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Russian Journal of Genetics

, 45:1256 | Cite as

Multifunctional regulatory mutation in Bacillus subtilis flavinogenesis system

  • R. A. Kreneva
  • D. V. Karelov
  • N. V. Korolkova
  • A. S. Mironov
  • D. A. Perumov
Short Communications

Abstract

Among Bacillus subtilis riboflavin-resistant mutants we identified one, which differed from other regulatory mutants by overproduction of riboflavin and simultaneous upregulation of the ribC gene encoding flavokinase/FAD-synthase. Genetic and biochemical analysis showed that the ribU1 mutation determines a trans-acting factor that simultaneously regulates activity of riboflavin and truB-ribC-rpsO operons. Regulatory activity of the ribU1 mutation comprises about 10% of Rfn element activity on interaction with flavins. The ribU1 mutation can be presumably ascribed to a gene of the transcriptional regulators family.

Keywords

Bacillus Subtilis Regulatory Mutation Leader Region Transcriptional Terminator Transcriptional Regulator Family 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • R. A. Kreneva
    • 1
  • D. V. Karelov
    • 1
  • N. V. Korolkova
    • 2
  • A. S. Mironov
    • 2
  • D. A. Perumov
    • 1
  1. 1.Department of Molecular and Radiation Biophysics, Konstantinov Institute of Nuclear PhysicsRussian Academy of SciencesGatchinaRussia
  2. 2.Research Institute of Genetics and Selection of Industrial MicroorganismsMoscowRussia

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