Russian Journal of Genetics

, Volume 44, Issue 1, pp 35–43 | Cite as

Genetic polymorphism of Siberian spruce (Picea obovata Ledeb.) in Middle Siberia

  • A. N. Kravchenko
  • A. Ya. Larionova
  • L. I. Milyutin
Plant Genetics


Based on the analysis of 22 loci controlling allozyme variation of MDH, SKDH, 6-PGD, IDH, PEPCA, GOT, FDH, LAP, PGI, PGM, SOD, and GDH, the data on within-and among-population variation for nine cenopopulations of Siberian spruce (Picea obovata Ledeb.) located along the Yenisei mieridian, from 65°50′ NL to 52°14′NL, were obtained. It was demonstrated that 86.36% of the loci, tested in the species analyzed, were polymorphic. The mean number of alleles per locus was 2.91, and the observed and expected heterozygosity constituted 0.161 and 0.168, respectively. More than 97% of total variation occurred within the populations, while the proportion of the among-population variation constituted only 2.3% (F st =0.0230). Genetic distance (D N) between the populations examined varied from 0.0019 to 0.0115, averaging at 0.0051. It was shown that in the part of the Siberian spruce range examined there was no close association between the level of the genetic differentiation of the populations and the geographic distance between them. It seems likely, that this finding can be associated with the fact that Siberian spruce, growing on this territory, is the intrazonal species, i.e., it is not attached to a certain forest zone, and its distribution is mostly determined by local ecological conditions.


