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Russian Journal of Genetics

, Volume 45, Issue 4, pp 440–444 | Cite as

Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences

  • Y. Diao
  • Y. Miao
  • X. Lin
  • C. Liao
  • F. Guo
  • Z. Hu
Plant Genetics
  • 119 Downloads

Abstract

In this study, the relationships of five varieties in Perilla frutescens (L.) Britton were analyzed. All the varieties showed the same chromosome number 2n = 40, and the banding patterns of PI-DAPI staining and the FISH results of 45S rDNA probe were on the same site of the chromosome pair. The 5S rRNA gene spacers were ranged from 413 bp in P. frutescens var. purpurascens and 408 bp in var. frutescens. The varieties analyzed here were clustered into two clades according to the phylogenetic analysis. The sequence identity of 96% between var. auriculato-dentata and var. arguta suggested they could be considered the same variety.

Keywords

Perilla Frutescens Fish Result Weedy Type Diploid Genome Donor Squalius Alburnoides 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • Y. Diao
    • 1
    • 2
  • Y. Miao
    • 1
  • X. Lin
    • 3
  • C. Liao
    • 3
  • F. Guo
    • 4
  • Z. Hu
    • 1
  1. 1.Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life ScienceWuhan UniversityWuhanChina
  2. 2.College of Life Science and TechnologyChongqing University of Arts and SciencesYongchuanChina
  3. 3.Institute of Chinese Herbal MedicinesHubei Academy of Agricultural SciencesEnshiChina
  4. 4.Perilla Research InstituteYunnan Agricultural UniversityKunmingChina

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