Russian Journal of Genetics

, Volume 45, Issue 3, pp 313–321 | Cite as

A study of the storage proteins in the introgression lines of common wheat (Triticum aestivum L. × T. timopheevii Zhuk.) Resistant to brown leaf rust

  • L. V. Obukhova
  • E. B. Budashkina
  • V. K. Shumny
Plant Genetics

Abstract

Storage proteins, prolamins, were studied in ten introgression lines of common wheat bred with involvement of Triticum timopheevii (Tt) Zhuk. and five commercial hexaploid wheat cultivars. The lines are resistant to leaf rust. A comparative analysis of the storage proteins in the Triticum aestivum L. (Ta) introgression lines and the parental forms allowed us to (1) detect the active genes of prolamins on the chromosomes homeologous groups 1 and 6 in the introgression lines of T. aestivum and T. timopheevii; (2) clarify their origin; (3) identify the chromosome attribution of the products; (4) estimate the degree of introgression and postulate the introgression mechanisms; and (5) predict the bread-making quality of these introgression lines.

Keywords

Storage Protein Leaf Rust Common Wheat Chinese Spring Introgression Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Dorofeev, V.F., Udachin, R.A., Semenova, L.V., et al., Pshenitsy mira (Wheats of the World), Dorofeev, V.F., Ed., Leningrad: Agropromizdat, 1987.Google Scholar
  2. 2.
    Budashkina, E.B. and Kalinina, N.P., Development of Introgressive Lines of Common Wheat (2n = 42) Resistant to Brown Rust, RF Patent 2138155, 1998.Google Scholar
  3. 3.
    Budashkina, E.B. and Kalinina, N.P., Development and Genetic Analysis of Common Wheat Introgressive Lines Resistant to Leaf Rust, Acta Phytopathologica Entomologica Hungarica, 2001, vol. 36, nos. 1–2, pp. 61–65.Google Scholar
  4. 4.
    Leonova, I.N., Roder, M.S., Budashkina, E.B., et al., Molecular Analysis of Leaf Rust-Resistant Introgression Lines Obtained by Crossing of Hexaploid Wheat Triticum aestivum with Tetraploid Wheat Triticum timopheevii, Russ. J. Genet., 2002, vol. 38, no. 12, pp. 1648–1655.CrossRefGoogle Scholar
  5. 5.
    Leonova, I.N., Borner, A., Budashkina, E., et al., Identification of Microsatellite Marker for a Leaf Rust Resistance Gene Introgressed into Common Wheat from Triticum timopheevii, Plant Breed., 2004, vol. 123, pp. 93–95.CrossRefGoogle Scholar
  6. 6.
    Shewry, P.R., Halford, N.G., and Tatham, A.S., The High Molecular Weight Subunits of Wheat, Barley and Rye: Genetics, Molecular Biology, Chemistry and Role in Wheat Gluten Structure and Functionality, Oxford Surveys Plant Mol. Cell. Biol., 1989, vol. 6, pp. 163–219.Google Scholar
  7. 7.
    Bushuk, W. and Zilman, R.R., Wheat Cultivar Indentification by Gliadin Electrophoregrams, Can. J. Plant Sci., 1978, vol. 58, pp. 505–515.CrossRefGoogle Scholar
  8. 8.
    Metakovsky, E.V., Novoselskaya, A.Yu., Kopus, M.M., et al., Blocks of Gliadin Components in Winter Wheat Detected by One-Dimensional Polyacrylamide Gel Electrophoresis, Theor. Appl. Genet., 1984, vol. 67, pp. 559–568.CrossRefGoogle Scholar
  9. 9.
    Sozinov, A.A., Polimorfizm belkov i ego znachenie v genetike i selektsii (Protein Polymorphism and Its Significance in Genetics and Breeding), Moscow: Nauka, 1985.Google Scholar
  10. 10.
    Konarev, V.G., Belki pshenitsy (Wheat Proteins), Moscow: Kolos, 1980.Google Scholar
  11. 11.
