Russian Journal of Genetics

, Volume 46, Issue 10, pp 1173–1177 | Cite as

Chicken lampbrush chromosomes: Transcription of tandemly repetitive DNA sequences

  • A. V. Krasikova
  • E. V. Vasilevskaya
  • E. R. Gaginskaya


The transcribed part of the genome includes both protein-coding sequences and a variety of sequences with unknown functions. Amphibian and avian lampbrush chromosomes represent a convenient experimental system for studying cell functions and the regulation of transcription of protein-noncoding DNA. Taking lumpy loops formed on chicken (Gallus gallus domesticus) chromosome 2 at the lampbrush stage as an example, we applied an approach allowing RNA sources to be identified in the lateral loops of lampbrush chromosomes. This approach involves a bioinformatic analysis of data from the chicken genome sequencing project and a high-resolution mapping of transcripts on microsurgically isolated bivalents. As a result, a novel tandemly repetitive DNA sequence, LL2R (lumpy loop 2 repeat), of ∼440 bp in size was identified in the chicken genome, its transcripts taking part in the formation of lumpy loops with a massive RNP matrix on chromosome 2 in growing oocytes.


Japanese Quail Chicken Genome Lampbrush Chromosome Oocyte Nucleus Chicken Genome Sequencing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Mattick, J.S. and Makunin, I.V., Non-Coding RNA, Hum. Mol. Genet., 2006, vol. 15,spec. no. 1, pp. 17–29.CrossRefGoogle Scholar
  2. 2.
    Morey, C. and Avner, P., Employment Opportunities for Non-Coding RNAs, FEBS Lett., 2004, vol. 567, no. 1, pp. 27–34.CrossRefPubMedGoogle Scholar
  3. 3.
    Lavrov, S.A. and Kibanov, M.V., Non-Coding RNAs and the Chromatin Structure, Usp. Biol. Khim., 2007, vol. 47, pp. 53–88.Google Scholar
  4. 4.
    Makarova, Yu.A. and Kramerov, D.A., Non-Coding RNAs, Biochemistry (Moscow), 2007, vol. 72, no. 11, pp. 1427–1448.Google Scholar
  5. 5.
    Prasanth, K.V. and Spector, D.L., Eukaryotic Regulatory RNAs: An Answer to the ‘Genome Complexity’ Conundrum, Genes Dev., 2007, vol. 21, no. 1, pp. 11–42.CrossRefPubMedGoogle Scholar
  6. 6.
    Davidson, E.H., Gene Activity in Early Development, Orlando: Academic, 1986, 3rd ed.Google Scholar
  7. 7.
    Morgan, G.T., Working with Oocyte Nuclei: Cytological Preparations of Active Chromatin and Nuclear Bodies from Amphibian Germinal Vesicles, Methods Mol. Biol., 2008, no. 463, pp. 55–66.Google Scholar
  8. 8.
    Gaginskaya, E., Kulikova, T., and Krasikova, A., Avian Lampbrush Chromosomes: A Powerful Tool for Exploration of Genome Expression, Cytogenet. Genome Res., 2009, vol. 124, nos. 3–4, pp. 251–267.CrossRefPubMedGoogle Scholar
  9. 9.
    Callan, H.G., Lampbrush Chromosomes, London: Springer-Verlag, 1986.Google Scholar
  10. 10.
    Gall, J.G., Wu, Z., Murphy, C., and Gao, H., Structure in the Amphibian Germinal Vesicle, Exp. Cell Res., 2004, vol. 296, pp. 28–34.CrossRefPubMedGoogle Scholar
  11. 11.
    Macgregor, H.C. and Andrews, C., The Arrangement and Transcription of ‘Middle Repetitive’ DNA Sequences on Lampbrush Chromosomes of Triturus, Chromosoma, 1977, vol. 63, pp. 109–126.CrossRefGoogle Scholar
  12. 12.
