Russian Journal of Genetics

, Volume 46, Issue 1, pp 50–56 | Cite as

Inheritance of marker and target genes in seed and vegetative progenies of transgenic tobacco plants carrying the buckwheat serine protease inhibitor gene

Plant Genetics

Abstract

The buckwheat serine protease inhibitor (BWI-1) target gene (ISP) was expressed under the control of the constitutive 35S promoter of the cauliflower mosaic virus was expressed in transgenic tobacco plants and conferred antibacterial resistance. A stable and linked inheritance and expression of the marker nptII and target genes were observed in a random sample of independent transgenic tobacco plants after longterm propagation by nodal segments or multiple (for 1.5 years) regenerations under nonselective conditions; the transgene insert was preserved in the T1 seed progeny. Transgenic plants displayed numerous alterations in microsporogenesis. A loss of kanamycin (Km) resistance was accompanied by a loss of antibacterial activity in two lines. Segregation was observed for Km resistance in line C7 and for seedling size in line C22.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Feldmann, K.A., Marks, D.A., Christianson, M.L., and Quatrano, R.S.A., Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis, Science, 1989, vol. 243, pp. 1351–1354.CrossRefPubMedGoogle Scholar
  2. 2.
    Souer, E., Krol, A., Kloos, D., et al., Genetic Control of Branching Pattern and Floral Identity during Petunia Inflorescence Development, Development, 1998, vol. 125, pp. 733–742.PubMedGoogle Scholar
  3. 3.
    Deineko, E.V., Novoselya, T.V., Zagorskaya, A.A., et al., Expression Stability of Marker Gene nptII in Transgenic Plants Nicotiana tabacum, Fiziol. Rastenii, 2000, vol. 47, pp. 446–452.Google Scholar
  4. 4.
    Nakano, Y. and Steward, N., Sekine, M., et al., A Tobacco NtMET1 cDNA Encoding a DNA Methyltransferase: Molecular Characterization and Abnormal Phenotypes of Transgenic Tobacco Plants, Plant Cell Physiol., 2000, vol. 41, pp. 448–457.CrossRefPubMedGoogle Scholar
  5. 5.
    Schepherd, L.V.T., McNicol, J.V., Razzo, R., et al., Assessing the Potential for Unintended Effects of Genetically Modified Potatoes Perturbed in Metabolic and Developmental Processes: Targeted Analysis of Key Nutrients and Anti-Nutrients, Transgenic Res., 2006, vol. 15, pp. 409–425.CrossRefGoogle Scholar
  6. 6.
    Kuznetsov, Vl.V., Kulikov, A.M., Mitrokhin, I.A., and Tsydendambaev, V.D., Genetically Modified Organisms and Biohazard, EKOS Inform., 2004, no. 10, pp.3–64.Google Scholar
  7. 7.
    Chyi Y.-S., Jorgensen, R.A., Goldstein, D. et al., Location and Stability of Agrobacterium-Mediated T-DNA Insertions in the Lycopersicon Genome, Mol. Gen. Genet., 1986, vol. 204, pp. 64–69.CrossRefGoogle Scholar
  8. 8.
    Raldugina, G.N., Gorelova, S.V., and Kozhemyakin, A.V., Stability and Inheritance of Transgenes in Rapeseed Plants, Fiziol. Rastenii, 2000, vol. 47, pp. 437–445.Google Scholar
  9. 9.
    Gao, J., Lee, J.M., and An, G., The Stability of Foreign Protein Production in Genetically Modified Plant Cells, Plant Cell Rep., 1991, vol. 10, pp. 533–536.CrossRefGoogle Scholar
  10. 10.
    Marenkova, T.V. and Deineko, E.V., A Change in the Stability of Marker nptII and uidA Gene Expression in Transgenic Tobacco Plants, Russ. J. Genet., 2006, vol. 42, no. 5, pp. 518–525.CrossRefGoogle Scholar
  11. 11.
    Duformantel, N., Tissot, G., Garcon, F., et al., Stability of Soybean Recombinant Plastome over Six Generations, Transgenic Res., 2006, vol. 15, pp. 305–311.CrossRefGoogle Scholar
  12. 12.
    Weekes, R., Deppe, C., Allnutt, T., et al., Crop-to-Crop Gene Flow Using Farm Scale Sites of Oilseed Rape (Brassica napus) in the UK, Transgenic Res., 2005, vol. 14, pp. 749–759.CrossRefPubMedGoogle Scholar
  13. 13.
    Zhang, W., Linscombe, S.D., Webster, E., et al., Risk Assessment of the Transfer of Imazethapyre Herbicide Tolerance from Clearfield Rice to Red Rice (Oryza sativa), Euphytica, 2006, vol. 152, pp. 75–86.CrossRefGoogle Scholar
  14. 14.
