Russian Journal of Genetics

, Volume 43, Issue 11, pp 1306–1313 | Cite as

Phylogeny of the genus Lophozia (Dumort.) Dumort. s. str. inferred from nuclear and chloroplast sequences ITS1-2 and TRNL-F

  • A. A. Vilnet
  • I. A. Milyutina
  • N. A. Konstantinova
  • M. S. Ignatov
  • A. V. Troitsky
Plant Genetics


Maximum parsimony and maximum likelihood phylogenetic trees were constructed for 21 taxa of Lophozia s. str. and the related genera, Schistochilopsis (5 species), Protolophozia elongata, and Obtusifolium obtusum based on combined nuclear ITS1-2 and chloroplast trnL-F DNA sequences. The trees were characterized by similar topology. It was demonstrated that the genus Lophozia s. str. was monophyletic, excluding L. sudetica, which deserved isolation into a distinct cryptic genus. The species distribution among the clades disagreed with the sections distinguished based on anatomical and morphological data. The relationships within the genus Schistochilopsis were consistent with the sectioning of the genus, based on morphological characters. Analysis of molecular data provided more precise definition of the systematic position of a number of taxa. A low level of genetic divergence of geographically distant forms was demonstrated.


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  1. 1.
    Schuster, R.M., The Hepaticae and Anthocerotae of North America East of the Hundredth Meridian, New York, 1969, vol. 2.Google Scholar
  2. 2.
    Schuster, R.M. and Damsholt, K., The Hepaticae of West Greenland from ca. 66°N to 72°N, Meddel. om Groenland, 1974, vol. 199, no. 1, pp. 1–373.Google Scholar
  3. 3.
    Schljakov, R.N., Pechenochnye mkhi severa SSSR (The Liverworts of the North of the Soviet Union), issue 3: Pechenochniki: lofozievye, mezoptikhievye (Hepaticae: Lophoziaceae, Mezoptihiaceae), Leningrad, 1980.Google Scholar
  4. 4.
    Bakalin, V.A., Monograficheskaya obrabotka roda Lophozia (Dum.) Dum. s. str. (Lophoziaceae, Hepaticae) (Monographic Treatment of the Genus Lophozia (Dum.) Dum. s. str. (Lophoziaceae, Hepaticae)), Moscow: Nauka, 2005.Google Scholar
  5. 5.
    Grolle, R., Hepatics of Europe Including the Azores: An Annotatated List of Species, with Synonyms from the Recent Literature, J. Bryol., 1983, vol. 12, pp. 403–459.Google Scholar
  6. 6.
    Grolle, R. and Long, D.G., An Annotated Check-List of the Hepaticae and Anthocerotae of Europe and Macaronesia, J. Bryol., 2000, vol. 22, pp. 103–141.Google Scholar
  7. 7.
    Paton, J.A., The Liverwort Flora of the British Isles, Colshester: Harley, 1999.Google Scholar
  8. 8.
    Damsholt, K., Illustrated Flora of Nordic Liverworts and Hornworts, Lund: Nord. Bryol. Soc., 2002.Google Scholar
  9. 9.
    Buch, H., Vorarbeiten zu einer Lebermoosflora Fenno-Skandias: I. Ein Verzuch zur Aufteilung Gattungen Lophozia Dum. und Sphenolobus Steph., Mem. Soc. Fauna Flora Fenn., 1933, vol. 8, pp. 282–297.Google Scholar
  10. 10.
    Arnell, S., Hepaticae, in Illustrated Moss Flora of Fenoscandia, Lund 1956, pp. 1–308.Google Scholar
  11. 11.
    Potemkin, A. D. About the Systematic Position and Taxonomic Status of Genera Schistochilopsis (N. Kitag.) Konstantinova and Obtusifolium S.W. Arnell (Lorhoziaseae, Hepaticae), Novosti Sistematiki Nizshikh Rasteniy, 2005, vol. 38, pp. 362–365.Google Scholar
  12. 12.
    Yatsentyuk, N.A. Konstantinova, M.S. Ignatov, M.S., et al., On Phylogeny of Lophoziaceae and Related Families (Hepaticae, Jungermanniales) Based on trnL-trnF Intron-Spacer Sequences Of Chloroplast DNA, Molecular Systematics of Bryophytes: Progress, Problems and Perspectives, Goffinet, B., Hollowell, V., and Magill, R., Eds., Monographs in Systematic Botany from the Missouri Botanical Garden, vol. 98, St. Louis: Missouri Botanical Garden Press, 2004, pp. 150–167.Google Scholar
  13. 13.
    Doyle, J.J. and Doyle, J.L., A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue, Phytochem. Bull., 1987, vol. 19, pp. 11–15.Google Scholar
  14. 14.
    White, T.J., Bruns, T., Lee, S., and Taylor, J., Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics, PCR Protocols: A Guide to Methods and Applications, Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J., Eds., San Diego: Academic, 1990, pp. 315–322.Google Scholar
  15. 15.
    Taberlet, P., Gielly, L., Pautou, G., and Bouvet, J., Universal Primers for Amplification of Three Noncoding Regions of Chloroplast DNA, Plant Mol. Biol., 1991, vol. 17, pp. 1105–1109.PubMedCrossRefGoogle Scholar
  16. 16.
    Hall, T.A., BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT, Nucleic Acids Symp., 1999, ser. 41, pp. 95–98.Google Scholar
  17. 17.
    Goloboff, P.A., Farris, J.S., and Nixon, K.C., TNT: Tree Analysis Using New Technology (BETA) ver. Xxx. 2000, Tucuman: Published by the Authors, 1999.Google Scholar
  18. 18.
    Guindon, S. and Gascuel, O., A Simple, Fast and Accurate Algorithm to Estiamte Large Phylogenies by Maximum Likelihood, Systematic Biol., 2003, vol. 52, pp. 696–704.CrossRefGoogle Scholar
  19. 19.
    Keane, T.M., Naughton, T.J., and McInerney, J.O., Model-Generator: Amino Acid and Nucleotide Substitution Model Selection, Maynooth: National University Ireland, 2004.Google Scholar
  20. 20.
    Konstantinova, N.A., Potemkin, A.D., and Schljakov, R.N., Check-List of the Hepaticae and Anthocerotae of the Former USSR, Arctoa, 1992, vol. 1, nos. 1–2, pp. 87–127.Google Scholar
  21. 21.
    Schuster, R.M., Notes on Nearctic Hepaticae: III. A Conspectus of the Family Lophoziaceae, with a Revision of the Genera and Subgenera, Amer. Midland Naturalist, 1951, vol. 45, no. 1, pp. 1–89.CrossRefGoogle Scholar
  22. 22.
    Bisang, I., Biosystematische Studien an Lophozia subgen. Schistochilopsis (Hepaticae), Bryophytorum Bibilotheca, 1991, vol. 43, pp. 1–187.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2007

Authors and Affiliations

  • A. A. Vilnet
    • 1
  • I. A. Milyutina
    • 2
  • N. A. Konstantinova
    • 1
  • M. S. Ignatov
    • 3
  • A. V. Troitsky
    • 2
  1. 1.Polar-Alpine Botanical Garden-Institute of Kola Research CenterRussian Academy of SciencesKirovskRussia
  2. 2.Belozersky Institute of Physicochemical BiologyMoscow State UniversityMoscowRussia
  3. 3.Main Botanical Garden of Russian Academy of SciencesMoscowRussia

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