Advertisement

Russian Journal of Genetics

, 45:1368 | Cite as

Chromosome synteny of the a genome of two evolutionary wheat lines

  • O. B. Dobrovolskaya
  • P. Sourdille
  • M. Bernard
  • E. A. Salina
Experimental Articles

Abstract

In order to estimate synteny between At and A polyploid wheat genomes belonging to different evolutionary lines (Timopheevi and Emmer), saturation of chromosome maps of Triticum timopheevii At genome by molecular markers has been conducted. Totally, 179 EST-SSR and 48 genomic SSR-markers have been used with the following integration of 13 and 7 markers correspondingly into chromosome maps of At genome. ESTSSR showed higher transferability and lower polymorphism than genomic SSR markers. The chromosome maps designed were compared to maps of homoeologous chromosome group of the T. aestivum A genome. No disturbances of colinearity, i.e., of the order of markers within the chromosome segments on which they had been previously mapped, were observed. According to the quantity assessment of markers amplifying in homoeologous chromosomes, the maximum divergence was detected in two groups (4At/4A and 3At/3A) among the seven chromosomes examined in the A t and A genomes. Comparison of molecular genetic mapping results with the published results of studying meiosis of F1 hybrids and the frequency of chromosomes substitution in introgressive T. aestivum × T. timopheevii lines suggest that individual chromosomes of the At and A genomes evolve differently. Translocations were shown to introduce the major impact on the divergence of 4At/4A and 6At/6A chromosomes, while mutations of the primary DNA structure, on the divergence of homoeologous group 3 chromosomes. The level of reorganization of other chromosomes during the evolution in the At and A genomes was significantly lower.