    Metakovsky, E.V., Gliadin Allele Identification in Common Wheat: II. Catalogue of Gliadin Alleles in Common Wheat, J. Genet. Breed., 1991, vol. 45, pp. 325–344.Google Scholar
  12. 12.
    Payne, P.I., Endosperm Proteins, Plant Gene Research: A Genetic Approach to Plant Biochemistry, Blonstein, A.D. and King, P.J., Eds., London: Springer-Verlag, 1986, pp. 207–231.Google Scholar
  13. 13.
    Shewry, P.R. and Tatham, A.S., Disulphide Bonds in Wheat Gluten Proteins, J. Cer. Sci., 1997, vol. 25, pp. 207–227.CrossRefGoogle Scholar
  14. 14.
    Singh, N.K. and Shepherd, K.W., Linkage Mapping of Genes Controlling Endosperm Storage Proteins in Wheat, Theor. Appl. Genet., 1988, vol. 75, pp. 628–641.CrossRefGoogle Scholar
  15. 15.
    Payne, P.I., Corfield, K.G., and Blackman, J.A., Correlation between the Inheritance of Certain High-Molecular-Weight Subunits of Glutenin and Bread-Making Quality in Progenies of Six Crosse of Bread Wheat, J. Sci. Food Agric., 1981, vol. 32, pp. 51–60.CrossRefGoogle Scholar
  16. 16.
    Wrigley, C.W., Lawrence, G.J., and Shepherd, K.W., Association of Glutenin Subunits with Gliadin Composition and Grain Quality in Wheat, Aust. J. Plant Physiol., 1982, vol. 9, pp. 15–30.CrossRefGoogle Scholar
  17. 17.
    Payne, P.I., Nightingale, M.A., Krattiger, A.F., and Holt, L.M., The Relationship between HMW Glutenin Subunit Composition and the Bread-Making Quality of British-Grown Wheat Varieties, J. Sci. Food Agric., 1987, vol. 40, pp. 51–65.CrossRefGoogle Scholar
  18. 18.
    Gupta, R.B., Singh, N.K., and Sheperd, K.W., The Cumulative Effects of Allelic Variation in LMW and HMW Glutenin Subunits on Physical Dough Properties in the Progeny of Two Bread Wheats, Theor. Appl. Genet., 1989, vol. 77, pp. 57–62.CrossRefGoogle Scholar
  19. 19.
    Gupta, R.B. and Mac Ritchie, F., Allelic Variation at Glutenin Subunit and Gliadin Loci, Glu-1, Glu-3, and Gli-1, of Common Wheat: II. Biochemical Basis of the Allelic Effects on Dough Properties, J. Cereal Sci., 1994, vol. 19, pp. 19–29.CrossRefGoogle Scholar
  20. 20.
    Luo, C., Griffin, W.B., Branlard, G., and McNeil, D.L., Comparison of Low- and High Molecular-Weight Wheat Glutenin Allele Effects on Flour Quality, Theor. Appl. Genet., 2001, vol. 102, pp. 1088–1098.CrossRefGoogle Scholar
  21. 21.
    Redaelli, R., Pogna, N.E., and Ng, P.K.W., Effects of Prolamins Encoded by Chromosomes 1B and 1D on the Rheological Properties of Dough in Near-Isogenic Lines of Bread Wheat, Cereal Chem., 1997, vol. 74, pp. 102–107.CrossRefGoogle Scholar
  22. 22.
    Obukhova, L.V., Budashkina, E.B., Ermakova, M.F., et al., Quality of Grain and Flour from Common Wheat Triticum timopheevii Introgressive Lines Resistant to Leaf Rust, S.-kh. Biol., 2008, no. 5, pp. 38–42.Google Scholar
  23. 23.
    Obukhova, L.V., Maystrenko, O.I., Generalova, G.V., et al., Role of Prolamins in BMQ of Wheat Substitution Lines Raised from Cultivars Contrasting in This Quality, Cereal Res. Commun., 2001, vol. 29, nos. 1–2, pp. 189–196.Google Scholar
  24. 24.
    Novoselskaya, A.Yu., Metakovsky, E.V., and Sozinov, A.A., Polymorphism Study of Some Wheat Varieties by Means of One-Dimensional and Two-Dimensional Electrophoresis, Tsitol. Genet., 1983, vol. 17, pp. 45–53.Google Scholar
  25. 25.
    Metakovsky, E.V. and Novoselskaya, A.Yu., Gliadin Allele Identification in Common Wheat: I. Methodological Aspects of the Analysis of Gliadin Patterns by One-Dimensional Polyacrylamide Gel Electrophoresis, J. Genet. Breed., 1991, vol. 45, no. 4, pp. 317–324.Google Scholar
