Russian Journal of Genetics

, Volume 46, Issue 1, pp 50–56 | Cite as

Inheritance of marker and target genes in seed and vegetative progenies of transgenic tobacco plants carrying the buckwheat serine protease inhibitor gene

Plant Genetics


The buckwheat serine protease inhibitor (BWI-1) target gene (ISP) was expressed under the control of the constitutive 35S promoter of the cauliflower mosaic virus was expressed in transgenic tobacco plants and conferred antibacterial resistance. A stable and linked inheritance and expression of the marker nptII and target genes were observed in a random sample of independent transgenic tobacco plants after longterm propagation by nodal segments or multiple (for 1.5 years) regenerations under nonselective conditions; the transgene insert was preserved in the T1 seed progeny. Transgenic plants displayed numerous alterations in microsporogenesis. A loss of kanamycin (Km) resistance was accompanied by a loss of antibacterial activity in two lines. Segregation was observed for Km resistance in line C7 and for seedling size in line C22.


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© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Vavilov Institute of General GeneticsRussian Academy of SciencesMoscowRussia

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