Keywords

Common Wheat Wheat Genome Homoeologous Chromosome Polyploid Wheat Emmer Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bonierbale, M.W., Plaisted, R.L., and Tanksley, S.D., RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato, Genetics, 1988, vol. 120, pp. 1095–1103.PubMedGoogle Scholar
  2. 2.
    Chao, S., Sharp, P.J., Worland, A.J., and Warham, E.J., RFLP-Based Genetic Maps of Wheat Homoeologous Group 7 Chromosomes, Theor. Appl. Genet., 1989, vol. 78, pp. 495–504.CrossRefGoogle Scholar
  3. 3.
    Moore, G., Roberts, M., Aragon-Alcaide, L., and Foote, T., Centromeric Sites and Cereal Chromosome Evolution, Chromosoma, 1997, vol. 35, pp. 17–23.Google Scholar
  4. 4.
    Van Deynze, A.E., Nelson, J.C., Yglesias, E.S., et al., Comparative Mapping in Grasses: Wheat Relationships, Mol. Gen. Genet., 1995, vol. 248, pp. 744–754.CrossRefPubMedGoogle Scholar
  5. 5.
    Kurata, N., Moore, G., Nakamura, Y., et al., Conservation of Genomic Structure between Rice and Wheat, Biotechnol., 1994, vol. 12, pp. 276–278.CrossRefGoogle Scholar
  6. 6.
    Gale, M.D. and Devos, K.M., Comparative Genetics in the Grasses, Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 1971–1974.CrossRefPubMedGoogle Scholar
  7. 7.
    Endo, T.R. and Gill, B.S., The Detection Stocks of Common Wheat, J. Hered., 1996, vol. 87, pp. 295–307.Google Scholar
  8. 8.
    Qi, L.L., Echalier, B., Friebe, B., and Gill, B.S., Molecular Characterization of a Set of Wheat Deletion Stocks for Using in Chromosome Bin Mapping of ESTs, Funct. Integr. Genomics, 2003, vol. 3, pp. 39–55.PubMedGoogle Scholar
  9. 9.
    Qi, L.L., Echalier, B., Chao, S., et al., A Chromosome Bin Map of 16000 Expressed Sequence Tag Loci and Distribution of Genes among the Three Genomes of Polyploid Wheat, Genetics, 2004, vol. 168, pp. 701–712.CrossRefPubMedGoogle Scholar
  10. 10.
    Sourdille, P., Singh, S., Cadalen, T., et al., Microsatellite-Based Deletion Bin System for the Establishment of Genetic-Physical Map Relationships in Wheat (Triticum aestivum L.), Funct. Integr. Genomics, 2004, vol. 4, pp. 12–25.CrossRefPubMedGoogle Scholar
  11. 11.
    Akhunov, E.D., Akhunova, A.R., Linkiewicz, A.M., et al., Synteny Perturbations between Wheat Homoeologous Chromosomes Caused by Locus Duplications and Deletions Correlate with Recombination Rates, Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 10836–10841.CrossRefPubMedGoogle Scholar
  12. 12.
    Tsunewaki, K., Plasmon Analysis as the Counterpart of Genome Analysis, in Methods in Genome Analysis in Plants, Boca Raton: CRC, 1996, pp. 272–299.Google Scholar
  13. 13.
    Huang, S., Sirikhachornkit, A., Su, X.J., et al., Genes Encoding Plastid Acetyl-CoA Carboxylase and 3-Phosphoglycerate Kinase of the Triticum/Aegilops Complex and the Evolutionary History of Polyploid Wheat, Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 8133–8138.CrossRefPubMedGoogle Scholar
  14. 14.
    Levy, A.V. and Feldman, M., The Impact of Polyploidy on Grass Genome Evolution, Plant Physiol., 2002, vol. 130, pp. 1587–1593.CrossRefPubMedGoogle Scholar
  15. 15.
    Salina, E.A., Leonova, I.N., Efremova, T.T., and Röder, M.S., Wheat Genome Structure: Translocations during the Course of Polyploidization, Funct. Integr. Genomics, 2006, vol. 6, pp. 71–80.CrossRefPubMedGoogle Scholar
  16. 16.
    Nicot, N., Chiquet, V., Gandon, B., et al., Study of Simple Sequence Repeat (SSR) Markers from Wheat Expressed Sequence Tags (ESTs), Theor. Appl. Genet., 2004, vol. 109 P, pp. 800–805.CrossRefGoogle Scholar
  17. 17.
    Zhang, L.Y., Bernard, M., Leroy, P., et al., High Transferability of Bread Wheat EST-Derived SSRs to Other Cereals, Theor. Appl. Genet., 2005, vol. 111, pp. 677–687.CrossRefPubMedGoogle Scholar
  18. 18.
    Yu, J.-K., Dake, T., Singh, S., et al., Development and Mapping of EST-Derived Simple Sequence Repeat Markers for Hexaploid Wheat, Genome, 2004, vol. 47, pp. 805–818.CrossRefPubMedGoogle Scholar
  19. 19.
    Lander, E.S., Green, P., Abrahamson, J., et al., MAPMAKER: An Interactive Computer Package for Constructing Primary Genetic Linkage Maps of Experimental and Natural Populations, Genomics, 1987, vol. 1, pp. 174–181.CrossRefPubMedGoogle Scholar