  26. 26.
    Morgunov, A.I., Rogers, W.J., Sayers, E.J., and Metakovsky, E.V., The High-Molecular-Weight Glutenin Subunit Composition of Soviet Wheat Varieties, Euphytica, 1990, vol. 51, pp. 41–52.CrossRefGoogle Scholar
  27. 27.
    Sozinov, A.A., Metakovsky, E.V., and Koval, S.F., Genotype Formation Pattern upon Wheat Breeding, Vestn. S-kh. Nauki, 1986, no. 3(354), pp. 60–70.Google Scholar
  28. 28.
    Novoselskaya, A.Yu., Metakovsky, E.V., Koval, S.F., and Sozinov, A.A., Genetic Control of Gliadin Composition in Spring Common Wheat Variety Novosibirskaya 67, Dokl. Akad. Nauk SSSR, 1985, vol. 281, pp. 702–704.Google Scholar
  29. 29.
    Chernakov, V.M. and Metakovsky, E.V., Diversity of Gliadin-Coding Locus Allelic Variants and Evaluation of Genetic Similarity of Common Wheat Varieties from Different Breeding Counters, Russ. J. Genet., 1994, vol. 30, no. 4, pp. 509–517.Google Scholar
  30. 30.
    Payne, P.I. and Lawrence, G.J., Catalogue of Alleles for the Complex Gene Loci, Glu-A1, Glu-B1, and Glu-D1 Which Code for High-Molecular-Weight Subunits of Glutenin in Hexaploid Wheat, Cereal Res. Commun., 1983, vol. 11, pp. 29–35.Google Scholar
  31. 31.
    Gupta, R.B. and Shepherd, K.W., Two-Step One-Dimensional SDS-PAGE Analysis of LMW Subunits of Glutelin 1: Variation and Genetic Control of the Subunits in Hexaploid Wheats, Theor. Appl. Genet., 1990, vol. 80, pp. 65–74.Google Scholar
  32. 32.
    Dragovich, A.Yu., Zima, V.G., Fisenko, A.V., et al., A Comparison of Two Existing Catalogs of the Alleles of Gliadin-Coding Loci in Winter Common Wheat, Russ. J. Genet., 2006, vol. 42, no. 8, pp. 1107–1116.CrossRefGoogle Scholar
  33. 33.
    Galili, G. and Feldman, M., Genetic Control of Endosperm Proteins in Wheat: 1. The Use of High Resolution One-Dimensional Gel Electrophoresis for the Allocation of Genes Coding for Endosperm Protein Subunits in the Common Wheat Cultivar Chinese Spring, Theor. Appl. Genet., 1983, vol. 64, pp. 97–101.CrossRefGoogle Scholar
  34. 34.
    Yakobashvili, Z.A., Determination of Phylogenetic Relations between Wheat Species by the Analysis of Polymorphism and Inheritance of Storage Proteins, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Moscow: Inst. Gen. Genet., 1989, p. 20.Google Scholar
  35. 35.
    Gu, Y.Q., Coleman-Derr, D., Kong, X., and Anderson, O.D., Rapid Genome Evolution Revealed by Comparative Sequence Analysis of Orthologous Regions from Four Triticeae Genomes, Plant Physiol., 2004, vol. 135, pp. 459–470.PubMedCrossRefGoogle Scholar
  36. 36.
    Enno, T., Peusha, H., Timofeyeva, L., et al., Identification of Chromosomal Translocations in Common Wheat, Derivative of Triticum timopheevii, Acta Agronomica Hungarica, 1998, vol. 46, no. 3, pp. 209–216.Google Scholar
  37. 37.
    Järve, K., Peusha, H.O., Tsymbalova, J., et al., Chromosomal Location of a Triticum timopheevii-Derived Powdery Mildew Resistance Gene Transferred to Common Wheat, Genome, 2000, vol. 43, pp. 377–381.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • L. V. Obukhova
    • 1
  • E. B. Budashkina
    • 1
  • V. K. Shumny
    • 1
  1. 1.Institute of Cytology and GeneticsRussian Academy of SciencesNovosibirskRussia

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