    Letanneur, J.C., Laredo, C., Jenczewski, E., and Monod, H., Modeling Gene Flow between Oilseed Rape and Wild Radish: I. Evolution of Chromosome Structure, Theor. Appl. Genet., 2007, vol. 114, pp. 209–221.PubMedGoogle Scholar
  15. 15.
    Yuan, Q.H., Shi, L., Wang, F., et al., Investigation of Rice Transgene Flow Compass Sectors by Using Male Sterile Line as a Pollen Detector, Theor. Appl. Genet., 2007, vol. 115, pp. 549–560.CrossRefPubMedGoogle Scholar
  16. 16.
    Angell, S.M. and Baulcombe, D.C., Consistent Gene Silencing in Transgenic Plants Expressing a Replicating Potato Virus X RNA, EMBO J., 1997, vol. 16, pp. 3675–3684.CrossRefPubMedGoogle Scholar
  17. 17.
    Iglesias, V.A., Moscone, E.A., Papp, I., et al., Molecular and Cytogenetic Analysis of Stably and Unstably Expressed Transgene Loci in Tobacco, Plant Cell, 1997, vol. 9, pp. 1251–1264.CrossRefPubMedGoogle Scholar
  18. 18.
    Meng, L., Ziv, M., and Lemaux, P.G., Nature of Stress and Transgene Locus Influences Transgene Expression Stability in Barley, Plant Mol. Biol., 2006, vol. 62, pp. 15–28.CrossRefPubMedGoogle Scholar
  19. 19.
    Belozersky, M.A.,. Dunaevsky, Y.E., Musolyamov, A.X., Egorov, T.A., Complete Amino Acid Sequence of the Protease Inhibitor from Buckwheat Seeds, FEBS Lett., 1995, vol. 371, pp. 264–266.CrossRefPubMedGoogle Scholar
  20. 20.
    Gamborg, O.L., Miller, R.A., and Ojima, K., Nutrient Requirements of Suspension Cultures of Soybean Root Cells, Exp. Cell Res., 1968, vol. 50, no. 2, pp. 150–155.Google Scholar
  21. 21.
    Horsch, R.B., Fry, J.E., Hoffman, N.L., et al., A Simple and General Method for Transferring Genes into Plants, Science, 1985, vol. 227, pp. 1229–1231.CrossRefGoogle Scholar
  22. 22.
    Olhoft, P.M. and Somers, D.A., L-Cystein Increases Agrobacterium-Mediated T-DNA Delivery into Soybean Cotyledonary-Node Cells, Plant Cell Rep., 2001, vol. 20, pp. 706–711.CrossRefGoogle Scholar
  23. 23.
    Khadeeva, N.V. and Kuzmina, N.A., Improvement of the Tobacco Bacterial Transformation Efficiency, in Tezisy dokladov V s“ezda fiziologov rastenii Rossii (Proceedings V Conference of Plant Physiologist of Russia), Pensa, 2003, pp. 496–497.Google Scholar
  24. 24.
    Cherednichenko, M.Yu., Transformation of Potato and Tobacco by Means of Defensine Genes and Protease Inhibitor BWI-1a, Cand. Sci. (Biol.) Dissertation, Moscow: Moscow Timiryazev Agricultural Academy, 2005.Google Scholar
  25. 25.
    Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 1982.Google Scholar
  26. 26.
    Pausheva, Z.P., Praktikum po tsitologii rastenii (Manual on Plant Cytology), Moscow: Kolos, 1974.Google Scholar
  27. 27.
    Rokitskii, P.F., Osnovy variatsionnoi statistiki dlya biologov (Principles of Variation Statistics for Biologists), Minsk: Beloruskiy Gos. Univ., 1961.Google Scholar
  28. 28.
    Maisuryan, A.N., Khadeeva, N.V., and Pogosov, V.N., Isolation of 5-Bromodeoxyuridine Resistant Cell Lines of Halploid Tobacco, Fiziol. Rastenii, 1981, vol. 28, no. 3, p. 555–561.Google Scholar
  29. 29.
    Bavrina, T.V., Milyaeva, E.L., and Romanov, G.A., Genetic Modification of Tobacco (Nicotiana tabacum L.), Resulting in Prolonged Blossoming, in Fiziologiya transgennogo rasteniya i problemy biobezopasnosti (Transgenic Plant Physiology and Biohazard Problem), Proc. 2 All-Union Symposium, Moscow, 2007, p. 16.Google Scholar
  30. 30.
    Deineko, E.V., Study of Heterological and Own Gene Expression among Transgenic Plant (by Example of Nicotiana tabacum L.), Doctoral (Biol.) Dissertation, Moscow: Vavilov Institute of General Genetics, 2004.Google Scholar
  31. 31.
    Ambros, P.F., Matzke, A.J.M., and Matzke, M.A., Localization of Agrobacterium rhizogenes T-DNA in Plant Chromosomes by in situ Hybridization, EMBO J., 1986, vol. 5, pp. 2073–2077.PubMedGoogle Scholar
  32. 32.
    Zambryski, P., Basic Processes Underlying Agrobacterium-Mediated DNA Transfer to Plant Cells, Annu. Rev. Genet., 1988, vol. 21, pp. 1–30.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Vavilov Institute of General GeneticsRussian Academy of SciencesMoscowRussia

Personalised recommendations