  20. 20.
    Kosambi, D.D., The Estimation of Map Distances from Recombination Values, Ann. Eugen., 1943, vol. 12, pp. 172–175.Google Scholar
  21. 21.
    Dobrovolskaya, O., Boeuf, C., Salse, J., et al., Construction of the Ae. speltoides Microsatellite Maps and Map-Based Comparisons of the Related S, B, and G Genomes, Proceedings of 2nd Workshop TritGen COST Action FA2008, Christov, N.K. and Todorovska, E.G., Eds., Albena, 2008, p. 63.Google Scholar
  22. 22.
    Thiel, T., Michalek, W., Varshney, R.K., and Graner, A., Exploiting EST Databases for the Development and Characterization of Gene-Derived SSR-Markers in Barley (Hordeum vulgare), Theor. Appl. Genet., 2003, vol. 106, pp. 411–422.PubMedGoogle Scholar
  23. 23.
    Varshney, R.K., Sigmund, R., Börner, A., et al., Interspecific Transferability and Comparative Mapping of Barley EST-SSR Markers in Wheat, Rye and Rice, Plant Sci., 2005, vol. 168, pp. 195–202.CrossRefGoogle Scholar
  24. 24.
    Eujayl, I., Sorrells, M.E., Baum, P.M., et al., Isolation of EST-Derived Microsatellite Markers for Genotyping the A and B Genomes of Wheat, Theor. Appl. Genet., 2002, vol. 104, pp. 399–407.CrossRefPubMedGoogle Scholar
  25. 25.
    Gupta, P.K., Rustgi, S., Sharma, S., et al., Transferable EST-SSR Markers for the Study of Polymorphism and Genetic Diversity in Bread Wheat, Mol. Genet. Genomics, 2003, vol. 270, pp. 315–323.CrossRefPubMedGoogle Scholar
  26. 26.
    Peng, J.H. and Lapitan, N.L.V., Characterization of ESTDerived Microsatellites in the Wheat Genome and Development of eSSR Markers, Funct. Integr. Genomics, 2005, vol. 5, pp. 80–96.CrossRefPubMedGoogle Scholar
  27. 27.
    Varshney, R.K., Thiel, T., Stein, N., et al., In silico Analysis on Frequency and Distribution of Microsatellites in ESTs of Some Cereal Species, Cell. Mol. Biol. Lett., 2002, vol. 7, pp. 537–546.PubMedGoogle Scholar
  28. 28.
    Devos, K.M., Dubkovsky, J., Dvorak, J., et al., Structural Evolution of Wheat Chromosomes 4A, 5A, and 7B and Its Impact on Recombination, Theor. Appl. Genet., 1995, vol. 91, pp. 282–288.CrossRefGoogle Scholar
  29. 29.
    Miftahudin Ross, K., Ma, X.F., Mahmoud, A.A., et al., Analysis of Expressed Sequence Tag Loci on Wheat Chromosome Group 4, Genetics, 2004, vol. 168, pp. 651–663.CrossRefGoogle Scholar
  30. 30.
    Jiang, J. and Gill, B.S., Different Species-Specific Chromosome Translocation in Triticum timopheevii and T. turgidum Support Diphyletic Origin of Polyploid Wheats, Chromosome Res., 1994, vol. 2, pp. 59–64.CrossRefPubMedGoogle Scholar
  31. 31.
    Rodriguez, S., Perera, E., Maestra, B., et al., Chromosome Structure of Triticum timopheevii Relative to T. turgidum, Genome, 2000, vol. 43, pp. 923–930.CrossRefPubMedGoogle Scholar
  32. 32.
    Badaeva, E.D., Badaev, N.S., Gill, B.S., and Filatenko, A.A., Intraspecific Karyotype Divergence in Triticum araraticum, Plant Syst. Evol., 1994, vol. 192, no. 1, pp. 117–145.CrossRefGoogle Scholar
  33. 33.
    Gordeeva, E.I., Leonova, I.N., Kalinina, N.P., et al., Comparative Cytologic and Molecular Analysis of Introgressive Lines of Bread Wheat, Containing Genetic Material of T. timorheevii, Russ. J. Genet., 2009, vol. 45 (in print).Google Scholar
  34. 34.
    Badaeva, E.D., Prokofieva, Z.D., Bilinskaya, E.N., et al., Cytogenetic Analysis of Hybrids Resistant to Yellow Rust and Powdery Mildew Obtained by Crossing Common Wheat (Triticum aestivum L., AABBDD) with Wheats of the Timopheevi Group (AtAtGG), Russ. J. Genet., 2000, vol. 36, no. 12, pp. 1401–1410.CrossRefGoogle Scholar
  35. 35.
    Röder, M.S., Korzun, V., Wendehake, K., et al., A Microsatellite Map of Wheat, Genetics, 1998, no. 149, pp. 2007–2023.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • O. B. Dobrovolskaya
    • 1
  • P. Sourdille
    • 2
  • M. Bernard
    • 2
  • E. A. Salina
    • 1
  1. 1.Institute of Cytology and Genetics Siberian BranchRussian Academy of SciencesNovosibirskRussia
  2. 2.UMR INRA-UBP Genetics, Diversity and Ecophysiology of CerealsClermont-FerrandFrance

Personalised recommendations