Genetic Distance Genetic Structure Introgressive Hybridization Isozyme Locus Krasnoyarsk Krai 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bobrov, E.G., Lesoobrazuyushchie khvoinye SSSR (Forest-Forming Conifers of the Soviet Union), Leningrad: Nauka, 1978.Google Scholar
  2. 2.
    Sokolov, V.A., Osnovy upravleniya lesami Sibiri (Basics of Forest Management in Siberia), Krasnoyarsk: Izd. SO RAN, 1997.Google Scholar
  3. 3.
    Rysin, L.P. and Savel’eva, L.I., Elovye lesa Rossii (Spruce Forests of Russia), Moscow: Nauka, 2002.Google Scholar
  4. 4.
    Polikarpov, N.P., Chebakova, N.M., and Nazimova, D.I., Klimat i gornye lesa Yuzhnoi Sibiri (Climate and Mountain Forests of South Siberia), Novosibirsk: Nauka, 1986.Google Scholar
  5. 5.
    Pravdin, L.F., El’ evropeiskaya i el’ sibirskaya v SSSR (Norway Spruce and Siberian Spruce in the Soviet Union), Moscow: Nauka, 1975.Google Scholar
  6. 6.
    Mamaev, S.A. and Popov, P.P., El’ sibirskaya na Urale (vnutrividovaya izmenchivost’ i struktura populyatsii) (Norway Spruce in the Urals (Intraspecies Variations and Population Structure), Moscow: Nauka, 1989.Google Scholar
  7. 7.
    Govorin, G.M., Siberian Spruce Variation in the Yenisei Basin, Lesovedenie, 1992, no. 5, pp. 56–59.Google Scholar
  8. 8.
    Putenikhin, V.P., Phenotypic Analysis of Siberian Spruce in the Southern Urals: Population Structure, Lesovedenie, 1997, no. 6, pp. 37–49.Google Scholar
  9. 9.
    Putenikhin, V.P., Shigapov, Z.Kh., and Farukshina, G.G., El’ sibirskaya na Yuzhnom Urale i v Bashkirskom Predural’e (populyatsionno-geneticheskaya struktura) (Siberian Spruce in the Southern Urals and the Bashkirian Cisurals (Population Genetic Structure)), Moscow: Nauka, 2005.Google Scholar
  10. 10.
    Popov, P.P., El’ evropeiskaya i sibirskaya (European and Siberian Spruces), Novosibirsk: Nauka, 2005.Google Scholar
  11. 11.
    Putenikhin, V.P., Yanbaev, Yu.A., Shigapov, Z.Kh., et al., Population Genetic Structure of Siberian Spruce in the Southern Urals: Diversity, Differentiation and Introgressiv Hybridization, in Biologicheskoe raznoobrazie: Introduktsiya rastenii (Biological Diversity: Plant Introduction), Proc. Sci. Conf., St. Petersburg, 1995, pp. 26–27.Google Scholar
  12. 12.
    Krutovskii, K.V. and Bergmann, F., Introgressive Hybridization and Phylogenetic Relationships between Norway, Picea abies (L.) Karst., and Siberian, P. obovata Ledeb. Spruce Species Studied by Isozyme Loci, Heredity, 1995, vol. 74, no. 5, pp. 464–480.CrossRefGoogle Scholar
  13. 13.
    Yanbaev, Yu.A., Shigapov, Z.Kh., Putenikhin, V.P., and Bakhtiyarova, R.M., Differentiation of Populations of Siberian Spruce, Picea obovata Ledeb., in the Southern Urals, Russ. J. Genet., 1997, vol. 33, no. 9, pp. 1060–1064.Google Scholar
  14. 14.
    Politov, D.V. and Krutovskii, K.V., Clinal Variation and Introgressive Hybridization among Populations of the Norway and Siberian Spruce, Zhizn’ populyatsii v geterogennoi srede (Populations in the Heterogenic Environment), Isohkar-Ola: Periodika Marii-El, 1998, vol. 2, pp. 78–89.Google Scholar
  15. 15.
    Yanbaev, Yu.A., Kosarev, M.N., and Bakhtiyarova, R.M., Genetic Structure of Siberian Spruce Populations near the Shul’gan-Tash Reserve, Izuchenie prirody v zapovednikakh Bashkortostana (Studying the Wild-Life in Bashkortostan Natural Reserves), Miass: Geotur, 1999, pp. 171–174.Google Scholar
  16. 16.
    Ganiev, Z.M., Spatial Structuredness of Genetic Variation among Forest-Forming Species in the Southern Urals, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Ufa: Bashkirian State Univ., 2000.Google Scholar
  17. 17.
    Kamalova, I.I., Iroshnikov, A.I., Vnukova, N.I., and Karachanskaya, S.I., The Effect of Felling on Spruce Forests’ Genetic Structure, in Lesnaya genetika i selektsiya na rubezhe tysyacheletii (Forest Genetics and Breeding at the Millenium Border), Proc. Conf., Voronezh: NIILGiS, 2001, p. 14.Google Scholar
  18. 18.
    Yanbaev, Yu.A., Ecological Population Aspects of Forest-Forming Species Adaptations to Natural and Technogenic Environment, Extended Abstract of Doctoral (Biol.) Dissertation, Tolyatti: Inst. of Ecology of the Volga River Basin RAS, 2002.Google Scholar
  19. 19.
    Shigapov, Z.Kh., Intraspecific Variations and Species Differentiation in the Family Pinaceae in the Urals, Extended Abstract of Doctoral (Biol.) Dissertation, Perm: Perm State Univ., 2005.Google Scholar
  20. 20.
