Russian Journal of Genetics

, Volume 46, Issue 10, pp 1233–1235 | Cite as

Role of G(-43)A polymorphism in the promoter region of the Xist gene in non-random x-chromosome inactivation in intraspecific hybrid voles

  • K. E. Orischenko
  • E. A. Elisaphenko
  • S. M. Zakian


Interaction of transcription factor CTCF with the minimal promoter of Xist gene was investigated in intraspecific hybrids of common voles. CTCF was shown to bind with the minimal promoter region in vivo. However, the EMSA experiments resulted in the absence of interaction between the CTCF factor and its potential binding site. Probably, G(-43)A substitution influences binding efficacy of another transcription factor such as activator protein 2, AP2.


Inner Cell Mass Minimal Promoter Common Vole CTCF Binding Potential Binding Site 
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  1. 1.
    Brockdorff, N., The Role of Xist in X-Inactivation, Curr. Opin. Genet. Dev., 1998, vol. 8, no. 3, pp. 328–333.CrossRefPubMedGoogle Scholar
  2. 2.
    Orstavik, K.H., Skewed X Inactivation in Healthy Individuals and in Different Diseases, Acta Paediatr. Suppl., vol. 95, no. 451, pp. 24–29.Google Scholar
  3. 3.
    Pugacheva, E.M., Tiwari, V.K., Abdullaev, Z., et al., Familial Cases of Point Mutations in the XIST Promoter Reveal a Correlation between CTCF Binding and Pre-Emptive Choices of X Chromosome Inactivation, Hum. Mol. Genet., 2005, vol. 14, no. 7, pp. 953–965.CrossRefPubMedGoogle Scholar
  4. 4.
    Chadwick, L.H. and Willard, H.F., Genetic and Parent-of-Origin Influences on X Chromosome Choice in Xce Heterozygous Mice, Mamm. Genome, 2005, vol. 16, no. 9, pp. 691–699.CrossRefPubMedGoogle Scholar
  5. 5.
    Zakian, S.M., Kulbakina, N.A., Meyer, M.N., et al., Non-Random Inactivation of the X-Chromosome in Interspecific Hybrid Voles, Genet. Res., 1987, vol. 50, no. 1, pp. 23–27.CrossRefPubMedGoogle Scholar
  6. 6.
    Orishchenko, K.E., Elisafenko, E.A., Kel’, A.E., et al., Molecular Genetic Characteristic of the Gene Xist Regulatory Region in the Vole Microtus rossiaemeridionalis, Russ. J. Genet., 2009, vol. 45, no. 10, pp. 1182–1191.CrossRefGoogle Scholar
  7. 7.
    Wylie, A.A., Murphy, S.K., Orton, T.C., et al., Novel Imprinted DLK1/GTL2 Domain on Human Chromosome 14 Contains Motifs That Mimic Those Implicated in IGF2/H19 Regulation, Genome Res., 2000, vol. 10, no. 11, pp. 1711–1718.CrossRefPubMedGoogle Scholar
  8. 8.
    Renda, M., Baglivo, I., Burgess-Beusse, B., et al., Critical DNA Binding Interactions of the Insulator Protein CTCF: A Small Number of Zinc Fingers Mediate Strong Binding, and a Single Finger-DNA Interaction Controls Binding at Imprinted Loci, J. Biol. Chem., 2007, vol. 282, no. 46, pp. 33336–33345.CrossRefPubMedGoogle Scholar
  9. 9.
    Donohoe, M.E., Zhang, L.F., Xu, N., et al., Identification of a Ctcf Cofactor, Yy1, for the X Chromosome Binary Switch, Mol. Cell, 2007, vol. 25, no. 1, pp. 43–56.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • K. E. Orischenko
    • 1
  • E. A. Elisaphenko
    • 1
  • S. M. Zakian
    • 1
  1. 1.Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia

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