • A. A. Yurchenko
  • E. G. Shaykhaev
  • L. A. Zhivotovsky
Animal Genetics


Using DNA samples of Sakhalin taimen and a set of microsatellite loci, earlier reported for other salmonid fishes (Salmonidae), successful cross-species amplification was performed. A total of 56 Sakhalin taimen (Parahucho perryi) samples from the Daga, Nabil, Poronai, and Agnevo rivers (Sakhalin Island) were examined at 36 microsatellite loci, most of which were described for other species and first tested in taimen. Among the 21 loci first tested in taimen, two loci produced no amplification products. The remaining 19 loci were successfully amplified (for some loci, new primers were generated). Thirteen of these loci were monomorphic, while six loci were polymorphic and used in further population genetic analysis. In addition, with the purpose of modification of the allele sizes and optimization of the research technique, new primers for the already known 12 loci of Sakhalin taimen were designed. Three more loci were included in analysis without changes. As a result, a joint panel consisting of 21 polymorphic microsatellite markers was suggested for analysis of Sakhalin taimen. This panel was tested with four population samples from the rivers of Sakhalin Island.The results showed that this panel of markers could be used in detailed population studies for evaluation of the level of genetic differentiation, inbreeding, and migrations in Sakhalin taimen, an endangered species with a fragmented range. Using this approach in further studies will make it possible to isolate basic populations, which is necessary for conservation of this rare species.