    Lavrinenko, O.V. and Lavrinenko, I.A., Clonal Structure and Variation of Siberian Spruce (Picea obovata Ledeb.) in Isolated Populations at the Northernmost Limit of Its Geographical Range, Siberian Ekol. Zh., 2004, vol. 11, no. 2, pp. 179–190.Google Scholar
  21. 21.
    Goncharenko, G.G. and Potenko, V.V., Parameters of Genetic Variation and Differentiation in the Populations of Norway Spruce (Picea abies (L.) Karst.) and Siberian Spruce (Picea obovata Ledeb.), Genetika (Moscow), 1991, vol. 27, no. 10, pp. 1759–1772.Google Scholar
  22. 22.
    Larionova, A.Ya., Allozymic Variation in Siberian Spruce (Picea obovata Ledeb.) in the Middle Siberia, in Bioraznoobrazie i redkie vidy rastenii Srednei Sibiri (Biodiversity and Rare Plant Species of the Middle Siberia), Krasnoyarsk: IL SO RAN, 1995, pp. 50–52.Google Scholar
  23. 23.
    Goncharenko, G.G. and Padutov, V.E., Populyatsionnaya i evolyutsionnaya genetika elei Palearktiki (Population and Evolutional Genetics of Palearctic Spruces), Gomel’: IL NAN Belarus, 2001.Google Scholar
  24. 24.
    Padutov, V.E., Genetic Resources and Taxonomic Relationships of Main Forest-Forming Conifer Species of Eastern Europe, Siberia and Adjacent Regions (by the Example of Genera Picea and Pinus), Extended Abstract of Doctoral (Biol.) Dissertation, Minsk: Inst. of Genetics and Cytology NAS Belarus, 2002.Google Scholar
  25. 25.
    Potenko, V.V., Isozyme Polymorphism and Phylogenetic Interrelations of Conifer Species of the Russian Far East, Extended Abstract of Doctoral (Biol.) Dissertation, Vladivostok: Biology-Soil Science Inst. Far East Branch RAS, 2004.Google Scholar
  26. 26.
    Clayton, J.W. and Tretiak, D.N., Amino-Citrate Buffer for pH Control in Starch Gel Electrophoresis, J. Fish. Res. Board Canada, 1972, vol. 29, pp. 1169–1172.Google Scholar
  27. 27.
    Ridgway, G.J., Sherburne, S.W., and Lewis, R.D., Polymorphism in the Esterases of Atlantic Herring, Trans. Am. Fish. Soc., 1970, vol. 99, pp. 147–151.CrossRefGoogle Scholar
  28. 28.
    Markert, C.L. and Faulhaber, I., Lactate Dehydrogenase Isozyme Patterns in Fish, J. Exptl. Zool., 1965, vol. 159, no. 2, pp. 319–332.CrossRefGoogle Scholar
  29. 29.
    Brewer, G.J., Introduction to Isozyme Techniques, New York: Academic, 1970.Google Scholar
  30. 30.
    Vallejos, C.E., Enzyme Activity Staining, in Isozymes in Plant Genetics and Breeding, Amsterdam: Elsevier, 1983, pp. 469–516.Google Scholar
  31. 31.
    Goncharenko, G.G. and Padutov, V.E., Rukovodstvo po issledovaniyu drevesnykh vidov metodom elektroforeticheskogo analiza izofermentov (Guide to Arboreal Species Study by Means of Isozyme Electrophoretic Analysis), Gomel’: BelNIILKh, 1988.Google Scholar
  32. 32.
    Manchenko, G.P., Handbook of Detection of Enzymes on Electrophoretic Gels, CRC Press, 1994.Google Scholar
  33. 33.
    Ayala, F.J., Population and Evolutionary Genetics: A Primer, Menlo Park: Benjamin-Cummings, 1982.Google Scholar
  34. 34.
    Ayala, F.J. and Kiger, J., Modern Genetics, San Francisco: Benjamin-Cummings, 1984, 2nd ed., vol. 3.Google Scholar
  35. 35.
    Guries, R.P. and Ledig, F.T., Genetic Diversity and Population Structure in Pitch Pine (Pinus rigida Mill.), Evolution, 1982, vol. 36, pp. 387–402.CrossRefGoogle Scholar
  36. 36.
    Nei, M., Genetic Distance between Populations, Am. Nat., 1972, vol. 106, pp. 283–292.CrossRefGoogle Scholar
  37. 37.
    Yeh, F.C., Yang, R., and Boyle, T., POPGENE Version 1.32: Microsoft Windows Based Freeware for Population Genetic Analysis, 1999.Google Scholar
  38. 38.
    Sneath, P.H.A. and Sokal, R.R., Numerical Taxonomy: the Principles and Practice of Numerical Classification, San Francisco: Freeman, 1973.Google Scholar
  39. 39.
    Felsenstein, J., PHYLIP—Phylogeny Inference Package (Version 3.2), Cladistics, 1989, vol. 5, pp. 164–166.Google Scholar
  40. 40.
    Sukachev, V.N., Lesnye porody: ikh sistematika i fitosotsiologiya (Forest Tree Species: Their Systematics and Phytosociology), Moscow: Novaya Derevnya, 1928.Google Scholar
  41. 41.
    Milyutin, L.I. and Terent’ev, V.I., Geographical Spruce Cultures in Krasnoyarsk Krai, Lesovedenie, 1999, no. 4, pp. 16–22.Google Scholar
  42. 42.
    Laukhin, S.A. and Popov, P.P., Modern Late Pleistocene Spruce in Eastern Siberia, Lesovedenie, 2006, no. 2, pp. 61–64.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2008

Authors and Affiliations

  • A. N. Kravchenko
    • 1
  • A. Ya. Larionova
    • 1
  • L. I. Milyutin
    • 1
  1. 1.Sukachev Institute of ForestSiberian Branch of Russian Academy of SciencesKrasnoyarskRussia

Personalised recommendations