    Varley, J.M., Macgregor, H.C., and Erba, H.P., Satellite DNA Is Transcribed on Lampbrush Chromosomes, Nature, 1980, vol. 283, pp. 686–688.CrossRefPubMedGoogle Scholar
  13. 13.
    Kropotova, E.V. and Gaginskaya, E.R., Lampbrush Chromosomes from the Japanese Quail Oocytes, Tsitologiya, 1984, vol. 26, pp. 1008–1015.Google Scholar
  14. 14.
    Hutchison, N., Lampbrush Chromosomes of the Chicken, J. Cell Biol., 1987, vol. 105, pp. 1493–1500.CrossRefPubMedGoogle Scholar
  15. 15.
    Morgan, G.T., Macgregor, H.C., and Colman, A., Multiple Ribosomal Gene Sites Revealed by in situ Hybridization of Xenopus rDNA to Triturus Lampbrush Chromosomes, Chromosoma, 1980, vol. 80, pp. 309–330.CrossRefPubMedGoogle Scholar
  16. 16.
    Gaginskaya, E.R. and Gruzova, M.N., Detection of the Amplified rDNA in Ovarial Cells of Some Insects and Birds by Hybridization in situ, Tsitologiia, 1975, vol. 17, pp. 1132–1137.Google Scholar
  17. 17.
    Galkina, S., Deryusheva, S., Fillon, V., et al., FISH on Avian Lampbrush Chromosomes Produces Higher Resolution Gene Mapping, Genetics, 2006, vol. 128, nos. 1–3, pp. 241–251.Google Scholar
  18. 18.
    Krasikova, A., Deryusheva, S., Galkina, S., et al., On the Positions of Centromeres in Chicken Lampbrush Chromosomes, Chromosome Res., 2006, vol. 14, no. 7, pp. 777–789.CrossRefPubMedGoogle Scholar
  19. 19.
    Krasikova, A., Daks, A., Zlotina, A., and Gaginskaya, E., Polymorphic Heterochromatic Segments in Japanese Quail Microchromosomes, Cytogenet. Genome Res., 2009, vol. 126.Google Scholar
  20. 20.
    Hori, T., Susuki, Y., Solovei, I., et al., Characterization of DNA Sequences Constituting the Terminal Heterochromatin of the Chicken Z Chromosome, Chromosome Res., 1996, vol. 4, pp. 411–426.CrossRefPubMedGoogle Scholar
  21. 21.
    Deryusheva, S., Krasikova, A., Kulikova, T., and Gaginskaya, E., Tandem 41-bp Repeats in Chicken and Japanese Quail Genomes: FISH Mapping and Transcription Analysis on Lampbrush Chromosomes, Chromosoma, 2007, vol. 116, no. 6, pp. 519–530.CrossRefPubMedGoogle Scholar
  22. 22.
    Chelysheva, L.A., Solovei, I.V., Rodionov, A.V., et al., The Lampbrush Chromosomes of the Chicken: The Cytological Maps of the Macrobivalents, Tsitologiia, 1990, vol. 32, pp. 303–316.PubMedGoogle Scholar
  23. 23.
    Crooijmans, R., Vrebalov, J., Dijkhof, R., et al., Two-Dimensional Screening of the Wageningen Chicken BAC Library, Mamm. Genome, 2000, vol. 11, pp. 360–363.CrossRefPubMedGoogle Scholar
  24. 24.
    ICGSC (International Chicken Genome Sequencing Consortium): Sequence and Comparative Analysis of the Chicken Genome Provide Unique Perspectives on Vertebrate Evolution, Nature, 2004, vol. 432, pp. 695–716.CrossRefGoogle Scholar
  25. 25.
    Burge, C. and Karlin, S., Prediction of Complete Gene Structures in Human Genomic DNA, J. Mol. Biol., 1997, vol. 268, pp. 78–94.CrossRefPubMedGoogle Scholar
  26. 26.
    Dreyfuss, G., Matunis, M.J., Piol-Roma, S., and Burd, C.G., hnRNP Proteins and the Biogenesis of mRNA, Annu. Rev. Biochem., 1993, vol. 62, pp. 289–321.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • A. V. Krasikova
    • 1
  • E. V. Vasilevskaya
    • 1
  • E. R. Gaginskaya
    • 1
  1. 1.Department of Cytology and HistologySt. Petersburg State UniversitySt. PetersburgRussia

Personalised recommendations