Microsatellite Locus Oncorhynchus Kisutch Tetranucleotide Repeat Genetic Diversity Index Amur Tiger 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Krasnaya kniga Rossiiskoi Federatsii (zhivotnye) (Red Book of the Russian Federation (Animals)), Moscow: Astrel’, 2001.Google Scholar
  2. 2.
    Zolotukhin, S.F., Semenchenko, A.Yu., and Belyaev, V.A., Taimeni i lenki Dal’nego Vostoka Rossii (Taimens and Lenoks of the Russian Far East), Khabarovsk: Priamurskoe geograficheskoe obshchestvo, 2000.Google Scholar
  3. 3.
    Bos, D.H. and Sites, Jr. J.W., Phylogeography and Conservation Genetics of the Columbia Spotted Frog (Rana luteiventris Amphibia, Ranidae), Mol. Ecol., 2001, vol. 10, pp. 1499–1513.PubMedCrossRefGoogle Scholar
  4. 4.
    Balakrishnan, C.N., Monfort, S.L., Gaur, A., et al., Phylogeography and Conservation Genetics of Eld’s Deer (Cervus eldi), Mol. Ecol., 2003, vol. 12, pp. 1–10.PubMedCrossRefGoogle Scholar
  5. 5.
    Alpers, D.L., Van Vuuren, B.J., Arctander, P., and Robinson, T.J., Population Genetics of the Roan Antelope (Hippotragus equinus) with Suggestion for Conservation, Mol. Ecol., 2004, vol. 13, pp. 1771–1784.PubMedCrossRefGoogle Scholar
  6. 6.
    Henry, P., Miquelle, D., Sugimoto, T., et al., In situ Population Structure and ex situ Representation of the Endangered Amur Tiger, Mol. Ecol., 2009, vol. 18, pp. 3173–3184.PubMedCrossRefGoogle Scholar
  7. 7.
    Hatakeyama, M., Watanabe, T., Ikeda, M., et al., Isolation and Characterization of Microsatellite DNA Loci for Endangered Fish, Japanese Huchen (Hucho perryi), Mol. Ecol. Notes, 2005, vol. 5, pp. 893–895.CrossRefGoogle Scholar
  8. 8.
    Kopun, Th., Winkler, K.A., and Weiss, S., Eight New Polymorphic Microsatellite DNA Markers for Sakhalin Taimen Parahucho perryi, Conserv. Genet., 2009, vol. 10, pp. 1089–1091.CrossRefGoogle Scholar
  9. 9.
    Crane, P.A., Lewis, C.J., Kretschmer, E.J., et al., Characterization and Inheritance of Seven Microsatellite Loci from Dolly Varden, Salvelinus malma, and Cross-Species Amplification in Arctic Char, S. alpinus, Conserv. Genet., 2004, vol. 5, pp. 737–741.CrossRefGoogle Scholar
  10. 10.
    Jackson, T.R., Ferguson, M.M., Danzmann, R.G., et al., Identification of Two QTL-Influencing Upper Temperature Tolerance in Three Rainbow Trout (Oncorhynchus mykiss) Half-Sib Families, Heredity, 1998, vol. 80, pp. 143–151.CrossRefGoogle Scholar
  11. 11.
    Rexroad, C.E.III, Coleman, R.L., Gustafson, A.L., et al., Development of Rainbow Trout Microsatellite Markers from Repeat Enriched Libraries, Mar. Biotechnol., 2002, vol. 3, pp. 12–16.CrossRefGoogle Scholar
  12. 12.
    Nelson, R.J. and Beacham, T.D., Isolation and Cross Species Amplification of Microsatellite Loci Useful for Study of Pacific Salmon, Anim. Genet., 1999, vol. 30, pp. 228–229.PubMedCrossRefGoogle Scholar
  13. 13.
    Smith, C.T., Koop, B.F., and Nelson, R.J., Isolation and Characterization of Coho Salmon (Oncorhynchus kisutch) Microsatellites and Their Use in Other Salmonids, Mol. Ecol., 1998, vol. 7, pp. 1614–1616.PubMedGoogle Scholar
  14. 14.
    Froufe, E.K., Sefc, M., Alexandrino, P., and Weiss, S., Isolation and Characterization of Brachymystax lenok Microsatellite Loci and Cross-Species Amplification in Hucho spp. and Parahucho perryi, Mol. Ecol. Notes, 2004, vol. 4, pp. 150–152.CrossRefGoogle Scholar
  15. 15.
    Williamson, K.S., Cordes, J.F., and May, B., Characterization of Microsatellite Loci in Chinook Salmon (Oncorhynchus tshawytscha) and Cross-Species Amplification in Other Salmonids, Mol. Ecol. Notes, 2002, vol. 2, pp. 17–19.CrossRefGoogle Scholar
  16. 16.
    Small, M.P., Beacham, T.D., Withler, R.E., and Nelson, R.J., Discriminating Coho Salmon (Oncorhynchus kisutch) Populations within Fraser River, British Columbia, Using Microsatellite DNA Markers, Mol. Ecol., 1998, vol. 7, pp. 141–155.CrossRefGoogle Scholar
  17. 17.
    Olsen, J.B., Wilson, S.L., Kretschemer, E.J., et al., Characterization of 14 Tetranucleotide Microsatellite Loci Derived from Sockeye Salmon, Mol. Ecol., 2000, vol. 9, pp. 2185–2187.PubMedCrossRefGoogle Scholar
  18. 18.
    Sanchez, J.A., Clabby, C., Ramos, D., et al., Protein and Microsatellite Single Locus Variability in Salmo salar L. (Atlantic Salmon), Heredity, 1996, vol. 77, pp. 423–432.PubMedCrossRefGoogle Scholar
  19. 19.
    Olsen, J.B. and Seeb, J.E., Genetic Interpretation of Broad-Scale Microsatellite Polymorphism in Odd-Year Pink Salmon, Trans. Am. Fish. Soc., 1998, vol. 127, pp. 535–550.CrossRefGoogle Scholar
  20. 20.
    Buchholz, W.G., Miller, S.J., and Spearman, W.J., Isolation and Characterization of Chum Salmon Microsatellite Loci and Use across Species, Anim. Genet., 2001, vol. 2, pp. 162–165.CrossRefGoogle Scholar
  21. 21.
    Slettan, A., Olsaker, I., and Lie, O., Segregation Studies and Linkage Analysis of Atlantic Salmon Microsatellites Using Haploid Genetics, Heredity, 1997, vol. 78, pp. 620–627.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • M. V. Shitova
    • 1
  • A. A. Yurchenko
    • 1
  • E. G. Shaykhaev
    • 1
    • 2
  • L. A. Zhivotovsky
    • 1
  1. 1.Vavilov Institute of General GeneticsRussian Academy of SciencesMoscowRussia
  2. 2.OOO “Isogene Laboratory,”MoscowRussia

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