TL, the new bacteriophage of Pseudomonas aeruginosa and its application for the search of halo-producing bacteriophages
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The properties of new virulent bacteriophage TL of Pseudomonas aeruginosa belonging to the family Podoviridae (genome size of 46 kb) were investigated. This bacteriophage is capable of lysing the bacterial lawn in halo zones around negative colonies (NC) of other bacteriophages. TL forms large NC, that are hardly distinguishable on the lawn of P. aeruginisa PAO1. At the same time, on the lawns of some phage-resistant PAO1 mutants, as well as on those produced by a number of clinical isolates, TL forms more transparent NC. It is suggested that more effective growth of the bacteriophage TL NC is associated with the differences in outer lipopolysaccharide (LPS) layer of the cell walls of different bacterial strains, as well as of the bacteria inside and outside of the halos. This TL property was used to optimize selection of bacteriophages producing halos around NC on the lawn of P. aeruginosa PAO1. As a result, a group of bacteriophages differing in the patterns of interaction between their halos and TL bacteriophage, as well as in some characters was identified. Taking into consideration the importance of cell-surfaced structures of P. aeruginosa in manifestation of virulence and pathogenicity, possible utilization of specific phage enzymes, polysacchadide depolymerases, for more effective treatment of P. aeruginosa infections is discussed.
KeywordsPseudomonas Aeruginosa Bacterial Lawn Halo Zone Phage Resistant Mutant Tail Plate
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- 9.Miller, J.H., Experiments in Molecular Genetics, New York: Cold Spring Harbor Laboratories, 1972.Google Scholar
- 10.Adams, M.H., Bacteriophages, New York: Interscience, 1959.Google Scholar
- 11.Sambrook, J., Fritsch, D.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Lab., 1989, 2nd, ed.Google Scholar
- 13.Shaburova, O.V., Krylov, S.V., Veiko, V.P., et al., Search for Destruction Factors of Bacterial Biofilms: Comparison of Phage Properties in a Group of Pseudomonas putida Bacteriophages and Specificity of Their Halo-Formation Products, Russ. J. Genet., 2009, vol. 45, no. 2, pp. 161–170.CrossRefGoogle Scholar