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Russian Journal of Genetics

, Volume 47, Issue 4, pp 404–408 | Cite as

Duration of the cell cycle phases in mutants for the tumor suppressor Merlin in Drosophila melanogaster

  • T. D. Dubatolova
  • N. V. Dorogova
  • L. V. Omel’yanchuk
  • L. -Sh. Chang
General Genetics

Abstract

The cell cycle duration was estimated in Drosophila melanogaster mutants for the tumor suppressor Merlin with the use of different approaches. Experiments on induction of mosaic clones in tissues of the larval wing imaginal disc showed that the cell cycle in mutant discs is shorter than that in control. Flow fluorescence cytometry revealed no differences between mutant and normal animals in the relative duration of the cell cycle phases, which suggests proportional shortening of the cell cycle phases. The study with pulselabeled mitoses confirmed these results and showed that the length of the cell cycle is 7 h (S phase duration 3 h) in control individuals and 5 h (S phase duration 2 h) in Merlin gene mutants.

Keywords

Cell Cycle Phase Imaginal Disc Phase Duration Clone Size Wing Imaginal Disc 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • T. D. Dubatolova
    • 1
  • N. V. Dorogova
    • 2
  • L. V. Omel’yanchuk
    • 1
    • 3
  • L. -Sh. Chang
    • 4
  1. 1.Institute of Chemical Biology and Fundamental Medicine, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  2. 2.Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  3. 3.Department of Cytology and Genetics, Department of Biomedical PhysicsNovosibirsk State UniversityNovosibirskRussia
  4. 4.Center for Childhood Cancer, Children’s Research Institute, and Department of PediatricsOhio State University College of MedicineColumbusUSA

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