Russian Journal of Genetics

, Volume 46, Issue 1, pp 36–42 | Cite as

Using IRAP markers for analysis of genetic variability in populations of resource and rare species of plants

  • S. V. Boronnikova
  • R. N. Kalendar
Plant Genetics


Species-specific LTR retrotransposons were first cloned in five rare relic species of drug plants located in the Perm’ region. Sequences of LTR retrotransposons were used for PCR analysis based on amplification of repeated sequences from LTR or other sites of retrotransposons (IRAP). Genetic diversity was studied in six populations of rare relic species of plants Adonis vernalis L. by means of the IRAP method; 125 polymorphic IRAP-markers were analyzed. Parameters for DNA polymorphism and genetic diversity of A. vernalis populations were determined.


Retrotransposon Sequence Rare Plant Species IRAP Marker Inter Retrotransposon Amplify Polymor Phism Interpopulation Genetic Diversity 
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© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Biological Faculty, Department of Botany and Genetics of PlantsThe Perm’ State UniversityPerm’Russia
  2. 2.Institute of BiotechnologyUniversity of HelsinkiHelsinkiFinland

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