Russian Journal of Genetics

, Volume 46, Issue 10, pp 1173–1177 | Cite as

Chicken lampbrush chromosomes: Transcription of tandemly repetitive DNA sequences

  • A. V. Krasikova
  • E. V. Vasilevskaya
  • E. R. Gaginskaya
Article

Abstract

The transcribed part of the genome includes both protein-coding sequences and a variety of sequences with unknown functions. Amphibian and avian lampbrush chromosomes represent a convenient experimental system for studying cell functions and the regulation of transcription of protein-noncoding DNA. Taking lumpy loops formed on chicken (Gallus gallus domesticus) chromosome 2 at the lampbrush stage as an example, we applied an approach allowing RNA sources to be identified in the lateral loops of lampbrush chromosomes. This approach involves a bioinformatic analysis of data from the chicken genome sequencing project and a high-resolution mapping of transcripts on microsurgically isolated bivalents. As a result, a novel tandemly repetitive DNA sequence, LL2R (lumpy loop 2 repeat), of ∼440 bp in size was identified in the chicken genome, its transcripts taking part in the formation of lumpy loops with a massive RNP matrix on chromosome 2 in growing oocytes.

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • A. V. Krasikova
    • 1
  • E. V. Vasilevskaya
    • 1
  • E. R. Gaginskaya
    • 1
  1. 1.Department of Cytology and HistologySt. Petersburg State UniversitySt. PetersburgRussia

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