Russian Journal of Genetics

, 47:1096 | Cite as

Comparative FISH analysis of C-positive regions of chromosomes of wood mice (Rodentia, Muridae, Sylvaemus)

  • N. B. Rubtsov
  • T. V. Karamysheva
  • A. S. Bogdanov
  • T. V. Likhoshvay
  • I. V. Kartavtseva
Animal Genetics


The homology of DNA of C-positive centromeric regions of chromosomes in wood mice of the genus Sylvaemus (S. uralensis, S. fulvipectus, S. sylvaticus, S. flavicollis, and S. ponticus) was estimated for the first time. DNA probes were generated by microdissection from the centromeric regions of individual autosomes of each species, and their fluorescence in situ hybridization (FISH) with metaphase chromosomes of representatives of all studied wood mouse species was carried out. Unlike in the chromosomal forms and races of S. uralensis, changes in the DNA composition of the chromosomal centromeric regions in the wood mouse species of the genus Sylvaemus (including closely related S. flavicollis and S. ponticus) are both quantitative and qualitative. The patterns of FISH signals after in situ hybridization of the microdissection DNA probes with chromosomes of the species involved in the study demonstrate significant differences between C-positive regions of wood mouse chromosomes in the copy number and the level of homology of repetitive sequences as well as in the localization of homologous repetitive sequences. It was shown that C-positive regions of wood mouse chromosomes can contain both homologous and distinct sets of repetitive sequences. Regions enriched with homologous repeats were detected either directly in C-positive regions of individual chromosomes or only on the short arms of acrocentrics, or at the boundary of C-positive and C-negative regions.


Centromeric Region Wood Mouse Pygmy Wood Mouse Large Autosome Small Autosome 
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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • N. B. Rubtsov
    • 1
    • 2
  • T. V. Karamysheva
    • 1
  • A. S. Bogdanov
    • 3
  • T. V. Likhoshvay
    • 2
  • I. V. Kartavtseva
    • 4
  1. 1.Institute of Cytology and Genetics, Siberian DivisionRussian Academy of SciencesNovosibirskRussia
  2. 2.Department of Cytology and GeneticsNovosibirsk State UniversityNovosibirskRussia
  3. 3.Kol’tsov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia
  4. 4.Institute of Biology and Soil Science, Far Eastern DivisionRussian Academy of SciencesVladivostokRussia

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