Advertisement

Russian Journal of Genetics

, Volume 45, Issue 5, pp 571–574 | Cite as

Genetic variation in Ural populations of the rare plant species Adenophora lilifolia (L.) DC. on the basis of analysis of polymorphism of ISSR markers

  • S. V. Boronnikova
Plant Genetics

Abstract

The genetic variation in four populations of Adenophora lilifolia (L.) DC., a rare plant species of the Perm region, was analyzed using 56 ISSR markers. The characteristics of DNA polymorphism and population genetic diversity were determined. These data demonstrate a high level of DNA polymorphism (P 95 = 82.14%). The studied A. lilifolia populations are weakly differentiated; the intrapopulation variation is the main contributor to the genetic variation.

Keywords

Inter Simple Sequence Repeat Total Genetic Diversity ISSR Marker ISSR Primer Unweighted Pair Group Method With Arithmetic Average 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Tikhonova, V.L., Strategiya mobilizatsii i sokhraneniya genofonda redkikh i ischezayushchikh vidov rastenii: Konservatsiya geneticheskikh resursov (Strategies of Mobilization and Conservation of the Gene Pools of Rare and Endangered Plant Species: Conservation of Genetic Resources), Pushchino, 1985.Google Scholar
  2. 2.
    Gorchakovskii, P.L. and Shurova, E.A., Redkie i ischezayushchie rasteniya Urala i Predural’ya (Rare and Endangered Species of Ural and Cis-Ural), Moscow: Nauka, 1982.Google Scholar
  3. 3.
    Osobo okhranyaemye prirodnye territorii Permskoi oblasti: Reestr (Special Protected Natural Territories of Perm Oblast), Perm: Knizhnyi mir, 2002.Google Scholar
  4. 4.
    Torres, A.M., Weeden, N.F., and Martin, A., Linkage among Isozyme, RFLP and RAPD Markers in Vicia faba, Theor. Appl. Genet., 1993, vol. 5, pp. 937–945.Google Scholar
  5. 5.
    Zietkiewicz, E., Rafalski, A., and Labuda, D., Genome Fingerprinting by Simple Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification, Genomics, 1994, vol. 20, pp. 176–183.PubMedCrossRefGoogle Scholar
  6. 6.
    Nei, M. and Li, W.-H., Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases, Proc. Natl. Acad. Sci. USA, 1979, vol. 76, pp. 5269–5273.PubMedCrossRefGoogle Scholar
  7. 7.
    Van de Peer, Y. and De Wachter, R., TREECON for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Comput. Applic. Biosci., 1994, vol. 10, no. 5, pp. 569–570.Google Scholar
  8. 8.
    Felsenstein, J., Confidence Limits in Phylogenies: An Approach Using Bootstrap, Evolution, 1985, vol. 39, no. 4, pp. 783–791.CrossRefGoogle Scholar
  9. 9.
    Zhivotovskii, L.A., Index of Population Similarity at Polymorphic Characters, Zh. Obshch. Biol., 1979, vol. 40, no. 4, pp. 587–602.Google Scholar
  10. 10.
    Zhivotovsky, L.A., Index of Within-Population Diversity, Zh. Obshch. Biol., 1980, vol. 41, no. 6, pp. 828–836.Google Scholar
  11. 11.
    Nei, M., Genetic Distance between Populations, Am. Natur., 1972, vol. 106, pp. 283–292.CrossRefGoogle Scholar
  12. 12.
    Nei, M., Molecular Evolutionary Genetics, New York: Columbia Univ. Press, 1987, pp. 176–187.Google Scholar
  13. 13.
    Krasnaya kniga Permskogo kraya (Red Book of Perm Krai), Shepel’, A.I., Ed., Perm: Knizhnyi mir, 2008.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.Department of Botany and Plant GeneticsPerm State UniversityPermRussia

Personalised recommendations